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Biophysical characterization of Atg11, a scaffold protein essential for selective autophagy in yeast
Autophagy is an intracellular degradation system in which the formation of an autophagosome is a key event. In budding yeast, autophagosomes are generated from the preautophagosomal structure (PAS), in which Atg11 and Atg17 function as scaffolds essential for selective and nonselective types of auto...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5757174/ https://www.ncbi.nlm.nih.gov/pubmed/29321961 http://dx.doi.org/10.1002/2211-5463.12355 |
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author | Suzuki, Hironori Noda, Nobuo N. |
author_facet | Suzuki, Hironori Noda, Nobuo N. |
author_sort | Suzuki, Hironori |
collection | PubMed |
description | Autophagy is an intracellular degradation system in which the formation of an autophagosome is a key event. In budding yeast, autophagosomes are generated from the preautophagosomal structure (PAS), in which Atg11 and Atg17 function as scaffolds essential for selective and nonselective types of autophagy, respectively. Structural studies have been extensively performed on Atg17, but not on Atg11, preventing us from understanding the selective type of the PAS. Here, we purified and characterized Atg11. Biophysical analyses, including analytical ultracentrifugation and CD, showed that Atg11 behaves as an elongated homodimer abundant in α‐helices in solution. Moreover, truncation analyses suggested that Atg11 has a parallel coiled‐coil architecture, in contrast to the antiparallel dimeric architecture of Atg17. |
format | Online Article Text |
id | pubmed-5757174 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-57571742018-01-10 Biophysical characterization of Atg11, a scaffold protein essential for selective autophagy in yeast Suzuki, Hironori Noda, Nobuo N. FEBS Open Bio Research Articles Autophagy is an intracellular degradation system in which the formation of an autophagosome is a key event. In budding yeast, autophagosomes are generated from the preautophagosomal structure (PAS), in which Atg11 and Atg17 function as scaffolds essential for selective and nonselective types of autophagy, respectively. Structural studies have been extensively performed on Atg17, but not on Atg11, preventing us from understanding the selective type of the PAS. Here, we purified and characterized Atg11. Biophysical analyses, including analytical ultracentrifugation and CD, showed that Atg11 behaves as an elongated homodimer abundant in α‐helices in solution. Moreover, truncation analyses suggested that Atg11 has a parallel coiled‐coil architecture, in contrast to the antiparallel dimeric architecture of Atg17. John Wiley and Sons Inc. 2017-12-04 /pmc/articles/PMC5757174/ /pubmed/29321961 http://dx.doi.org/10.1002/2211-5463.12355 Text en © 2017 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Suzuki, Hironori Noda, Nobuo N. Biophysical characterization of Atg11, a scaffold protein essential for selective autophagy in yeast |
title | Biophysical characterization of Atg11, a scaffold protein essential for selective autophagy in yeast |
title_full | Biophysical characterization of Atg11, a scaffold protein essential for selective autophagy in yeast |
title_fullStr | Biophysical characterization of Atg11, a scaffold protein essential for selective autophagy in yeast |
title_full_unstemmed | Biophysical characterization of Atg11, a scaffold protein essential for selective autophagy in yeast |
title_short | Biophysical characterization of Atg11, a scaffold protein essential for selective autophagy in yeast |
title_sort | biophysical characterization of atg11, a scaffold protein essential for selective autophagy in yeast |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5757174/ https://www.ncbi.nlm.nih.gov/pubmed/29321961 http://dx.doi.org/10.1002/2211-5463.12355 |
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