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On revealing the gene targets of Ebola virus microRNAs involved in the human skin microbiome

Ebola virus, a negative-sense single-stranded RNA virus, causes severe viral hemorrhagic fever and has a high mortality rate. Histopathological and immunopathological analyses of Ebola virus have revealed that histopathological changes in skin tissue are associated with various degrees of endothelia...

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Autores principales: Hsu, Pei-Chun, Chiou, Bin-Hao, Huang, Chun-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5757418/
https://www.ncbi.nlm.nih.gov/pubmed/29312814
http://dx.doi.org/10.7717/peerj.4138
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author Hsu, Pei-Chun
Chiou, Bin-Hao
Huang, Chun-Ming
author_facet Hsu, Pei-Chun
Chiou, Bin-Hao
Huang, Chun-Ming
author_sort Hsu, Pei-Chun
collection PubMed
description Ebola virus, a negative-sense single-stranded RNA virus, causes severe viral hemorrhagic fever and has a high mortality rate. Histopathological and immunopathological analyses of Ebola virus have revealed that histopathological changes in skin tissue are associated with various degrees of endothelial cell swelling and necrosis. The interactions of microbes within or on a host are a crucial for the skin immune shield. The discovery of microRNAs (miRNAs) in Ebola virus implies that immune escape, endothelial cell rupture, and tissue dissolution during Ebola virus infection are a result of the effects of Ebola virus miRNAs. Keratinocytes obtained from normal skin can attach and spread through expression of the thrombospondin family of proteins, playing a role in initiation of cell-mediated immune responses in the skin. Several miRNAs have been shown to bind the 3′ untranslated region of thrombospondin mRNA, thereby controlling its stability and translational activity. In this study, we discovered short RNA sequences that may act as miRNAs from Propionibacterium acnes using a practical workflow of bioinformatics methods. Subsequently, we deciphered the common target gene. These RNA sequences tended to bind to the same thrombospondin protein, THSD4, emphasizing the potential importance of the synergistic binding of miRNAs from Ebola virus, Propionibacterium acnes, and humans to the target. These results provide important insights into the molecular mechanisms of thrombospondin proteins and miRNAs in Ebola virus infection.
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spelling pubmed-57574182018-01-08 On revealing the gene targets of Ebola virus microRNAs involved in the human skin microbiome Hsu, Pei-Chun Chiou, Bin-Hao Huang, Chun-Ming PeerJ Bioinformatics Ebola virus, a negative-sense single-stranded RNA virus, causes severe viral hemorrhagic fever and has a high mortality rate. Histopathological and immunopathological analyses of Ebola virus have revealed that histopathological changes in skin tissue are associated with various degrees of endothelial cell swelling and necrosis. The interactions of microbes within or on a host are a crucial for the skin immune shield. The discovery of microRNAs (miRNAs) in Ebola virus implies that immune escape, endothelial cell rupture, and tissue dissolution during Ebola virus infection are a result of the effects of Ebola virus miRNAs. Keratinocytes obtained from normal skin can attach and spread through expression of the thrombospondin family of proteins, playing a role in initiation of cell-mediated immune responses in the skin. Several miRNAs have been shown to bind the 3′ untranslated region of thrombospondin mRNA, thereby controlling its stability and translational activity. In this study, we discovered short RNA sequences that may act as miRNAs from Propionibacterium acnes using a practical workflow of bioinformatics methods. Subsequently, we deciphered the common target gene. These RNA sequences tended to bind to the same thrombospondin protein, THSD4, emphasizing the potential importance of the synergistic binding of miRNAs from Ebola virus, Propionibacterium acnes, and humans to the target. These results provide important insights into the molecular mechanisms of thrombospondin proteins and miRNAs in Ebola virus infection. PeerJ Inc. 2018-01-05 /pmc/articles/PMC5757418/ /pubmed/29312814 http://dx.doi.org/10.7717/peerj.4138 Text en ©2018 Hsu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Bioinformatics
Hsu, Pei-Chun
Chiou, Bin-Hao
Huang, Chun-Ming
On revealing the gene targets of Ebola virus microRNAs involved in the human skin microbiome
title On revealing the gene targets of Ebola virus microRNAs involved in the human skin microbiome
title_full On revealing the gene targets of Ebola virus microRNAs involved in the human skin microbiome
title_fullStr On revealing the gene targets of Ebola virus microRNAs involved in the human skin microbiome
title_full_unstemmed On revealing the gene targets of Ebola virus microRNAs involved in the human skin microbiome
title_short On revealing the gene targets of Ebola virus microRNAs involved in the human skin microbiome
title_sort on revealing the gene targets of ebola virus micrornas involved in the human skin microbiome
topic Bioinformatics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5757418/
https://www.ncbi.nlm.nih.gov/pubmed/29312814
http://dx.doi.org/10.7717/peerj.4138
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