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Multiple Retinal Axons Converge onto Relay Cells in the Adult Mouse Thalamus

Activity-dependent refinement of neural circuits is a fundamental principle of neural development. This process has been well studied at retinogeniculate synapses—synapses that form between retinal ganglion cells (RGCs) and relay cells within the dorsal lateral geniculate nucleus. Physiological stud...

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Autores principales: Hammer, Sarah, Monavarfeshani, Aboozar, Lemon, Tyler, Su, Jianmin, Fox, Michael Andrew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5757867/
https://www.ncbi.nlm.nih.gov/pubmed/26321636
http://dx.doi.org/10.1016/j.celrep.2015.08.003
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author Hammer, Sarah
Monavarfeshani, Aboozar
Lemon, Tyler
Su, Jianmin
Fox, Michael Andrew
author_facet Hammer, Sarah
Monavarfeshani, Aboozar
Lemon, Tyler
Su, Jianmin
Fox, Michael Andrew
author_sort Hammer, Sarah
collection PubMed
description Activity-dependent refinement of neural circuits is a fundamental principle of neural development. This process has been well studied at retinogeniculate synapses—synapses that form between retinal ganglion cells (RGCs) and relay cells within the dorsal lateral geniculate nucleus. Physiological studies suggest that shortly after birth, inputs from ~20 RGCs converge onto relay cells. Subsequently, all but just one to two of these inputs are eliminated. Despite widespread acceptance, this notion is at odds with ultrastructural studies showing numerous retinal terminals clustering onto relay cell dendrites in the adult. Here, we explored this discrepancy using brainbow AAVs and serial block face scanning electron microscopy (SBFSEM). Results with both approaches demonstrate that terminals from numerous RGCs cluster onto relay cell dendrites, challenging the notion that only one to two RGCs innervate each relay cell. These findings force us to re-evaluate our understanding of subcortical visual circuitry.
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spelling pubmed-57578672018-01-08 Multiple Retinal Axons Converge onto Relay Cells in the Adult Mouse Thalamus Hammer, Sarah Monavarfeshani, Aboozar Lemon, Tyler Su, Jianmin Fox, Michael Andrew Cell Rep Article Activity-dependent refinement of neural circuits is a fundamental principle of neural development. This process has been well studied at retinogeniculate synapses—synapses that form between retinal ganglion cells (RGCs) and relay cells within the dorsal lateral geniculate nucleus. Physiological studies suggest that shortly after birth, inputs from ~20 RGCs converge onto relay cells. Subsequently, all but just one to two of these inputs are eliminated. Despite widespread acceptance, this notion is at odds with ultrastructural studies showing numerous retinal terminals clustering onto relay cell dendrites in the adult. Here, we explored this discrepancy using brainbow AAVs and serial block face scanning electron microscopy (SBFSEM). Results with both approaches demonstrate that terminals from numerous RGCs cluster onto relay cell dendrites, challenging the notion that only one to two RGCs innervate each relay cell. These findings force us to re-evaluate our understanding of subcortical visual circuitry. 2015-08-28 2015-09-08 /pmc/articles/PMC5757867/ /pubmed/26321636 http://dx.doi.org/10.1016/j.celrep.2015.08.003 Text en http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hammer, Sarah
Monavarfeshani, Aboozar
Lemon, Tyler
Su, Jianmin
Fox, Michael Andrew
Multiple Retinal Axons Converge onto Relay Cells in the Adult Mouse Thalamus
title Multiple Retinal Axons Converge onto Relay Cells in the Adult Mouse Thalamus
title_full Multiple Retinal Axons Converge onto Relay Cells in the Adult Mouse Thalamus
title_fullStr Multiple Retinal Axons Converge onto Relay Cells in the Adult Mouse Thalamus
title_full_unstemmed Multiple Retinal Axons Converge onto Relay Cells in the Adult Mouse Thalamus
title_short Multiple Retinal Axons Converge onto Relay Cells in the Adult Mouse Thalamus
title_sort multiple retinal axons converge onto relay cells in the adult mouse thalamus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5757867/
https://www.ncbi.nlm.nih.gov/pubmed/26321636
http://dx.doi.org/10.1016/j.celrep.2015.08.003
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