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Antioxidant and Cytoprotective Activities of Enzymatic Extracts from Rhizoid of Laminaria japonica

Rhizoid of Laminaria japonica was hydrolyzed with proteases and carbohydrases to obtain antioxidant materials. Oxygen radical absorbance capacity (ORAC) of the enzymatic extracts was evaluated and the Protamex extract (PE) exhibited the highest ORAC value. PE also potently scavenged 2,2-diphenyl-1-p...

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Autores principales: Je, Jae-Young, Park, Soo Yeon, Ahn, Chang-Bum
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Food Science and Nutrition 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5758095/
https://www.ncbi.nlm.nih.gov/pubmed/29333384
http://dx.doi.org/10.3746/pnf.2017.22.4.312
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author Je, Jae-Young
Park, Soo Yeon
Ahn, Chang-Bum
author_facet Je, Jae-Young
Park, Soo Yeon
Ahn, Chang-Bum
author_sort Je, Jae-Young
collection PubMed
description Rhizoid of Laminaria japonica was hydrolyzed with proteases and carbohydrases to obtain antioxidant materials. Oxygen radical absorbance capacity (ORAC) of the enzymatic extracts was evaluated and the Protamex extract (PE) exhibited the highest ORAC value. PE also potently scavenged 2,2-diphenyl-1-picrylhydrazyl radical, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic) acid cation radical, and hydrogen peroxide (H(2)O(2)) and had good reducing power. PE inhibited hydroxyl radical-induced DNA scission by measuring the conversion of supercoiled pBR322 plasmid DNA to the open circular form. The cytoprotective effect of PE against H(2)O(2)-induced hepatic cell damage was also investigated. PE showed a dose-dependent cytoprotective effect in cultured hepatocytes by inhibiting intracellular reactive oxygen species scavenging activity. In addition, PE up-regulated the expression of heme oxygenase-1, which is a cytoprotective enzyme, by activating translocation of nuclear factor-erythroid 2-related factor 2. Taken together, the enzymatic extract of rhizoid of L. japonica, particularly PE, may be useful for antioxidant additives.
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spelling pubmed-57580952018-01-12 Antioxidant and Cytoprotective Activities of Enzymatic Extracts from Rhizoid of Laminaria japonica Je, Jae-Young Park, Soo Yeon Ahn, Chang-Bum Prev Nutr Food Sci Articles Rhizoid of Laminaria japonica was hydrolyzed with proteases and carbohydrases to obtain antioxidant materials. Oxygen radical absorbance capacity (ORAC) of the enzymatic extracts was evaluated and the Protamex extract (PE) exhibited the highest ORAC value. PE also potently scavenged 2,2-diphenyl-1-picrylhydrazyl radical, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic) acid cation radical, and hydrogen peroxide (H(2)O(2)) and had good reducing power. PE inhibited hydroxyl radical-induced DNA scission by measuring the conversion of supercoiled pBR322 plasmid DNA to the open circular form. The cytoprotective effect of PE against H(2)O(2)-induced hepatic cell damage was also investigated. PE showed a dose-dependent cytoprotective effect in cultured hepatocytes by inhibiting intracellular reactive oxygen species scavenging activity. In addition, PE up-regulated the expression of heme oxygenase-1, which is a cytoprotective enzyme, by activating translocation of nuclear factor-erythroid 2-related factor 2. Taken together, the enzymatic extract of rhizoid of L. japonica, particularly PE, may be useful for antioxidant additives. The Korean Society of Food Science and Nutrition 2017-12 2017-12-31 /pmc/articles/PMC5758095/ /pubmed/29333384 http://dx.doi.org/10.3746/pnf.2017.22.4.312 Text en Copyright © 2017 by The Korean Society of Food Science and Nutrition This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Je, Jae-Young
Park, Soo Yeon
Ahn, Chang-Bum
Antioxidant and Cytoprotective Activities of Enzymatic Extracts from Rhizoid of Laminaria japonica
title Antioxidant and Cytoprotective Activities of Enzymatic Extracts from Rhizoid of Laminaria japonica
title_full Antioxidant and Cytoprotective Activities of Enzymatic Extracts from Rhizoid of Laminaria japonica
title_fullStr Antioxidant and Cytoprotective Activities of Enzymatic Extracts from Rhizoid of Laminaria japonica
title_full_unstemmed Antioxidant and Cytoprotective Activities of Enzymatic Extracts from Rhizoid of Laminaria japonica
title_short Antioxidant and Cytoprotective Activities of Enzymatic Extracts from Rhizoid of Laminaria japonica
title_sort antioxidant and cytoprotective activities of enzymatic extracts from rhizoid of laminaria japonica
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5758095/
https://www.ncbi.nlm.nih.gov/pubmed/29333384
http://dx.doi.org/10.3746/pnf.2017.22.4.312
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