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Gas Chromatography, GC/Mass Analysis and Bioactivity of Essential Oil from Aerial Parts of Ferulago trifida: Antimicrobial, Antioxidant, AChE Inhibitory, General Toxicity, MTT Assay and Larvicidal Activities

BACKGROUND: We aimed to investigate different biological properties of aerial parts essential oil of Ferulago trifida Boiss and larvicidal activity of its volatile oils from all parts of plant. METHODS: Essential oil was prepared by steam distillation and analyzed by Gas chromatography and GC/Mass....

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Autores principales: Tavakoli, Saeed, Vatandoost, Hassan, Zeidabadinezhad, Reza, Hajiaghaee, Reza, Hadjiakhoondi, Abbas, Abai, Mohammad Reza, Yassa, Narguess
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5758637/
https://www.ncbi.nlm.nih.gov/pubmed/29322058
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author Tavakoli, Saeed
Vatandoost, Hassan
Zeidabadinezhad, Reza
Hajiaghaee, Reza
Hadjiakhoondi, Abbas
Abai, Mohammad Reza
Yassa, Narguess
author_facet Tavakoli, Saeed
Vatandoost, Hassan
Zeidabadinezhad, Reza
Hajiaghaee, Reza
Hadjiakhoondi, Abbas
Abai, Mohammad Reza
Yassa, Narguess
author_sort Tavakoli, Saeed
collection PubMed
description BACKGROUND: We aimed to investigate different biological properties of aerial parts essential oil of Ferulago trifida Boiss and larvicidal activity of its volatile oils from all parts of plant. METHODS: Essential oil was prepared by steam distillation and analyzed by Gas chromatography and GC/Mass. Antioxidant, antimicrobial, cytotoxic effects and AChE inhibitory of the oil were investigated using DPPH, disk diffusion method, MTT assay and Ellman methods. Larvicidal activity of F. trifida essential oil against malaria vector Anopheles stephensi was carried out according to the method described by WHO. RESULTS: In GC and GC/MS analysis, 58 compounds were identified in the aerial parts essential oil, of which E-verbenol (9.66%), isobutyl acetate (25.73%) and E-β-caryophyllene (8.68%) were main compounds. The oil showed (IC(50)= 111.2μg/ml) in DPPH and IC(50)= 21.5 mg/ml in the investigation of AChE inhibitory. Furthermore, the oil demonstrated toxicity with (LD(50)= 1.1μg/ml) in brine shrimp lethality test and with (IC(50)= 22.0, 25.0 and 42.55 μg/ml) on three cancerous cell lines (MCF-7, A-549 and HT-29) respectively. LC(50) of stem, root, aerial parts, fruits, and flowers essential oils against larvae of An. stephensi were equal with 10.46, 22.27, 20.50, 31.93 and 79.87ppm respectively. In antimicrobial activities, essential oil was effective on all specimens except Escherichia coli, Aspergillus niger and Candida albicans. CONCLUSION: The essential oil showed moderate antioxidant activity, strong antimicrobial properties and good toxic effect in brine shrimp test and MTT assay on three cancerous cell lines.
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spelling pubmed-57586372018-01-10 Gas Chromatography, GC/Mass Analysis and Bioactivity of Essential Oil from Aerial Parts of Ferulago trifida: Antimicrobial, Antioxidant, AChE Inhibitory, General Toxicity, MTT Assay and Larvicidal Activities Tavakoli, Saeed Vatandoost, Hassan Zeidabadinezhad, Reza Hajiaghaee, Reza Hadjiakhoondi, Abbas Abai, Mohammad Reza Yassa, Narguess J Arthropod Borne Dis Original Article BACKGROUND: We aimed to investigate different biological properties of aerial parts essential oil of Ferulago trifida Boiss and larvicidal activity of its volatile oils from all parts of plant. METHODS: Essential oil was prepared by steam distillation and analyzed by Gas chromatography and GC/Mass. Antioxidant, antimicrobial, cytotoxic effects and AChE inhibitory of the oil were investigated using DPPH, disk diffusion method, MTT assay and Ellman methods. Larvicidal activity of F. trifida essential oil against malaria vector Anopheles stephensi was carried out according to the method described by WHO. RESULTS: In GC and GC/MS analysis, 58 compounds were identified in the aerial parts essential oil, of which E-verbenol (9.66%), isobutyl acetate (25.73%) and E-β-caryophyllene (8.68%) were main compounds. The oil showed (IC(50)= 111.2μg/ml) in DPPH and IC(50)= 21.5 mg/ml in the investigation of AChE inhibitory. Furthermore, the oil demonstrated toxicity with (LD(50)= 1.1μg/ml) in brine shrimp lethality test and with (IC(50)= 22.0, 25.0 and 42.55 μg/ml) on three cancerous cell lines (MCF-7, A-549 and HT-29) respectively. LC(50) of stem, root, aerial parts, fruits, and flowers essential oils against larvae of An. stephensi were equal with 10.46, 22.27, 20.50, 31.93 and 79.87ppm respectively. In antimicrobial activities, essential oil was effective on all specimens except Escherichia coli, Aspergillus niger and Candida albicans. CONCLUSION: The essential oil showed moderate antioxidant activity, strong antimicrobial properties and good toxic effect in brine shrimp test and MTT assay on three cancerous cell lines. Tehran University of Medical Sciences 2017-09-08 /pmc/articles/PMC5758637/ /pubmed/29322058 Text en Copyright© Iranian Society of Medical Entomology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Tavakoli, Saeed
Vatandoost, Hassan
Zeidabadinezhad, Reza
Hajiaghaee, Reza
Hadjiakhoondi, Abbas
Abai, Mohammad Reza
Yassa, Narguess
Gas Chromatography, GC/Mass Analysis and Bioactivity of Essential Oil from Aerial Parts of Ferulago trifida: Antimicrobial, Antioxidant, AChE Inhibitory, General Toxicity, MTT Assay and Larvicidal Activities
title Gas Chromatography, GC/Mass Analysis and Bioactivity of Essential Oil from Aerial Parts of Ferulago trifida: Antimicrobial, Antioxidant, AChE Inhibitory, General Toxicity, MTT Assay and Larvicidal Activities
title_full Gas Chromatography, GC/Mass Analysis and Bioactivity of Essential Oil from Aerial Parts of Ferulago trifida: Antimicrobial, Antioxidant, AChE Inhibitory, General Toxicity, MTT Assay and Larvicidal Activities
title_fullStr Gas Chromatography, GC/Mass Analysis and Bioactivity of Essential Oil from Aerial Parts of Ferulago trifida: Antimicrobial, Antioxidant, AChE Inhibitory, General Toxicity, MTT Assay and Larvicidal Activities
title_full_unstemmed Gas Chromatography, GC/Mass Analysis and Bioactivity of Essential Oil from Aerial Parts of Ferulago trifida: Antimicrobial, Antioxidant, AChE Inhibitory, General Toxicity, MTT Assay and Larvicidal Activities
title_short Gas Chromatography, GC/Mass Analysis and Bioactivity of Essential Oil from Aerial Parts of Ferulago trifida: Antimicrobial, Antioxidant, AChE Inhibitory, General Toxicity, MTT Assay and Larvicidal Activities
title_sort gas chromatography, gc/mass analysis and bioactivity of essential oil from aerial parts of ferulago trifida: antimicrobial, antioxidant, ache inhibitory, general toxicity, mtt assay and larvicidal activities
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5758637/
https://www.ncbi.nlm.nih.gov/pubmed/29322058
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