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Anti-glioma Natural Products Downregulating Tumor Glycolytic Enzymes from Marine Actinomycete Streptomyces sp. ZZ406

Marine natural products are important resources for discovering novel anticancer drugs. In this study, an extract prepared from the culture of a sea anemone-derived actinomycete Streptomyces sp. ZZ406 in soluble starch and casein-related liquid medium was found to have activity in inhibiting the pro...

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Autores principales: Chen, Mengxuan, Chai, Weiyun, Song, Tengfei, Ma, Mingzhu, Lian, Xiao-Yuan, Zhang, Zhizhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5758648/
https://www.ncbi.nlm.nih.gov/pubmed/29311676
http://dx.doi.org/10.1038/s41598-017-18484-7
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author Chen, Mengxuan
Chai, Weiyun
Song, Tengfei
Ma, Mingzhu
Lian, Xiao-Yuan
Zhang, Zhizhen
author_facet Chen, Mengxuan
Chai, Weiyun
Song, Tengfei
Ma, Mingzhu
Lian, Xiao-Yuan
Zhang, Zhizhen
author_sort Chen, Mengxuan
collection PubMed
description Marine natural products are important resources for discovering novel anticancer drugs. In this study, an extract prepared from the culture of a sea anemone-derived actinomycete Streptomyces sp. ZZ406 in soluble starch and casein-related liquid medium was found to have activity in inhibiting the proliferation of glioma cells and reducing the production of lactate in glioma cells. Chemical investigation of this active crude extract resulted in the isolation of four new compounds and seven known ones. Structures of the new compounds were determined by a combination of extensive NMR analyses, HRESIMS and MS-MS data, electronic circular dichroism calculation, chemical degradation, and Marfey’s method. New compound 1 showed potent activity against the proliferation of different glioma cells with IC(50) values of 4.7 to 8.1 μM, high selectivity index (>12.3 to 21.3), and good stability in human liver microsomes. Western blot analysis revealed that compound 1 remarkably downregulated the expressions of several important glioma glycolytic enzymes. The data from this study suggested that compound 1 might have potential as a novel anti-glioma agent to be further investigated.
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spelling pubmed-57586482018-01-10 Anti-glioma Natural Products Downregulating Tumor Glycolytic Enzymes from Marine Actinomycete Streptomyces sp. ZZ406 Chen, Mengxuan Chai, Weiyun Song, Tengfei Ma, Mingzhu Lian, Xiao-Yuan Zhang, Zhizhen Sci Rep Article Marine natural products are important resources for discovering novel anticancer drugs. In this study, an extract prepared from the culture of a sea anemone-derived actinomycete Streptomyces sp. ZZ406 in soluble starch and casein-related liquid medium was found to have activity in inhibiting the proliferation of glioma cells and reducing the production of lactate in glioma cells. Chemical investigation of this active crude extract resulted in the isolation of four new compounds and seven known ones. Structures of the new compounds were determined by a combination of extensive NMR analyses, HRESIMS and MS-MS data, electronic circular dichroism calculation, chemical degradation, and Marfey’s method. New compound 1 showed potent activity against the proliferation of different glioma cells with IC(50) values of 4.7 to 8.1 μM, high selectivity index (>12.3 to 21.3), and good stability in human liver microsomes. Western blot analysis revealed that compound 1 remarkably downregulated the expressions of several important glioma glycolytic enzymes. The data from this study suggested that compound 1 might have potential as a novel anti-glioma agent to be further investigated. Nature Publishing Group UK 2018-01-08 /pmc/articles/PMC5758648/ /pubmed/29311676 http://dx.doi.org/10.1038/s41598-017-18484-7 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Chen, Mengxuan
Chai, Weiyun
Song, Tengfei
Ma, Mingzhu
Lian, Xiao-Yuan
Zhang, Zhizhen
Anti-glioma Natural Products Downregulating Tumor Glycolytic Enzymes from Marine Actinomycete Streptomyces sp. ZZ406
title Anti-glioma Natural Products Downregulating Tumor Glycolytic Enzymes from Marine Actinomycete Streptomyces sp. ZZ406
title_full Anti-glioma Natural Products Downregulating Tumor Glycolytic Enzymes from Marine Actinomycete Streptomyces sp. ZZ406
title_fullStr Anti-glioma Natural Products Downregulating Tumor Glycolytic Enzymes from Marine Actinomycete Streptomyces sp. ZZ406
title_full_unstemmed Anti-glioma Natural Products Downregulating Tumor Glycolytic Enzymes from Marine Actinomycete Streptomyces sp. ZZ406
title_short Anti-glioma Natural Products Downregulating Tumor Glycolytic Enzymes from Marine Actinomycete Streptomyces sp. ZZ406
title_sort anti-glioma natural products downregulating tumor glycolytic enzymes from marine actinomycete streptomyces sp. zz406
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5758648/
https://www.ncbi.nlm.nih.gov/pubmed/29311676
http://dx.doi.org/10.1038/s41598-017-18484-7
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