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Segmented cell analyses to measure redox states of autofluorescent NAD(P)H, FAD & Trp in cancer cells by FLIM

Multiphoton FLIM microscopy offers many opportunities to investigate processes in live cells, tissue and animal model systems. For redox measurements, FLIM data is mostly published by cell mean values and intensity-based redox ratios. Our method is based entirely on FLIM parameters generated by 3-de...

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Detalles Bibliográficos
Autores principales:	Wallrabe, Horst, Svindrych, Zdenek, Alam, Shagufta R., Siller, Karsten H., Wang, Tianxiong, Kashatus, David, Hu, Song, Periasamy, Ammasi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5758727/ https://www.ncbi.nlm.nih.gov/pubmed/29311591 http://dx.doi.org/10.1038/s41598-017-18634-x

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