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Interneuron-specific signaling evokes distinctive somatostatin-mediated responses in adult cortical astrocytes
The signaling diversity of GABAergic interneurons to post-synaptic neurons is crucial to generate the functional heterogeneity that characterizes brain circuits. Whether this diversity applies to other brain cells, such as the glial cells astrocytes, remains unexplored. Using optogenetics and two-ph...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5758790/ https://www.ncbi.nlm.nih.gov/pubmed/29311610 http://dx.doi.org/10.1038/s41467-017-02642-6 |
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author | Mariotti, Letizia Losi, Gabriele Lia, Annamaria Melone, Marcello Chiavegato, Angela Gómez-Gonzalo, Marta Sessolo, Michele Bovetti, Serena Forli, Angelo Zonta, Micaela Requie, Linda Maria Marcon, Iacopo Pugliese, Arianna Viollet, Cécile Bettler, Bernhard Fellin, Tommaso Conti, Fiorenzo Carmignoto, Giorgio |
author_facet | Mariotti, Letizia Losi, Gabriele Lia, Annamaria Melone, Marcello Chiavegato, Angela Gómez-Gonzalo, Marta Sessolo, Michele Bovetti, Serena Forli, Angelo Zonta, Micaela Requie, Linda Maria Marcon, Iacopo Pugliese, Arianna Viollet, Cécile Bettler, Bernhard Fellin, Tommaso Conti, Fiorenzo Carmignoto, Giorgio |
author_sort | Mariotti, Letizia |
collection | PubMed |
description | The signaling diversity of GABAergic interneurons to post-synaptic neurons is crucial to generate the functional heterogeneity that characterizes brain circuits. Whether this diversity applies to other brain cells, such as the glial cells astrocytes, remains unexplored. Using optogenetics and two-photon functional imaging in the adult mouse neocortex, we here reveal that parvalbumin- and somatostatin-expressing interneurons, two key interneuron classes in the brain, differentially signal to astrocytes inducing weak and robust GABA(B) receptor-mediated Ca(2+) elevations, respectively. Furthermore, the astrocyte response depresses upon parvalbumin interneuron repetitive stimulations and potentiates upon somatostatin interneuron repetitive stimulations, revealing a distinguished astrocyte plasticity. Remarkably, the potentiated response crucially depends on the neuropeptide somatostatin, released by somatostatin interneurons, which activates somatostatin receptors at astrocytic processes. Our study unveils, in the living brain, a hitherto unidentified signaling specificity between interneuron subtypes and astrocytes opening a new perspective into the role of astrocytes as non-neuronal components of inhibitory circuits. |
format | Online Article Text |
id | pubmed-5758790 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57587902018-01-12 Interneuron-specific signaling evokes distinctive somatostatin-mediated responses in adult cortical astrocytes Mariotti, Letizia Losi, Gabriele Lia, Annamaria Melone, Marcello Chiavegato, Angela Gómez-Gonzalo, Marta Sessolo, Michele Bovetti, Serena Forli, Angelo Zonta, Micaela Requie, Linda Maria Marcon, Iacopo Pugliese, Arianna Viollet, Cécile Bettler, Bernhard Fellin, Tommaso Conti, Fiorenzo Carmignoto, Giorgio Nat Commun Article The signaling diversity of GABAergic interneurons to post-synaptic neurons is crucial to generate the functional heterogeneity that characterizes brain circuits. Whether this diversity applies to other brain cells, such as the glial cells astrocytes, remains unexplored. Using optogenetics and two-photon functional imaging in the adult mouse neocortex, we here reveal that parvalbumin- and somatostatin-expressing interneurons, two key interneuron classes in the brain, differentially signal to astrocytes inducing weak and robust GABA(B) receptor-mediated Ca(2+) elevations, respectively. Furthermore, the astrocyte response depresses upon parvalbumin interneuron repetitive stimulations and potentiates upon somatostatin interneuron repetitive stimulations, revealing a distinguished astrocyte plasticity. Remarkably, the potentiated response crucially depends on the neuropeptide somatostatin, released by somatostatin interneurons, which activates somatostatin receptors at astrocytic processes. Our study unveils, in the living brain, a hitherto unidentified signaling specificity between interneuron subtypes and astrocytes opening a new perspective into the role of astrocytes as non-neuronal components of inhibitory circuits. Nature Publishing Group UK 2018-01-08 /pmc/articles/PMC5758790/ /pubmed/29311610 http://dx.doi.org/10.1038/s41467-017-02642-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Mariotti, Letizia Losi, Gabriele Lia, Annamaria Melone, Marcello Chiavegato, Angela Gómez-Gonzalo, Marta Sessolo, Michele Bovetti, Serena Forli, Angelo Zonta, Micaela Requie, Linda Maria Marcon, Iacopo Pugliese, Arianna Viollet, Cécile Bettler, Bernhard Fellin, Tommaso Conti, Fiorenzo Carmignoto, Giorgio Interneuron-specific signaling evokes distinctive somatostatin-mediated responses in adult cortical astrocytes |
title | Interneuron-specific signaling evokes distinctive somatostatin-mediated responses in adult cortical astrocytes |
title_full | Interneuron-specific signaling evokes distinctive somatostatin-mediated responses in adult cortical astrocytes |
title_fullStr | Interneuron-specific signaling evokes distinctive somatostatin-mediated responses in adult cortical astrocytes |
title_full_unstemmed | Interneuron-specific signaling evokes distinctive somatostatin-mediated responses in adult cortical astrocytes |
title_short | Interneuron-specific signaling evokes distinctive somatostatin-mediated responses in adult cortical astrocytes |
title_sort | interneuron-specific signaling evokes distinctive somatostatin-mediated responses in adult cortical astrocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5758790/ https://www.ncbi.nlm.nih.gov/pubmed/29311610 http://dx.doi.org/10.1038/s41467-017-02642-6 |
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