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Preclinical longitudinal imaging of tumor microvascular radiobiological response with functional optical coherence tomography

Radiation therapy (RT) is widely used for cancer treatment, alone or in combination with other therapies. Recent RT advances have revived interest in delivering higher dose in fewer fractions, which may invoke both cellular and microvascular damage mechanisms. Microvasculature may thus be a potentia...

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Autores principales: Demidov, Valentin, Maeda, Azusa, Sugita, Mitsuro, Madge, Victoria, Sadanand, Siddharth, Flueraru, Costel, Vitkin, I. Alex
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5758802/
https://www.ncbi.nlm.nih.gov/pubmed/29311686
http://dx.doi.org/10.1038/s41598-017-18635-w
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author Demidov, Valentin
Maeda, Azusa
Sugita, Mitsuro
Madge, Victoria
Sadanand, Siddharth
Flueraru, Costel
Vitkin, I. Alex
author_facet Demidov, Valentin
Maeda, Azusa
Sugita, Mitsuro
Madge, Victoria
Sadanand, Siddharth
Flueraru, Costel
Vitkin, I. Alex
author_sort Demidov, Valentin
collection PubMed
description Radiation therapy (RT) is widely used for cancer treatment, alone or in combination with other therapies. Recent RT advances have revived interest in delivering higher dose in fewer fractions, which may invoke both cellular and microvascular damage mechanisms. Microvasculature may thus be a potentially sensitive functional biomarker of RT early response, especially for such emerging RT treatments. However it is difficult to measure directly and non-invasively, and its time course, dose dependencies, and overall importance in tumor control are unclear. We use functional optical coherence tomography for quantitative longitudinal in vivo imaging in preclinical models of human tumor xenografts subjected to 10, 20 and 30 Gy doses, furnishing a detailed assessment of vascular remodeling following RT. Immediate (minutes to tens of minutes) and early (days to weeks) RT responses of microvascular supply, as well as tumor volume and fluorescence intensity, were quantified and demonstrated robust and complex temporal dose-dependent behaviors. The findings were compared to theoretical models proposed in the literature.
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spelling pubmed-57588022018-01-10 Preclinical longitudinal imaging of tumor microvascular radiobiological response with functional optical coherence tomography Demidov, Valentin Maeda, Azusa Sugita, Mitsuro Madge, Victoria Sadanand, Siddharth Flueraru, Costel Vitkin, I. Alex Sci Rep Article Radiation therapy (RT) is widely used for cancer treatment, alone or in combination with other therapies. Recent RT advances have revived interest in delivering higher dose in fewer fractions, which may invoke both cellular and microvascular damage mechanisms. Microvasculature may thus be a potentially sensitive functional biomarker of RT early response, especially for such emerging RT treatments. However it is difficult to measure directly and non-invasively, and its time course, dose dependencies, and overall importance in tumor control are unclear. We use functional optical coherence tomography for quantitative longitudinal in vivo imaging in preclinical models of human tumor xenografts subjected to 10, 20 and 30 Gy doses, furnishing a detailed assessment of vascular remodeling following RT. Immediate (minutes to tens of minutes) and early (days to weeks) RT responses of microvascular supply, as well as tumor volume and fluorescence intensity, were quantified and demonstrated robust and complex temporal dose-dependent behaviors. The findings were compared to theoretical models proposed in the literature. Nature Publishing Group UK 2018-01-08 /pmc/articles/PMC5758802/ /pubmed/29311686 http://dx.doi.org/10.1038/s41598-017-18635-w Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Demidov, Valentin
Maeda, Azusa
Sugita, Mitsuro
Madge, Victoria
Sadanand, Siddharth
Flueraru, Costel
Vitkin, I. Alex
Preclinical longitudinal imaging of tumor microvascular radiobiological response with functional optical coherence tomography
title Preclinical longitudinal imaging of tumor microvascular radiobiological response with functional optical coherence tomography
title_full Preclinical longitudinal imaging of tumor microvascular radiobiological response with functional optical coherence tomography
title_fullStr Preclinical longitudinal imaging of tumor microvascular radiobiological response with functional optical coherence tomography
title_full_unstemmed Preclinical longitudinal imaging of tumor microvascular radiobiological response with functional optical coherence tomography
title_short Preclinical longitudinal imaging of tumor microvascular radiobiological response with functional optical coherence tomography
title_sort preclinical longitudinal imaging of tumor microvascular radiobiological response with functional optical coherence tomography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5758802/
https://www.ncbi.nlm.nih.gov/pubmed/29311686
http://dx.doi.org/10.1038/s41598-017-18635-w
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