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Construction of an ultrahigh-density genetic linkage map for Jatropha curcas L. and identification of QTL for fruit yield
BACKGROUND: As an important biofuel plant, the demand for higher yield Jatropha curcas L. is rapidly increasing. However, genetic analysis of Jatropha and molecular breeding for higher yield have been hampered by the limited number of molecular markers available. RESULTS: An ultrahigh-density linkag...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5759280/ https://www.ncbi.nlm.nih.gov/pubmed/29321812 http://dx.doi.org/10.1186/s13068-017-1004-9 |
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author | Xia, Zhiqiang Zhang, Shengkui Wen, Mingfu Lu, Cheng Sun, Yufang Zou, Meiling Wang, Wenquan |
author_facet | Xia, Zhiqiang Zhang, Shengkui Wen, Mingfu Lu, Cheng Sun, Yufang Zou, Meiling Wang, Wenquan |
author_sort | Xia, Zhiqiang |
collection | PubMed |
description | BACKGROUND: As an important biofuel plant, the demand for higher yield Jatropha curcas L. is rapidly increasing. However, genetic analysis of Jatropha and molecular breeding for higher yield have been hampered by the limited number of molecular markers available. RESULTS: An ultrahigh-density linkage map for a Jatropha mapping population of 153 individuals was constructed and covered 1380.58 cM of the Jatropha genome, with average marker density of 0.403 cM. The genetic linkage map consisted of 3422 SNP and indel markers, which clustered into 11 linkage groups. With this map, 13 repeatable QTLs (reQTLs) for fruit yield traits were identified. Ten reQTLs, qNF-1, qNF-2a, qNF-2b, qNF-2c, qNF-3, qNF-4, qNF-6, qNF-7a, qNF-7b and qNF-8, that control the number of fruits (NF) mapped to LGs 1, 2, 3, 4, 6, 7 and 8, whereas three reQTLs, qTWF-1, qTWF-2 and qTWF-3, that control the total weight of fruits (TWF) mapped to LGs 1, 2 and 3, respectively. It is interesting that there are two candidate critical genes, which may regulate Jatropha fruit yield. We also identified three pleiotropic reQTL pairs associated with both the NF and TWF traits. CONCLUSION: This study is the first to report an ultrahigh-density Jatropha genetic linkage map construction, and the markers used in this study showed great potential for QTL mapping. Thirteen fruit-yield reQTLs and two important candidate genes were identified based on this linkage map. This genetic linkage map will be a useful tool for the localization of other economically important QTLs and candidate genes for Jatropha. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13068-017-1004-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5759280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57592802018-01-10 Construction of an ultrahigh-density genetic linkage map for Jatropha curcas L. and identification of QTL for fruit yield Xia, Zhiqiang Zhang, Shengkui Wen, Mingfu Lu, Cheng Sun, Yufang Zou, Meiling Wang, Wenquan Biotechnol Biofuels Research BACKGROUND: As an important biofuel plant, the demand for higher yield Jatropha curcas L. is rapidly increasing. However, genetic analysis of Jatropha and molecular breeding for higher yield have been hampered by the limited number of molecular markers available. RESULTS: An ultrahigh-density linkage map for a Jatropha mapping population of 153 individuals was constructed and covered 1380.58 cM of the Jatropha genome, with average marker density of 0.403 cM. The genetic linkage map consisted of 3422 SNP and indel markers, which clustered into 11 linkage groups. With this map, 13 repeatable QTLs (reQTLs) for fruit yield traits were identified. Ten reQTLs, qNF-1, qNF-2a, qNF-2b, qNF-2c, qNF-3, qNF-4, qNF-6, qNF-7a, qNF-7b and qNF-8, that control the number of fruits (NF) mapped to LGs 1, 2, 3, 4, 6, 7 and 8, whereas three reQTLs, qTWF-1, qTWF-2 and qTWF-3, that control the total weight of fruits (TWF) mapped to LGs 1, 2 and 3, respectively. It is interesting that there are two candidate critical genes, which may regulate Jatropha fruit yield. We also identified three pleiotropic reQTL pairs associated with both the NF and TWF traits. CONCLUSION: This study is the first to report an ultrahigh-density Jatropha genetic linkage map construction, and the markers used in this study showed great potential for QTL mapping. Thirteen fruit-yield reQTLs and two important candidate genes were identified based on this linkage map. This genetic linkage map will be a useful tool for the localization of other economically important QTLs and candidate genes for Jatropha. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13068-017-1004-9) contains supplementary material, which is available to authorized users. BioMed Central 2018-01-09 /pmc/articles/PMC5759280/ /pubmed/29321812 http://dx.doi.org/10.1186/s13068-017-1004-9 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Xia, Zhiqiang Zhang, Shengkui Wen, Mingfu Lu, Cheng Sun, Yufang Zou, Meiling Wang, Wenquan Construction of an ultrahigh-density genetic linkage map for Jatropha curcas L. and identification of QTL for fruit yield |
title | Construction of an ultrahigh-density genetic linkage map for Jatropha curcas L. and identification of QTL for fruit yield |
title_full | Construction of an ultrahigh-density genetic linkage map for Jatropha curcas L. and identification of QTL for fruit yield |
title_fullStr | Construction of an ultrahigh-density genetic linkage map for Jatropha curcas L. and identification of QTL for fruit yield |
title_full_unstemmed | Construction of an ultrahigh-density genetic linkage map for Jatropha curcas L. and identification of QTL for fruit yield |
title_short | Construction of an ultrahigh-density genetic linkage map for Jatropha curcas L. and identification of QTL for fruit yield |
title_sort | construction of an ultrahigh-density genetic linkage map for jatropha curcas l. and identification of qtl for fruit yield |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5759280/ https://www.ncbi.nlm.nih.gov/pubmed/29321812 http://dx.doi.org/10.1186/s13068-017-1004-9 |
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