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Transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlings

BACKGROUND: Breeding rubber tree seedling with growth heterosis is vital for natural rubber production. It is the prerequisites for effectively utilizing growth heterosis to elucidate its molecular mechanisms, but the molecular mechanisms remain poorly understood in rubber tree. To elucidate seedlin...

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Autores principales: Yang, Hong, Wang, Xuncheng, Wei, Yongxuan, Deng, Zhi, Liu, Hui, Chen, Jiangshu, Dai, Longjun, Xia, Zhihui, He, Guangming, Li, Dejun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5759766/
https://www.ncbi.nlm.nih.gov/pubmed/29316882
http://dx.doi.org/10.1186/s12870-017-1203-3
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author Yang, Hong
Wang, Xuncheng
Wei, Yongxuan
Deng, Zhi
Liu, Hui
Chen, Jiangshu
Dai, Longjun
Xia, Zhihui
He, Guangming
Li, Dejun
author_facet Yang, Hong
Wang, Xuncheng
Wei, Yongxuan
Deng, Zhi
Liu, Hui
Chen, Jiangshu
Dai, Longjun
Xia, Zhihui
He, Guangming
Li, Dejun
author_sort Yang, Hong
collection PubMed
description BACKGROUND: Breeding rubber tree seedling with growth heterosis is vital for natural rubber production. It is the prerequisites for effectively utilizing growth heterosis to elucidate its molecular mechanisms, but the molecular mechanisms remain poorly understood in rubber tree. To elucidate seedling growth heterosis, we conducted comparative transcriptomic analyses between the two hybrids and their parents. RESULTS: By identifying and comparing differently expressed genes (DEGs), we found that the hybrids (BT 3410 and WC 11) show significantly differential expression profiles from their parents (PR 107 and RRIM 600). In BT 3410-parent triad, 1092 (49.95%) and 1094 (50.05%) DEGs indicated clear underdominance or overdominance, respectively. Whereas in WC 11-parent triad, most DEGs (78.2%, 721) showed low- or high-parent dominance; 160 (17.35%) exhibited expression patterns that are not statistically distinguishable from additivity, and 8 (0.87%) and 33 (3.58%) DEGs exhibited underdominance and overdominance, respectively. Furthermore, some biological processes are differentially regulated between two hybrids. Interestingly, the pathway in response to stimulus is significantly downregulated and metabolic pathways are more highly regulated in BT 3410. CONCLUSIONS: Taken together, the genotypes, transcriptomes and biological pathways (especially, carbohydrate metabolism) are highly divergent between two hybrids, which may be associated with growth heterosis and weakness. Analyzing gene action models in hybrid-parent triads, we propose that overdominance may play important roles on growth heterosis, whereas dominance on hybrid weakness in rubber tree seedlings. These findings bring new insights into our understanding of growth heterosis of rubber tree seedling. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi: 10.1186/s12870-017-1203-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-57597662018-01-16 Transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlings Yang, Hong Wang, Xuncheng Wei, Yongxuan Deng, Zhi Liu, Hui Chen, Jiangshu Dai, Longjun Xia, Zhihui He, Guangming Li, Dejun BMC Plant Biol Research Article BACKGROUND: Breeding rubber tree seedling with growth heterosis is vital for natural rubber production. It is the prerequisites for effectively utilizing growth heterosis to elucidate its molecular mechanisms, but the molecular mechanisms remain poorly understood in rubber tree. To elucidate seedling growth heterosis, we conducted comparative transcriptomic analyses between the two hybrids and their parents. RESULTS: By identifying and comparing differently expressed genes (DEGs), we found that the hybrids (BT 3410 and WC 11) show significantly differential expression profiles from their parents (PR 107 and RRIM 600). In BT 3410-parent triad, 1092 (49.95%) and 1094 (50.05%) DEGs indicated clear underdominance or overdominance, respectively. Whereas in WC 11-parent triad, most DEGs (78.2%, 721) showed low- or high-parent dominance; 160 (17.35%) exhibited expression patterns that are not statistically distinguishable from additivity, and 8 (0.87%) and 33 (3.58%) DEGs exhibited underdominance and overdominance, respectively. Furthermore, some biological processes are differentially regulated between two hybrids. Interestingly, the pathway in response to stimulus is significantly downregulated and metabolic pathways are more highly regulated in BT 3410. CONCLUSIONS: Taken together, the genotypes, transcriptomes and biological pathways (especially, carbohydrate metabolism) are highly divergent between two hybrids, which may be associated with growth heterosis and weakness. Analyzing gene action models in hybrid-parent triads, we propose that overdominance may play important roles on growth heterosis, whereas dominance on hybrid weakness in rubber tree seedlings. These findings bring new insights into our understanding of growth heterosis of rubber tree seedling. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi: 10.1186/s12870-017-1203-3) contains supplementary material, which is available to authorized users. BioMed Central 2018-01-09 /pmc/articles/PMC5759766/ /pubmed/29316882 http://dx.doi.org/10.1186/s12870-017-1203-3 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Yang, Hong
Wang, Xuncheng
Wei, Yongxuan
Deng, Zhi
Liu, Hui
Chen, Jiangshu
Dai, Longjun
Xia, Zhihui
He, Guangming
Li, Dejun
Transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlings
title Transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlings
title_full Transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlings
title_fullStr Transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlings
title_full_unstemmed Transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlings
title_short Transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlings
title_sort transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlings
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5759766/
https://www.ncbi.nlm.nih.gov/pubmed/29316882
http://dx.doi.org/10.1186/s12870-017-1203-3
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