Chemical biology of genomic DNA: minimizing PCR bias†

The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde...

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Detalles Bibliográficos
Autores principales: McInroy, Gordon R., Raiber, Eun-Ang, Balasubramanian, Shankar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5759930/
https://www.ncbi.nlm.nih.gov/pubmed/25178041
http://dx.doi.org/10.1039/c4cc05107f
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author McInroy, Gordon R.
Raiber, Eun-Ang
Balasubramanian, Shankar
author_facet McInroy, Gordon R.
Raiber, Eun-Ang
Balasubramanian, Shankar
author_sort McInroy, Gordon R.
collection PubMed
description The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde-reactive biotinylation probe.
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spelling pubmed-57599302018-01-09 Chemical biology of genomic DNA: minimizing PCR bias† McInroy, Gordon R. Raiber, Eun-Ang Balasubramanian, Shankar Chem Commun (Camb) Article The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde-reactive biotinylation probe. 2014-10-18 /pmc/articles/PMC5759930/ /pubmed/25178041 http://dx.doi.org/10.1039/c4cc05107f Text en http://creativecommons.org/licenses/by/3.0/ This article is licensed under a Creative Commons Attribution 3.0 (http://creativecommons.org/licenses/by/3.0/) Unported Licence.
spellingShingle Article
McInroy, Gordon R.
Raiber, Eun-Ang
Balasubramanian, Shankar
Chemical biology of genomic DNA: minimizing PCR bias†
title Chemical biology of genomic DNA: minimizing PCR bias†
title_full Chemical biology of genomic DNA: minimizing PCR bias†
title_fullStr Chemical biology of genomic DNA: minimizing PCR bias†
title_full_unstemmed Chemical biology of genomic DNA: minimizing PCR bias†
title_short Chemical biology of genomic DNA: minimizing PCR bias†
title_sort chemical biology of genomic dna: minimizing pcr bias†
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5759930/
https://www.ncbi.nlm.nih.gov/pubmed/25178041
http://dx.doi.org/10.1039/c4cc05107f
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AT balasubramanianshankar chemicalbiologyofgenomicdnaminimizingpcrbias

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