Cargando…

Comparison of changes in mitochondrial bioenergetics between keratinocytes in human external auditory canal skin and cholesteatomas from normoxia to hypoxia

Cholesteatoma has attracted many studies seeking to uncover its nature and the pathogenesis of related diseases. However, no researchers have explored the mitochondrial bioenergetics of cholesteatoma. The aim of this study was to investigate the energy demand and differential mitochondrial respirati...

Descripción completa

Detalles Bibliográficos
Autores principales: Shih, Cheng-Ping, Lee, Jen-Tin, Chen, Hang-Kang, Lin, Yi-Chun, Chen, Hsin-Chien, Lin, Yuan-Yung, Kuo, Chao-Yin, Chen, Yu-Ting, Wang, Chih-Hung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760563/
https://www.ncbi.nlm.nih.gov/pubmed/29317713
http://dx.doi.org/10.1038/s41598-017-18536-y
_version_ 1783291382187687936
author Shih, Cheng-Ping
Lee, Jen-Tin
Chen, Hang-Kang
Lin, Yi-Chun
Chen, Hsin-Chien
Lin, Yuan-Yung
Kuo, Chao-Yin
Chen, Yu-Ting
Wang, Chih-Hung
author_facet Shih, Cheng-Ping
Lee, Jen-Tin
Chen, Hang-Kang
Lin, Yi-Chun
Chen, Hsin-Chien
Lin, Yuan-Yung
Kuo, Chao-Yin
Chen, Yu-Ting
Wang, Chih-Hung
author_sort Shih, Cheng-Ping
collection PubMed
description Cholesteatoma has attracted many studies seeking to uncover its nature and the pathogenesis of related diseases. However, no researchers have explored the mitochondrial bioenergetics of cholesteatoma. The aim of this study was to investigate the energy demand and differential mitochondrial respiration profiles between keratinocytes in external auditory canal (EAC) skin and cholesteatoma samples cultured in normoxic (20% O(2)) and hypoxic (5% O(2)) conditions. Enhanced cellular proliferation of both types of keratinocytes was found in hypoxia compared to normoxia. In 20% O(2) conditions, cholesteatoma keratinocytes exhibited less mitochondrial mass, lower ATP levels, and significantly lower basal oxygen consumption rate (OCR) and reserve capacity compared to normal skin keratinocytes. In contrast, in hypoxic conditions, cholesteatoma keratinocytes showed markedly higher levels in maximal OCR and reserve capacity, as well as lower proton leak OCRs, compared to normal skin keratinocytes. Hypoxia induced the reverse mitochondrial bioenergy profile from that in normoxia between these two types of keratinocytes, implying that an adaptive change of mitochondrial respiration to oxygen fluctuations may develop in cases of cholesteatoma. Such adaptation in response to hypoxic conditions may play a role in explaining the pathogenesis of acquired cholesteatoma.
format Online
Article
Text
id pubmed-5760563
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-57605632018-01-17 Comparison of changes in mitochondrial bioenergetics between keratinocytes in human external auditory canal skin and cholesteatomas from normoxia to hypoxia Shih, Cheng-Ping Lee, Jen-Tin Chen, Hang-Kang Lin, Yi-Chun Chen, Hsin-Chien Lin, Yuan-Yung Kuo, Chao-Yin Chen, Yu-Ting Wang, Chih-Hung Sci Rep Article Cholesteatoma has attracted many studies seeking to uncover its nature and the pathogenesis of related diseases. However, no researchers have explored the mitochondrial bioenergetics of cholesteatoma. The aim of this study was to investigate the energy demand and differential mitochondrial respiration profiles between keratinocytes in external auditory canal (EAC) skin and cholesteatoma samples cultured in normoxic (20% O(2)) and hypoxic (5% O(2)) conditions. Enhanced cellular proliferation of both types of keratinocytes was found in hypoxia compared to normoxia. In 20% O(2) conditions, cholesteatoma keratinocytes exhibited less mitochondrial mass, lower ATP levels, and significantly lower basal oxygen consumption rate (OCR) and reserve capacity compared to normal skin keratinocytes. In contrast, in hypoxic conditions, cholesteatoma keratinocytes showed markedly higher levels in maximal OCR and reserve capacity, as well as lower proton leak OCRs, compared to normal skin keratinocytes. Hypoxia induced the reverse mitochondrial bioenergy profile from that in normoxia between these two types of keratinocytes, implying that an adaptive change of mitochondrial respiration to oxygen fluctuations may develop in cases of cholesteatoma. Such adaptation in response to hypoxic conditions may play a role in explaining the pathogenesis of acquired cholesteatoma. Nature Publishing Group UK 2018-01-09 /pmc/articles/PMC5760563/ /pubmed/29317713 http://dx.doi.org/10.1038/s41598-017-18536-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Shih, Cheng-Ping
Lee, Jen-Tin
Chen, Hang-Kang
Lin, Yi-Chun
Chen, Hsin-Chien
Lin, Yuan-Yung
Kuo, Chao-Yin
Chen, Yu-Ting
Wang, Chih-Hung
Comparison of changes in mitochondrial bioenergetics between keratinocytes in human external auditory canal skin and cholesteatomas from normoxia to hypoxia
title Comparison of changes in mitochondrial bioenergetics between keratinocytes in human external auditory canal skin and cholesteatomas from normoxia to hypoxia
title_full Comparison of changes in mitochondrial bioenergetics between keratinocytes in human external auditory canal skin and cholesteatomas from normoxia to hypoxia
title_fullStr Comparison of changes in mitochondrial bioenergetics between keratinocytes in human external auditory canal skin and cholesteatomas from normoxia to hypoxia
title_full_unstemmed Comparison of changes in mitochondrial bioenergetics between keratinocytes in human external auditory canal skin and cholesteatomas from normoxia to hypoxia
title_short Comparison of changes in mitochondrial bioenergetics between keratinocytes in human external auditory canal skin and cholesteatomas from normoxia to hypoxia
title_sort comparison of changes in mitochondrial bioenergetics between keratinocytes in human external auditory canal skin and cholesteatomas from normoxia to hypoxia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760563/
https://www.ncbi.nlm.nih.gov/pubmed/29317713
http://dx.doi.org/10.1038/s41598-017-18536-y
work_keys_str_mv AT shihchengping comparisonofchangesinmitochondrialbioenergeticsbetweenkeratinocytesinhumanexternalauditorycanalskinandcholesteatomasfromnormoxiatohypoxia
AT leejentin comparisonofchangesinmitochondrialbioenergeticsbetweenkeratinocytesinhumanexternalauditorycanalskinandcholesteatomasfromnormoxiatohypoxia
AT chenhangkang comparisonofchangesinmitochondrialbioenergeticsbetweenkeratinocytesinhumanexternalauditorycanalskinandcholesteatomasfromnormoxiatohypoxia
AT linyichun comparisonofchangesinmitochondrialbioenergeticsbetweenkeratinocytesinhumanexternalauditorycanalskinandcholesteatomasfromnormoxiatohypoxia
AT chenhsinchien comparisonofchangesinmitochondrialbioenergeticsbetweenkeratinocytesinhumanexternalauditorycanalskinandcholesteatomasfromnormoxiatohypoxia
AT linyuanyung comparisonofchangesinmitochondrialbioenergeticsbetweenkeratinocytesinhumanexternalauditorycanalskinandcholesteatomasfromnormoxiatohypoxia
AT kuochaoyin comparisonofchangesinmitochondrialbioenergeticsbetweenkeratinocytesinhumanexternalauditorycanalskinandcholesteatomasfromnormoxiatohypoxia
AT chenyuting comparisonofchangesinmitochondrialbioenergeticsbetweenkeratinocytesinhumanexternalauditorycanalskinandcholesteatomasfromnormoxiatohypoxia
AT wangchihhung comparisonofchangesinmitochondrialbioenergeticsbetweenkeratinocytesinhumanexternalauditorycanalskinandcholesteatomasfromnormoxiatohypoxia