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Methylation in Mycobacterium tuberculosis is lineage specific with associated mutations present globally

DNA methylation is an epigenetic modification of the genome involved in regulating crucial cellular processes, including transcription and chromosome stability. Advances in PacBio sequencing technologies can be used to robustly reveal methylation sites. The methylome of the Mycobacterium tuberculosi...

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Autores principales: Phelan, Jody, de Sessions, Paola Florez, Tientcheu, Leopold, Perdigao, Joao, Machado, Diana, Hasan, Rumina, Hasan, Zahra, Bergval, Indra L., Anthony, Richard, McNerney, Ruth, Antonio, Martin, Portugal, Isabel, Viveiros, Miguel, Campino, Susana, Hibberd, Martin L., Clark, Taane G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760664/
https://www.ncbi.nlm.nih.gov/pubmed/29317751
http://dx.doi.org/10.1038/s41598-017-18188-y
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author Phelan, Jody
de Sessions, Paola Florez
Tientcheu, Leopold
Perdigao, Joao
Machado, Diana
Hasan, Rumina
Hasan, Zahra
Bergval, Indra L.
Anthony, Richard
McNerney, Ruth
Antonio, Martin
Portugal, Isabel
Viveiros, Miguel
Campino, Susana
Hibberd, Martin L.
Clark, Taane G.
author_facet Phelan, Jody
de Sessions, Paola Florez
Tientcheu, Leopold
Perdigao, Joao
Machado, Diana
Hasan, Rumina
Hasan, Zahra
Bergval, Indra L.
Anthony, Richard
McNerney, Ruth
Antonio, Martin
Portugal, Isabel
Viveiros, Miguel
Campino, Susana
Hibberd, Martin L.
Clark, Taane G.
author_sort Phelan, Jody
collection PubMed
description DNA methylation is an epigenetic modification of the genome involved in regulating crucial cellular processes, including transcription and chromosome stability. Advances in PacBio sequencing technologies can be used to robustly reveal methylation sites. The methylome of the Mycobacterium tuberculosis complex is poorly understood but may be involved in virulence, hypoxic survival and the emergence of drug resistance. In the most extensive study to date, we characterise the methylome across the 4 major lineages of M. tuberculosis and 2 lineages of M. africanum, the leading causes of tuberculosis disease in humans. We reveal lineage-specific methylated motifs and strain-specific mutations that are abundant globally and likely to explain loss of function in the respective methyltransferases. Our work provides a set of sixteen new complete reference genomes for the Mycobacterium tuberculosis complex, including complete lineage 5 genomes. Insights into lineage-specific methylomes will further elucidate underlying biological mechanisms and other important phenotypes of the epi-genome.
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spelling pubmed-57606642018-01-17 Methylation in Mycobacterium tuberculosis is lineage specific with associated mutations present globally Phelan, Jody de Sessions, Paola Florez Tientcheu, Leopold Perdigao, Joao Machado, Diana Hasan, Rumina Hasan, Zahra Bergval, Indra L. Anthony, Richard McNerney, Ruth Antonio, Martin Portugal, Isabel Viveiros, Miguel Campino, Susana Hibberd, Martin L. Clark, Taane G. Sci Rep Article DNA methylation is an epigenetic modification of the genome involved in regulating crucial cellular processes, including transcription and chromosome stability. Advances in PacBio sequencing technologies can be used to robustly reveal methylation sites. The methylome of the Mycobacterium tuberculosis complex is poorly understood but may be involved in virulence, hypoxic survival and the emergence of drug resistance. In the most extensive study to date, we characterise the methylome across the 4 major lineages of M. tuberculosis and 2 lineages of M. africanum, the leading causes of tuberculosis disease in humans. We reveal lineage-specific methylated motifs and strain-specific mutations that are abundant globally and likely to explain loss of function in the respective methyltransferases. Our work provides a set of sixteen new complete reference genomes for the Mycobacterium tuberculosis complex, including complete lineage 5 genomes. Insights into lineage-specific methylomes will further elucidate underlying biological mechanisms and other important phenotypes of the epi-genome. Nature Publishing Group UK 2018-01-09 /pmc/articles/PMC5760664/ /pubmed/29317751 http://dx.doi.org/10.1038/s41598-017-18188-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Phelan, Jody
de Sessions, Paola Florez
Tientcheu, Leopold
Perdigao, Joao
Machado, Diana
Hasan, Rumina
Hasan, Zahra
Bergval, Indra L.
Anthony, Richard
McNerney, Ruth
Antonio, Martin
Portugal, Isabel
Viveiros, Miguel
Campino, Susana
Hibberd, Martin L.
Clark, Taane G.
Methylation in Mycobacterium tuberculosis is lineage specific with associated mutations present globally
title Methylation in Mycobacterium tuberculosis is lineage specific with associated mutations present globally
title_full Methylation in Mycobacterium tuberculosis is lineage specific with associated mutations present globally
title_fullStr Methylation in Mycobacterium tuberculosis is lineage specific with associated mutations present globally
title_full_unstemmed Methylation in Mycobacterium tuberculosis is lineage specific with associated mutations present globally
title_short Methylation in Mycobacterium tuberculosis is lineage specific with associated mutations present globally
title_sort methylation in mycobacterium tuberculosis is lineage specific with associated mutations present globally
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760664/
https://www.ncbi.nlm.nih.gov/pubmed/29317751
http://dx.doi.org/10.1038/s41598-017-18188-y
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