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Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria

In this report, spherical silver nanoparticle (AgNP-sp) and rod-shaped silver nanoparticle (AgNR) were prepared by chemical reduction method and their antibacterial activity against various Gram-positive and Gram-negative bacteria had been evaluated for their efficiency. Minimal inhibitory concentra...

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Autores principales: Acharya, Debashish, Singha, K. Malabika, Pandey, Piyush, Mohanta, Bidhan, Rajkumari, Jina, Singha, L. Paikhomba
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760701/
https://www.ncbi.nlm.nih.gov/pubmed/29317760
http://dx.doi.org/10.1038/s41598-017-18590-6
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author Acharya, Debashish
Singha, K. Malabika
Pandey, Piyush
Mohanta, Bidhan
Rajkumari, Jina
Singha, L. Paikhomba
author_facet Acharya, Debashish
Singha, K. Malabika
Pandey, Piyush
Mohanta, Bidhan
Rajkumari, Jina
Singha, L. Paikhomba
author_sort Acharya, Debashish
collection PubMed
description In this report, spherical silver nanoparticle (AgNP-sp) and rod-shaped silver nanoparticle (AgNR) were prepared by chemical reduction method and their antibacterial activity against various Gram-positive and Gram-negative bacteria had been evaluated for their efficiency. Minimal inhibitory concentration (MIC) tests were conducted to study the antibacterial properties, and substantiated with killing kinetics of silver nanoparticles (AgNPs). The study revealed that both AgNP-sp and AgNRs are good antibacterial candidates. Bacterial sensitivity to nanoparticles (NPs) was found to vary depending on microbial species. Disc diffusion studies revealed the greater effectiveness of AgNP-sp and AgNR against Klebsiella pneumoniae AWD5 at the doses of 249 and 392 µg. The dose dependent activities of prepared NPs were also observed on the batch studies of disc diffusion and MIC with various strains. The optical and morphological structures of NPs were analyzed by UV-visible, XRD, FE-SEM and TEM. Further, FESEM of bacterial culture treated with AgNPs confirmed antibacterial activity of NPs by showing rupture of bacterial cell wall. Also, the genome of test organism was found to have CusCFBA and CusRS operons. The killing kinetics confirmed that the death rate of K. pneumoniae was higher against AgNP-sp as compared to AgNR.
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spelling pubmed-57607012018-01-17 Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria Acharya, Debashish Singha, K. Malabika Pandey, Piyush Mohanta, Bidhan Rajkumari, Jina Singha, L. Paikhomba Sci Rep Article In this report, spherical silver nanoparticle (AgNP-sp) and rod-shaped silver nanoparticle (AgNR) were prepared by chemical reduction method and their antibacterial activity against various Gram-positive and Gram-negative bacteria had been evaluated for their efficiency. Minimal inhibitory concentration (MIC) tests were conducted to study the antibacterial properties, and substantiated with killing kinetics of silver nanoparticles (AgNPs). The study revealed that both AgNP-sp and AgNRs are good antibacterial candidates. Bacterial sensitivity to nanoparticles (NPs) was found to vary depending on microbial species. Disc diffusion studies revealed the greater effectiveness of AgNP-sp and AgNR against Klebsiella pneumoniae AWD5 at the doses of 249 and 392 µg. The dose dependent activities of prepared NPs were also observed on the batch studies of disc diffusion and MIC with various strains. The optical and morphological structures of NPs were analyzed by UV-visible, XRD, FE-SEM and TEM. Further, FESEM of bacterial culture treated with AgNPs confirmed antibacterial activity of NPs by showing rupture of bacterial cell wall. Also, the genome of test organism was found to have CusCFBA and CusRS operons. The killing kinetics confirmed that the death rate of K. pneumoniae was higher against AgNP-sp as compared to AgNR. Nature Publishing Group UK 2018-01-09 /pmc/articles/PMC5760701/ /pubmed/29317760 http://dx.doi.org/10.1038/s41598-017-18590-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Acharya, Debashish
Singha, K. Malabika
Pandey, Piyush
Mohanta, Bidhan
Rajkumari, Jina
Singha, L. Paikhomba
Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria
title Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria
title_full Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria
title_fullStr Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria
title_full_unstemmed Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria
title_short Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria
title_sort shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760701/
https://www.ncbi.nlm.nih.gov/pubmed/29317760
http://dx.doi.org/10.1038/s41598-017-18590-6
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