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Chemiluminescence determination of melamine with Luminol-K(3)Fe(CN)(6) system

A sensitive chemiluminescence (CL) method was developed for determining melamine in urine and plasma samples based on the fact that melamine can remarkably enhance the chemiluminescence of Luminol-K(3) Fe(CN)(6) system in alkaline medium. The determination conditions were optimized. Under optimum co...

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Detalles Bibliográficos
Autores principales: Tang, Xiao-Shuang, Shi, Xi-Yan, Tang, Yu-Hai, Yue, Zhong-Jin, He, Qi-Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Xi'an Jiaotong University 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760759/
https://www.ncbi.nlm.nih.gov/pubmed/29403687
http://dx.doi.org/10.1016/S2095-1779(11)70018-1
Descripción
Sumario:A sensitive chemiluminescence (CL) method was developed for determining melamine in urine and plasma samples based on the fact that melamine can remarkably enhance the chemiluminescence of Luminol-K(3) Fe(CN)(6) system in alkaline medium. The determination conditions were optimized. Under optimum conditions, the chemiluminescence intensity had a good linear relationship with melamine in the range of 9.0 × 10(−9) – 7.0 × 10(−6) g/mL with a correlation coefficient of 0.9992. The detection limits (3σ) were 3.54 ng/mL for urine sample and 6.58 ng/mL for plasma sample. The average recoveries of melamine were 102.6% for urine sample and 95.1% for plasma sample. Melamine in samples was extracted with liquid-liquid extraction procedures and the assay results coincided very well with that determined with flow injection chemiluminescence method. The method provides a reproducible and stable approach for sensitive detection and quantification of melamine in urine and plasma samples.