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Simultaneous determination of telmisartan and amlodipine in human plasma by LC–MS/MS and its application in a human pharmacokinetic study
A rapid and sensitive liquid chromatography–tandem mass spectrometric (LC–MS/MS) assay method has been developed and fully validated for the simultaneous quantification of telmisartan and amlodipine in human plasma. Carbamazepine was used as an internal standard. Analytes and the internal standard w...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Xi'an Jiaotong University
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760765/ https://www.ncbi.nlm.nih.gov/pubmed/29403761 http://dx.doi.org/10.1016/j.jpha.2012.03.008 |
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author | Ravi, Vasu Babu Inamadugu, Jaswanth Kumar Pilli, Nageswara Rao Sreenivasulu, Vudagandla Ponneri, Venkateswarlu |
author_facet | Ravi, Vasu Babu Inamadugu, Jaswanth Kumar Pilli, Nageswara Rao Sreenivasulu, Vudagandla Ponneri, Venkateswarlu |
author_sort | Ravi, Vasu Babu |
collection | PubMed |
description | A rapid and sensitive liquid chromatography–tandem mass spectrometric (LC–MS/MS) assay method has been developed and fully validated for the simultaneous quantification of telmisartan and amlodipine in human plasma. Carbamazepine was used as an internal standard. Analytes and the internal standard were extracted from human plasma by solid-phase extraction technique using Waters Oasis(®) HLB 1 cm(3) (30 mg) extraction cartridge. The reconstituted samples were chromatographed on a Hypurity advance C(18) column (50 mm×4.6 mm, 5 μm) using a mixture of acetonitrile–5 mM ammonium acetate buffer (pH-4.0) (50:50, v/v) as the mobile phase at a flow rate of 0.8 mL/min. The calibration curve obtained was linear (r≥0.99) over the concentration range of 2.01–400.06 ng/mL for telmisartan and 0.05–10.01 ng/mL for amlodipine. Method validation was performed as per FDA guidelines and the results met the acceptance criteria. A run time of 2.5 min for each sample made it possible to analyze more than 400 human plasma samples per day. The proposed method was found to be applicable to clinical studies. |
format | Online Article Text |
id | pubmed-5760765 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Xi'an Jiaotong University |
record_format | MEDLINE/PubMed |
spelling | pubmed-57607652018-02-05 Simultaneous determination of telmisartan and amlodipine in human plasma by LC–MS/MS and its application in a human pharmacokinetic study Ravi, Vasu Babu Inamadugu, Jaswanth Kumar Pilli, Nageswara Rao Sreenivasulu, Vudagandla Ponneri, Venkateswarlu J Pharm Anal Article A rapid and sensitive liquid chromatography–tandem mass spectrometric (LC–MS/MS) assay method has been developed and fully validated for the simultaneous quantification of telmisartan and amlodipine in human plasma. Carbamazepine was used as an internal standard. Analytes and the internal standard were extracted from human plasma by solid-phase extraction technique using Waters Oasis(®) HLB 1 cm(3) (30 mg) extraction cartridge. The reconstituted samples were chromatographed on a Hypurity advance C(18) column (50 mm×4.6 mm, 5 μm) using a mixture of acetonitrile–5 mM ammonium acetate buffer (pH-4.0) (50:50, v/v) as the mobile phase at a flow rate of 0.8 mL/min. The calibration curve obtained was linear (r≥0.99) over the concentration range of 2.01–400.06 ng/mL for telmisartan and 0.05–10.01 ng/mL for amlodipine. Method validation was performed as per FDA guidelines and the results met the acceptance criteria. A run time of 2.5 min for each sample made it possible to analyze more than 400 human plasma samples per day. The proposed method was found to be applicable to clinical studies. Xi'an Jiaotong University 2012-10 2012-04-04 /pmc/articles/PMC5760765/ /pubmed/29403761 http://dx.doi.org/10.1016/j.jpha.2012.03.008 Text en © 2012 Xi'an Jiaotong University http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
spellingShingle | Article Ravi, Vasu Babu Inamadugu, Jaswanth Kumar Pilli, Nageswara Rao Sreenivasulu, Vudagandla Ponneri, Venkateswarlu Simultaneous determination of telmisartan and amlodipine in human plasma by LC–MS/MS and its application in a human pharmacokinetic study |
title | Simultaneous determination of telmisartan and amlodipine in human plasma by LC–MS/MS and its application in a human pharmacokinetic study |
title_full | Simultaneous determination of telmisartan and amlodipine in human plasma by LC–MS/MS and its application in a human pharmacokinetic study |
title_fullStr | Simultaneous determination of telmisartan and amlodipine in human plasma by LC–MS/MS and its application in a human pharmacokinetic study |
title_full_unstemmed | Simultaneous determination of telmisartan and amlodipine in human plasma by LC–MS/MS and its application in a human pharmacokinetic study |
title_short | Simultaneous determination of telmisartan and amlodipine in human plasma by LC–MS/MS and its application in a human pharmacokinetic study |
title_sort | simultaneous determination of telmisartan and amlodipine in human plasma by lc–ms/ms and its application in a human pharmacokinetic study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760765/ https://www.ncbi.nlm.nih.gov/pubmed/29403761 http://dx.doi.org/10.1016/j.jpha.2012.03.008 |
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