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Simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture by high performance liquid chromatography

A reversed phase high performance liquid chromatography (HPLC) method was established for the simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture. A Grace Apollo C(18) column (250 mm×4.6 mm, 5 μm) was used as the stationary phase and the mobile phase was...

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Autores principales: Xie, Zhi-Sheng, Xu, Xin-Jun, Xie, Chun-Yan, Huang, Jie-Yun, Yang, Mei, Li, Rui-Ming, Chen, Xiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Xi'an Jiaotong University 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760806/
https://www.ncbi.nlm.nih.gov/pubmed/29403703
http://dx.doi.org/10.1016/j.jpha.2011.06.006
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author Xie, Zhi-Sheng
Xu, Xin-Jun
Xie, Chun-Yan
Huang, Jie-Yun
Yang, Mei
Li, Rui-Ming
Chen, Xiao
author_facet Xie, Zhi-Sheng
Xu, Xin-Jun
Xie, Chun-Yan
Huang, Jie-Yun
Yang, Mei
Li, Rui-Ming
Chen, Xiao
author_sort Xie, Zhi-Sheng
collection PubMed
description A reversed phase high performance liquid chromatography (HPLC) method was established for the simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture. A Grace Apollo C(18) column (250 mm×4.6 mm, 5 μm) was used as the stationary phase and the mobile phase was composed of acetonitrile and aqueous phosphoric acid (0.2%, v/v). Gradient elution was carried out at the flow rate of 1.0 mL/min and the column temperature was 30 °C. An ultraviolet (UV) detector was used with a selected wavelength of 240 nm. Calibration curves were linear within the concentration range of 4.6–45.75 μg/mL for 12, 13-dihydroxyeuparin (r>0.9999) and 106.9–1068.9 μg/mL for glycyrrhizic acid (r>0.9999), respectively. Recoveries were 102.18% for 12, 13-dihydroxyeuparin and 101.17% for glycyrrhizic acid. The method developed could be applied to the simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture.
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spelling pubmed-57608062018-02-05 Simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture by high performance liquid chromatography Xie, Zhi-Sheng Xu, Xin-Jun Xie, Chun-Yan Huang, Jie-Yun Yang, Mei Li, Rui-Ming Chen, Xiao J Pharm Anal Article A reversed phase high performance liquid chromatography (HPLC) method was established for the simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture. A Grace Apollo C(18) column (250 mm×4.6 mm, 5 μm) was used as the stationary phase and the mobile phase was composed of acetonitrile and aqueous phosphoric acid (0.2%, v/v). Gradient elution was carried out at the flow rate of 1.0 mL/min and the column temperature was 30 °C. An ultraviolet (UV) detector was used with a selected wavelength of 240 nm. Calibration curves were linear within the concentration range of 4.6–45.75 μg/mL for 12, 13-dihydroxyeuparin (r>0.9999) and 106.9–1068.9 μg/mL for glycyrrhizic acid (r>0.9999), respectively. Recoveries were 102.18% for 12, 13-dihydroxyeuparin and 101.17% for glycyrrhizic acid. The method developed could be applied to the simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture. Xi'an Jiaotong University 2011-08 2011-07-22 /pmc/articles/PMC5760806/ /pubmed/29403703 http://dx.doi.org/10.1016/j.jpha.2011.06.006 Text en © 2011 Xi'an Jiaotong University http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Article
Xie, Zhi-Sheng
Xu, Xin-Jun
Xie, Chun-Yan
Huang, Jie-Yun
Yang, Mei
Li, Rui-Ming
Chen, Xiao
Simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture by high performance liquid chromatography
title Simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture by high performance liquid chromatography
title_full Simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture by high performance liquid chromatography
title_fullStr Simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture by high performance liquid chromatography
title_full_unstemmed Simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture by high performance liquid chromatography
title_short Simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture by high performance liquid chromatography
title_sort simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in yanyanfang mixture by high performance liquid chromatography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760806/
https://www.ncbi.nlm.nih.gov/pubmed/29403703
http://dx.doi.org/10.1016/j.jpha.2011.06.006
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