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Voltammetric quantification of anti-hepatitis drug Adefovir in biological matrix and pharmaceutical formulation
Electrochemical reduction behavior of Adefovir was studied using Hanging Mercury Drop Electrode (HMDE) in Britton–Robinson (BR) buffer solution. Voltammetric study showed one well-defined reduction peak in the potential range −1.2 to −1.4 V (vs. Ag/AgCl) due to reduction of C=N bond of the imidazole...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Xi'an Jiaotong University
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760835/ https://www.ncbi.nlm.nih.gov/pubmed/29403728 http://dx.doi.org/10.1016/j.jpha.2011.10.002 |
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author | Jain, Rajeev Sharma, Ramkishor |
author_facet | Jain, Rajeev Sharma, Ramkishor |
author_sort | Jain, Rajeev |
collection | PubMed |
description | Electrochemical reduction behavior of Adefovir was studied using Hanging Mercury Drop Electrode (HMDE) in Britton–Robinson (BR) buffer solution. Voltammetric study showed one well-defined reduction peak in the potential range −1.2 to −1.4 V (vs. Ag/AgCl) due to reduction of C=N bond of the imidazole ring. Solid-phase extraction and protein-precipitation techniques were employed for extraction of Adefovir from human plasma. The proposed method allows quantification of Adefovir in human plasma over the concentration range 0.50–5.00 μg/mL with the detection limit 0.17 μg/mL, whereas in pharmaceutical formulation 0.25–2.25 μg/mL with the detection limit 0.08 μg/mL. |
format | Online Article Text |
id | pubmed-5760835 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Xi'an Jiaotong University |
record_format | MEDLINE/PubMed |
spelling | pubmed-57608352018-02-05 Voltammetric quantification of anti-hepatitis drug Adefovir in biological matrix and pharmaceutical formulation Jain, Rajeev Sharma, Ramkishor J Pharm Anal Article Electrochemical reduction behavior of Adefovir was studied using Hanging Mercury Drop Electrode (HMDE) in Britton–Robinson (BR) buffer solution. Voltammetric study showed one well-defined reduction peak in the potential range −1.2 to −1.4 V (vs. Ag/AgCl) due to reduction of C=N bond of the imidazole ring. Solid-phase extraction and protein-precipitation techniques were employed for extraction of Adefovir from human plasma. The proposed method allows quantification of Adefovir in human plasma over the concentration range 0.50–5.00 μg/mL with the detection limit 0.17 μg/mL, whereas in pharmaceutical formulation 0.25–2.25 μg/mL with the detection limit 0.08 μg/mL. Xi'an Jiaotong University 2012-04 2011-11-20 /pmc/articles/PMC5760835/ /pubmed/29403728 http://dx.doi.org/10.1016/j.jpha.2011.10.002 Text en © 2011 Xi'an Jiaotong University http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
spellingShingle | Article Jain, Rajeev Sharma, Ramkishor Voltammetric quantification of anti-hepatitis drug Adefovir in biological matrix and pharmaceutical formulation |
title | Voltammetric quantification of anti-hepatitis drug Adefovir in biological matrix and pharmaceutical formulation |
title_full | Voltammetric quantification of anti-hepatitis drug Adefovir in biological matrix and pharmaceutical formulation |
title_fullStr | Voltammetric quantification of anti-hepatitis drug Adefovir in biological matrix and pharmaceutical formulation |
title_full_unstemmed | Voltammetric quantification of anti-hepatitis drug Adefovir in biological matrix and pharmaceutical formulation |
title_short | Voltammetric quantification of anti-hepatitis drug Adefovir in biological matrix and pharmaceutical formulation |
title_sort | voltammetric quantification of anti-hepatitis drug adefovir in biological matrix and pharmaceutical formulation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760835/ https://www.ncbi.nlm.nih.gov/pubmed/29403728 http://dx.doi.org/10.1016/j.jpha.2011.10.002 |
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