Chiral separation of bavachinin in Fructus Psoraleae and rat plasma by liquid chromatography using permethylated-β-CD as a chiral selector

A simple, sensitive and selective method of high-performance liquid chromatography (HPLC) has been successfully developed for separation of bavachinin enantiomers in Fructus Psoraleae and rat plasma. The separation and detection conditions of HPLC were optimized. Chiral bavachinin were separated wit...

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Detalles Bibliográficos
Autores principales: Liu, Jing-Jing, Zhang, Juan, Chen, Zi-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Xi'an Jiaotong University 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760960/
https://www.ncbi.nlm.nih.gov/pubmed/29403838
http://dx.doi.org/10.1016/j.jpha.2012.12.010
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author Liu, Jing-Jing
Zhang, Juan
Chen, Zi-Lin
author_facet Liu, Jing-Jing
Zhang, Juan
Chen, Zi-Lin
author_sort Liu, Jing-Jing
collection PubMed
description A simple, sensitive and selective method of high-performance liquid chromatography (HPLC) has been successfully developed for separation of bavachinin enantiomers in Fructus Psoraleae and rat plasma. The separation and detection conditions of HPLC were optimized. Chiral bavachinin were separated with the mobile phase of methanol and water (70:30, v/v) at a flow rate of 1.0 mL/min. The linear ranges were in the range of 20–1000 μg/mL. The detection limits were tested as 4 ng/mL and 6 ng/mL for (+)-bavachinin and (−)-bavachinin, respectively. The method has been applied to analyze chiral bavachinin in rat plasma. HPLC–MS method was used to test the accuracy.
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spelling pubmed-57609602018-02-05 Chiral separation of bavachinin in Fructus Psoraleae and rat plasma by liquid chromatography using permethylated-β-CD as a chiral selector Liu, Jing-Jing Zhang, Juan Chen, Zi-Lin J Pharm Anal Research Article A simple, sensitive and selective method of high-performance liquid chromatography (HPLC) has been successfully developed for separation of bavachinin enantiomers in Fructus Psoraleae and rat plasma. The separation and detection conditions of HPLC were optimized. Chiral bavachinin were separated with the mobile phase of methanol and water (70:30, v/v) at a flow rate of 1.0 mL/min. The linear ranges were in the range of 20–1000 μg/mL. The detection limits were tested as 4 ng/mL and 6 ng/mL for (+)-bavachinin and (−)-bavachinin, respectively. The method has been applied to analyze chiral bavachinin in rat plasma. HPLC–MS method was used to test the accuracy. Xi'an Jiaotong University 2013-10 2013-01-23 /pmc/articles/PMC5760960/ /pubmed/29403838 http://dx.doi.org/10.1016/j.jpha.2012.12.010 Text en © 2013 Xi’an Jiaotong University. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Research Article
Liu, Jing-Jing
Zhang, Juan
Chen, Zi-Lin
Chiral separation of bavachinin in Fructus Psoraleae and rat plasma by liquid chromatography using permethylated-β-CD as a chiral selector
title Chiral separation of bavachinin in Fructus Psoraleae and rat plasma by liquid chromatography using permethylated-β-CD as a chiral selector
title_full Chiral separation of bavachinin in Fructus Psoraleae and rat plasma by liquid chromatography using permethylated-β-CD as a chiral selector
title_fullStr Chiral separation of bavachinin in Fructus Psoraleae and rat plasma by liquid chromatography using permethylated-β-CD as a chiral selector
title_full_unstemmed Chiral separation of bavachinin in Fructus Psoraleae and rat plasma by liquid chromatography using permethylated-β-CD as a chiral selector
title_short Chiral separation of bavachinin in Fructus Psoraleae and rat plasma by liquid chromatography using permethylated-β-CD as a chiral selector
title_sort chiral separation of bavachinin in fructus psoraleae and rat plasma by liquid chromatography using permethylated-β-cd as a chiral selector
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760960/
https://www.ncbi.nlm.nih.gov/pubmed/29403838
http://dx.doi.org/10.1016/j.jpha.2012.12.010
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