Simultaneous determination of five diterpenoid alkaloids in Herba Delphinii by HPLC/ELSD

A HPLC–ELSD method was developed and validated for simultaneous determination of five Hetisane-type diterpenoid alkaloids in a Tibetan traditional herbal medicine, “Gebu Dilu” (Herba Delphinii), using a Kromasil C(18) column (250 mm×4.6 mm, 5 μm) with the mobile phase consisting of acetonitrile and...

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Detalles Bibliográficos
Autores principales: Lin, Chao-Zhan, Xie, Si-Min, Zhu, Chen-Chen, Bairu, Zeren-Dawa, Kangsa, Suolang-Qimei, Zhu, Dun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Xi'an Jiaotong University 2013
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761006/
https://www.ncbi.nlm.nih.gov/pubmed/29403853
http://dx.doi.org/10.1016/j.jpha.2013.06.002
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author Lin, Chao-Zhan
Xie, Si-Min
Zhu, Chen-Chen
Bairu, Zeren-Dawa
Kangsa, Suolang-Qimei
Zhu, Dun
author_facet Lin, Chao-Zhan
Xie, Si-Min
Zhu, Chen-Chen
Bairu, Zeren-Dawa
Kangsa, Suolang-Qimei
Zhu, Dun
author_sort Lin, Chao-Zhan
collection PubMed
description A HPLC–ELSD method was developed and validated for simultaneous determination of five Hetisane-type diterpenoid alkaloids in a Tibetan traditional herbal medicine, “Gebu Dilu” (Herba Delphinii), using a Kromasil C(18) column (250 mm×4.6 mm, 5 μm) with the mobile phase consisting of acetonitrile and 0.1% triethylamine in gradient (detected by evaporative light scattering detector). The linear ranges of five compounds were determined and method validation was evaluated completely. The established method is rapid and accurate with high repeatability, and can be applied for the quality control of Herba Delphinii.
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spelling pubmed-57610062018-02-05 Simultaneous determination of five diterpenoid alkaloids in Herba Delphinii by HPLC/ELSD Lin, Chao-Zhan Xie, Si-Min Zhu, Chen-Chen Bairu, Zeren-Dawa Kangsa, Suolang-Qimei Zhu, Dun J Pharm Anal Short Communication A HPLC–ELSD method was developed and validated for simultaneous determination of five Hetisane-type diterpenoid alkaloids in a Tibetan traditional herbal medicine, “Gebu Dilu” (Herba Delphinii), using a Kromasil C(18) column (250 mm×4.6 mm, 5 μm) with the mobile phase consisting of acetonitrile and 0.1% triethylamine in gradient (detected by evaporative light scattering detector). The linear ranges of five compounds were determined and method validation was evaluated completely. The established method is rapid and accurate with high repeatability, and can be applied for the quality control of Herba Delphinii. Xi'an Jiaotong University 2013-12 2013-07-06 /pmc/articles/PMC5761006/ /pubmed/29403853 http://dx.doi.org/10.1016/j.jpha.2013.06.002 Text en © 2013 Xi’an Jiaotong University. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Short Communication
Lin, Chao-Zhan
Xie, Si-Min
Zhu, Chen-Chen
Bairu, Zeren-Dawa
Kangsa, Suolang-Qimei
Zhu, Dun
Simultaneous determination of five diterpenoid alkaloids in Herba Delphinii by HPLC/ELSD
title Simultaneous determination of five diterpenoid alkaloids in Herba Delphinii by HPLC/ELSD
title_full Simultaneous determination of five diterpenoid alkaloids in Herba Delphinii by HPLC/ELSD
title_fullStr Simultaneous determination of five diterpenoid alkaloids in Herba Delphinii by HPLC/ELSD
title_full_unstemmed Simultaneous determination of five diterpenoid alkaloids in Herba Delphinii by HPLC/ELSD
title_short Simultaneous determination of five diterpenoid alkaloids in Herba Delphinii by HPLC/ELSD
title_sort simultaneous determination of five diterpenoid alkaloids in herba delphinii by hplc/elsd
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761006/
https://www.ncbi.nlm.nih.gov/pubmed/29403853
http://dx.doi.org/10.1016/j.jpha.2013.06.002
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