Preparative isolation of Heteroclitin D from Kadsurae Caulis using normal-phase flash chromatography

Heteroclitin D (H.D) was successfully isolated from Kadsurae Caulis by using flash chromatography and recrystallized by methanol, 10.2 mg of H.D was obtained from 4.86 g of crude extract, and the purity determined by HPLC was 99.4%. The structure was identified by UV, IR, MS, and NMR analysis. The f...

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Detalles Bibliográficos
Autores principales: Yu, Xiao-Xue, Wang, Qian-Wen, Xu, Xin-Jun, Lv, Wei-Jian, Zhao, Ming-Qian, Liang, Zhi-Kun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Xi'an Jiaotong University 2013
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761017/
https://www.ncbi.nlm.nih.gov/pubmed/29403855
http://dx.doi.org/10.1016/j.jpha.2013.07.004
Descripción
Sumario:Heteroclitin D (H.D) was successfully isolated from Kadsurae Caulis by using flash chromatography and recrystallized by methanol, 10.2 mg of H.D was obtained from 4.86 g of crude extract, and the purity determined by HPLC was 99.4%. The structure was identified by UV, IR, MS, and NMR analysis. The fast, simple and efficient method can be applied to the preparation of reference substance of H. D.

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