Preparative isolation of Heteroclitin D from Kadsurae Caulis using normal-phase flash chromatography

Heteroclitin D (H.D) was successfully isolated from Kadsurae Caulis by using flash chromatography and recrystallized by methanol, 10.2 mg of H.D was obtained from 4.86 g of crude extract, and the purity determined by HPLC was 99.4%. The structure was identified by UV, IR, MS, and NMR analysis. The f...

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Detalles Bibliográficos
Autores principales: Yu, Xiao-Xue, Wang, Qian-Wen, Xu, Xin-Jun, Lv, Wei-Jian, Zhao, Ming-Qian, Liang, Zhi-Kun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Xi'an Jiaotong University 2013
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761017/
https://www.ncbi.nlm.nih.gov/pubmed/29403855
http://dx.doi.org/10.1016/j.jpha.2013.07.004
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author Yu, Xiao-Xue
Wang, Qian-Wen
Xu, Xin-Jun
Lv, Wei-Jian
Zhao, Ming-Qian
Liang, Zhi-Kun
author_facet Yu, Xiao-Xue
Wang, Qian-Wen
Xu, Xin-Jun
Lv, Wei-Jian
Zhao, Ming-Qian
Liang, Zhi-Kun
author_sort Yu, Xiao-Xue
collection PubMed
description Heteroclitin D (H.D) was successfully isolated from Kadsurae Caulis by using flash chromatography and recrystallized by methanol, 10.2 mg of H.D was obtained from 4.86 g of crude extract, and the purity determined by HPLC was 99.4%. The structure was identified by UV, IR, MS, and NMR analysis. The fast, simple and efficient method can be applied to the preparation of reference substance of H. D.
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spelling pubmed-57610172018-02-05 Preparative isolation of Heteroclitin D from Kadsurae Caulis using normal-phase flash chromatography Yu, Xiao-Xue Wang, Qian-Wen Xu, Xin-Jun Lv, Wei-Jian Zhao, Ming-Qian Liang, Zhi-Kun J Pharm Anal Short Communication Heteroclitin D (H.D) was successfully isolated from Kadsurae Caulis by using flash chromatography and recrystallized by methanol, 10.2 mg of H.D was obtained from 4.86 g of crude extract, and the purity determined by HPLC was 99.4%. The structure was identified by UV, IR, MS, and NMR analysis. The fast, simple and efficient method can be applied to the preparation of reference substance of H. D. Xi'an Jiaotong University 2013-12 2013-08-07 /pmc/articles/PMC5761017/ /pubmed/29403855 http://dx.doi.org/10.1016/j.jpha.2013.07.004 Text en © 2013 Xi’an Jiaotong University. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Short Communication
Yu, Xiao-Xue
Wang, Qian-Wen
Xu, Xin-Jun
Lv, Wei-Jian
Zhao, Ming-Qian
Liang, Zhi-Kun
Preparative isolation of Heteroclitin D from Kadsurae Caulis using normal-phase flash chromatography
title Preparative isolation of Heteroclitin D from Kadsurae Caulis using normal-phase flash chromatography
title_full Preparative isolation of Heteroclitin D from Kadsurae Caulis using normal-phase flash chromatography
title_fullStr Preparative isolation of Heteroclitin D from Kadsurae Caulis using normal-phase flash chromatography
title_full_unstemmed Preparative isolation of Heteroclitin D from Kadsurae Caulis using normal-phase flash chromatography
title_short Preparative isolation of Heteroclitin D from Kadsurae Caulis using normal-phase flash chromatography
title_sort preparative isolation of heteroclitin d from kadsurae caulis using normal-phase flash chromatography
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761017/
https://www.ncbi.nlm.nih.gov/pubmed/29403855
http://dx.doi.org/10.1016/j.jpha.2013.07.004
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