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Development and validation of an rp-hplc method for simultaneous determination of Ramipril and Amlodipine in tablets

An rp-hplc method for the simultaneous determination of Ramipril (RP) and Amlodipine (AL) in tablets was developed and validated by Chinese Pharmacopoeia 2010. The linearity of the proposed method was investigated in the range of 0.01–0.25 mg/mL (r(2)=0.9998) for RP and 0.014–0.36 mg/mL (r(2)=0.9997...

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Detalles Bibliográficos
Autores principales: Dai, Shi-Ying, Qiu, Shi-Ting, Wu, Wei, Fu, Chun-Mei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Xi'an Jiaotong University 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761019/
https://www.ncbi.nlm.nih.gov/pubmed/29403852
http://dx.doi.org/10.1016/j.jpha.2013.09.002
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author Dai, Shi-Ying
Qiu, Shi-Ting
Wu, Wei
Fu, Chun-Mei
author_facet Dai, Shi-Ying
Qiu, Shi-Ting
Wu, Wei
Fu, Chun-Mei
author_sort Dai, Shi-Ying
collection PubMed
description An rp-hplc method for the simultaneous determination of Ramipril (RP) and Amlodipine (AL) in tablets was developed and validated by Chinese Pharmacopoeia 2010. The linearity of the proposed method was investigated in the range of 0.01–0.25 mg/mL (r(2)=0.9998) for RP and 0.014–0.36 mg/mL (r(2)=0.9997) for AL. The limits of detection (LOD) were 0.06 μg/mL and 0.02 μg/mL for RP and AL, and the limits of quantitation (LOQ) were 0.2 μg/mL and 0.07 μg/mL, respectively. Some major impurities and degradation products did not disturb the detection of RP and AL and the assay can thus be considered stability-indicating.
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spelling pubmed-57610192018-02-05 Development and validation of an rp-hplc method for simultaneous determination of Ramipril and Amlodipine in tablets Dai, Shi-Ying Qiu, Shi-Ting Wu, Wei Fu, Chun-Mei J Pharm Anal Original Article An rp-hplc method for the simultaneous determination of Ramipril (RP) and Amlodipine (AL) in tablets was developed and validated by Chinese Pharmacopoeia 2010. The linearity of the proposed method was investigated in the range of 0.01–0.25 mg/mL (r(2)=0.9998) for RP and 0.014–0.36 mg/mL (r(2)=0.9997) for AL. The limits of detection (LOD) were 0.06 μg/mL and 0.02 μg/mL for RP and AL, and the limits of quantitation (LOQ) were 0.2 μg/mL and 0.07 μg/mL, respectively. Some major impurities and degradation products did not disturb the detection of RP and AL and the assay can thus be considered stability-indicating. Xi'an Jiaotong University 2013-12 2013-10-02 /pmc/articles/PMC5761019/ /pubmed/29403852 http://dx.doi.org/10.1016/j.jpha.2013.09.002 Text en © 2013 Xi’an Jiaotong University. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Original Article
Dai, Shi-Ying
Qiu, Shi-Ting
Wu, Wei
Fu, Chun-Mei
Development and validation of an rp-hplc method for simultaneous determination of Ramipril and Amlodipine in tablets
title Development and validation of an rp-hplc method for simultaneous determination of Ramipril and Amlodipine in tablets
title_full Development and validation of an rp-hplc method for simultaneous determination of Ramipril and Amlodipine in tablets
title_fullStr Development and validation of an rp-hplc method for simultaneous determination of Ramipril and Amlodipine in tablets
title_full_unstemmed Development and validation of an rp-hplc method for simultaneous determination of Ramipril and Amlodipine in tablets
title_short Development and validation of an rp-hplc method for simultaneous determination of Ramipril and Amlodipine in tablets
title_sort development and validation of an rp-hplc method for simultaneous determination of ramipril and amlodipine in tablets
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761019/
https://www.ncbi.nlm.nih.gov/pubmed/29403852
http://dx.doi.org/10.1016/j.jpha.2013.09.002
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