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Development and validation of an rp-hplc method for simultaneous determination of Ramipril and Amlodipine in tablets
An rp-hplc method for the simultaneous determination of Ramipril (RP) and Amlodipine (AL) in tablets was developed and validated by Chinese Pharmacopoeia 2010. The linearity of the proposed method was investigated in the range of 0.01–0.25 mg/mL (r(2)=0.9998) for RP and 0.014–0.36 mg/mL (r(2)=0.9997...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Xi'an Jiaotong University
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761019/ https://www.ncbi.nlm.nih.gov/pubmed/29403852 http://dx.doi.org/10.1016/j.jpha.2013.09.002 |
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author | Dai, Shi-Ying Qiu, Shi-Ting Wu, Wei Fu, Chun-Mei |
author_facet | Dai, Shi-Ying Qiu, Shi-Ting Wu, Wei Fu, Chun-Mei |
author_sort | Dai, Shi-Ying |
collection | PubMed |
description | An rp-hplc method for the simultaneous determination of Ramipril (RP) and Amlodipine (AL) in tablets was developed and validated by Chinese Pharmacopoeia 2010. The linearity of the proposed method was investigated in the range of 0.01–0.25 mg/mL (r(2)=0.9998) for RP and 0.014–0.36 mg/mL (r(2)=0.9997) for AL. The limits of detection (LOD) were 0.06 μg/mL and 0.02 μg/mL for RP and AL, and the limits of quantitation (LOQ) were 0.2 μg/mL and 0.07 μg/mL, respectively. Some major impurities and degradation products did not disturb the detection of RP and AL and the assay can thus be considered stability-indicating. |
format | Online Article Text |
id | pubmed-5761019 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Xi'an Jiaotong University |
record_format | MEDLINE/PubMed |
spelling | pubmed-57610192018-02-05 Development and validation of an rp-hplc method for simultaneous determination of Ramipril and Amlodipine in tablets Dai, Shi-Ying Qiu, Shi-Ting Wu, Wei Fu, Chun-Mei J Pharm Anal Original Article An rp-hplc method for the simultaneous determination of Ramipril (RP) and Amlodipine (AL) in tablets was developed and validated by Chinese Pharmacopoeia 2010. The linearity of the proposed method was investigated in the range of 0.01–0.25 mg/mL (r(2)=0.9998) for RP and 0.014–0.36 mg/mL (r(2)=0.9997) for AL. The limits of detection (LOD) were 0.06 μg/mL and 0.02 μg/mL for RP and AL, and the limits of quantitation (LOQ) were 0.2 μg/mL and 0.07 μg/mL, respectively. Some major impurities and degradation products did not disturb the detection of RP and AL and the assay can thus be considered stability-indicating. Xi'an Jiaotong University 2013-12 2013-10-02 /pmc/articles/PMC5761019/ /pubmed/29403852 http://dx.doi.org/10.1016/j.jpha.2013.09.002 Text en © 2013 Xi’an Jiaotong University. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
spellingShingle | Original Article Dai, Shi-Ying Qiu, Shi-Ting Wu, Wei Fu, Chun-Mei Development and validation of an rp-hplc method for simultaneous determination of Ramipril and Amlodipine in tablets |
title | Development and validation of an rp-hplc method for simultaneous determination of Ramipril and Amlodipine in tablets |
title_full | Development and validation of an rp-hplc method for simultaneous determination of Ramipril and Amlodipine in tablets |
title_fullStr | Development and validation of an rp-hplc method for simultaneous determination of Ramipril and Amlodipine in tablets |
title_full_unstemmed | Development and validation of an rp-hplc method for simultaneous determination of Ramipril and Amlodipine in tablets |
title_short | Development and validation of an rp-hplc method for simultaneous determination of Ramipril and Amlodipine in tablets |
title_sort | development and validation of an rp-hplc method for simultaneous determination of ramipril and amlodipine in tablets |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761019/ https://www.ncbi.nlm.nih.gov/pubmed/29403852 http://dx.doi.org/10.1016/j.jpha.2013.09.002 |
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