Simultaneous determination of borneol and its metabolite in rat plasma by GC–MS and its application to pharmacokinetic study

A gas chromatography mass spectrometry (GC–MS) method has been developed and fully validated for the simultaneous determination of natural borneol (NB) and its metabolite, camphor, in rat plasma. Following a single liquid–liquid extraction, the analytes were separated using an HP-5MS capillary colum...

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Detalles Bibliográficos
Autores principales: Sun, Xiu-Man, Liao, Qiong-Feng, Zhou, Yu-Ting, Deng, Xue-Jiao, Xie, Zhi-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Xi'an Jiaotong University 2014
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761216/
https://www.ncbi.nlm.nih.gov/pubmed/29403899
http://dx.doi.org/10.1016/j.jpha.2014.01.005
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author Sun, Xiu-Man
Liao, Qiong-Feng
Zhou, Yu-Ting
Deng, Xue-Jiao
Xie, Zhi-Yong
author_facet Sun, Xiu-Man
Liao, Qiong-Feng
Zhou, Yu-Ting
Deng, Xue-Jiao
Xie, Zhi-Yong
author_sort Sun, Xiu-Man
collection PubMed
description A gas chromatography mass spectrometry (GC–MS) method has been developed and fully validated for the simultaneous determination of natural borneol (NB) and its metabolite, camphor, in rat plasma. Following a single liquid–liquid extraction, the analytes were separated using an HP-5MS capillary column (0.25 mm×30 m×0.25 μm) and analyzed by MS in the selected ion monitoring mode. Selected ion monitor (m/z) of borneol, camphor and internal standard was 95, 95 and 128, respectively. Linearity, accuracy, precision and extraction recovery of the analytes were all satisfactory. The method was successfully applied to pharmacokinetic studies of NB after oral administration to Wistar rats.
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spelling pubmed-57612162018-02-05 Simultaneous determination of borneol and its metabolite in rat plasma by GC–MS and its application to pharmacokinetic study Sun, Xiu-Man Liao, Qiong-Feng Zhou, Yu-Ting Deng, Xue-Jiao Xie, Zhi-Yong J Pharm Anal Short Communication A gas chromatography mass spectrometry (GC–MS) method has been developed and fully validated for the simultaneous determination of natural borneol (NB) and its metabolite, camphor, in rat plasma. Following a single liquid–liquid extraction, the analytes were separated using an HP-5MS capillary column (0.25 mm×30 m×0.25 μm) and analyzed by MS in the selected ion monitoring mode. Selected ion monitor (m/z) of borneol, camphor and internal standard was 95, 95 and 128, respectively. Linearity, accuracy, precision and extraction recovery of the analytes were all satisfactory. The method was successfully applied to pharmacokinetic studies of NB after oral administration to Wistar rats. Xi'an Jiaotong University 2014-10 2014-02-15 /pmc/articles/PMC5761216/ /pubmed/29403899 http://dx.doi.org/10.1016/j.jpha.2014.01.005 Text en © 2014 Xi’an Jiaotong University. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Short Communication
Sun, Xiu-Man
Liao, Qiong-Feng
Zhou, Yu-Ting
Deng, Xue-Jiao
Xie, Zhi-Yong
Simultaneous determination of borneol and its metabolite in rat plasma by GC–MS and its application to pharmacokinetic study
title Simultaneous determination of borneol and its metabolite in rat plasma by GC–MS and its application to pharmacokinetic study
title_full Simultaneous determination of borneol and its metabolite in rat plasma by GC–MS and its application to pharmacokinetic study
title_fullStr Simultaneous determination of borneol and its metabolite in rat plasma by GC–MS and its application to pharmacokinetic study
title_full_unstemmed Simultaneous determination of borneol and its metabolite in rat plasma by GC–MS and its application to pharmacokinetic study
title_short Simultaneous determination of borneol and its metabolite in rat plasma by GC–MS and its application to pharmacokinetic study
title_sort simultaneous determination of borneol and its metabolite in rat plasma by gc–ms and its application to pharmacokinetic study
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761216/
https://www.ncbi.nlm.nih.gov/pubmed/29403899
http://dx.doi.org/10.1016/j.jpha.2014.01.005
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