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Vibrio cholerae genomic diversity within and between patients
Cholera is a severe, water-borne diarrhoeal disease caused by toxin-producing strains of the bacterium Vibrio cholerae. Comparative genomics has revealed ‘waves’ of cholera transmission and evolution, in which clones are successively replaced over decades and centuries. However, the extent of V. cho...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Microbiology Society
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761273/ https://www.ncbi.nlm.nih.gov/pubmed/29306353 http://dx.doi.org/10.1099/mgen.0.000142 |
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author | Levade, Inès Terrat, Yves Leducq, Jean-Baptiste Weil, Ana A. Mayo-Smith, Leslie M. Chowdhury, Fahima Khan, Ashraful I. Boncy, Jacques Buteau, Josiane Ivers, Louise C. Ryan, Edward T. Charles, Richelle C. Calderwood, Stephen B. Qadri, Firdausi Harris, Jason B. LaRocque, Regina C. Shapiro, B. Jesse |
author_facet | Levade, Inès Terrat, Yves Leducq, Jean-Baptiste Weil, Ana A. Mayo-Smith, Leslie M. Chowdhury, Fahima Khan, Ashraful I. Boncy, Jacques Buteau, Josiane Ivers, Louise C. Ryan, Edward T. Charles, Richelle C. Calderwood, Stephen B. Qadri, Firdausi Harris, Jason B. LaRocque, Regina C. Shapiro, B. Jesse |
author_sort | Levade, Inès |
collection | PubMed |
description | Cholera is a severe, water-borne diarrhoeal disease caused by toxin-producing strains of the bacterium Vibrio cholerae. Comparative genomics has revealed ‘waves’ of cholera transmission and evolution, in which clones are successively replaced over decades and centuries. However, the extent of V. cholerae genetic diversity within an epidemic or even within an individual patient is poorly understood. Here, we characterized V. cholerae genomic diversity at a micro-epidemiological level within and between individual patients from Bangladesh and Haiti. To capture within-patient diversity, we isolated multiple (8 to 20) V. cholerae colonies from each of eight patients, sequenced their genomes and identified point mutations and gene gain/loss events. We found limited but detectable diversity at the level of point mutations within hosts (zero to three single nucleotide variants within each patient), and comparatively higher gene content variation within hosts (at least one gain/loss event per patient, and up to 103 events in one patient). Much of the gene content variation appeared to be due to gain and loss of phage and plasmids within the V. cholerae population, with occasional exchanges between V. cholerae and other members of the gut microbiota. We also show that certain intra-host variants have phenotypic consequences. For example, the acquisition of a Bacteroides plasmid and non-synonymous mutations in a sensor histidine kinase gene both reduced biofilm formation, an important trait for environmental survival. Together, our results show that V. cholerae is measurably evolving within patients, with possible implications for disease outcomes and transmission dynamics. |
format | Online Article Text |
id | pubmed-5761273 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Microbiology Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-57612732018-01-12 Vibrio cholerae genomic diversity within and between patients Levade, Inès Terrat, Yves Leducq, Jean-Baptiste Weil, Ana A. Mayo-Smith, Leslie M. Chowdhury, Fahima Khan, Ashraful I. Boncy, Jacques Buteau, Josiane Ivers, Louise C. Ryan, Edward T. Charles, Richelle C. Calderwood, Stephen B. Qadri, Firdausi Harris, Jason B. LaRocque, Regina C. Shapiro, B. Jesse Microb Genom Research Article Cholera is a severe, water-borne diarrhoeal disease caused by toxin-producing strains of the bacterium Vibrio cholerae. Comparative genomics has revealed ‘waves’ of cholera transmission and evolution, in which clones are successively replaced over decades and centuries. However, the extent of V. cholerae genetic diversity within an epidemic or even within an individual patient is poorly understood. Here, we characterized V. cholerae genomic diversity at a micro-epidemiological level within and between individual patients from Bangladesh and Haiti. To capture within-patient diversity, we isolated multiple (8 to 20) V. cholerae colonies from each of eight patients, sequenced their genomes and identified point mutations and gene gain/loss events. We found limited but detectable diversity at the level of point mutations within hosts (zero to three single nucleotide variants within each patient), and comparatively higher gene content variation within hosts (at least one gain/loss event per patient, and up to 103 events in one patient). Much of the gene content variation appeared to be due to gain and loss of phage and plasmids within the V. cholerae population, with occasional exchanges between V. cholerae and other members of the gut microbiota. We also show that certain intra-host variants have phenotypic consequences. For example, the acquisition of a Bacteroides plasmid and non-synonymous mutations in a sensor histidine kinase gene both reduced biofilm formation, an important trait for environmental survival. Together, our results show that V. cholerae is measurably evolving within patients, with possible implications for disease outcomes and transmission dynamics. Microbiology Society 2017-12-07 /pmc/articles/PMC5761273/ /pubmed/29306353 http://dx.doi.org/10.1099/mgen.0.000142 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Levade, Inès Terrat, Yves Leducq, Jean-Baptiste Weil, Ana A. Mayo-Smith, Leslie M. Chowdhury, Fahima Khan, Ashraful I. Boncy, Jacques Buteau, Josiane Ivers, Louise C. Ryan, Edward T. Charles, Richelle C. Calderwood, Stephen B. Qadri, Firdausi Harris, Jason B. LaRocque, Regina C. Shapiro, B. Jesse Vibrio cholerae genomic diversity within and between patients |
title | Vibrio cholerae genomic diversity within and between patients |
title_full | Vibrio cholerae genomic diversity within and between patients |
title_fullStr | Vibrio cholerae genomic diversity within and between patients |
title_full_unstemmed | Vibrio cholerae genomic diversity within and between patients |
title_short | Vibrio cholerae genomic diversity within and between patients |
title_sort | vibrio cholerae genomic diversity within and between patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761273/ https://www.ncbi.nlm.nih.gov/pubmed/29306353 http://dx.doi.org/10.1099/mgen.0.000142 |
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