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Motile Salmonella serotypes causing high mortality in poultry farms in three South-Western States of Nigeria

This study was carried out to identify the Salmonella serotypes causing high mortality in chickens in Lagos, Ogun and Oyo states, Nigeria. Chickens presented for postmortem examination during disease outbreaks that were characterised by high mortality (40 per cent to 80 per cent) in poultry farms in...

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Autores principales: Mshelbwala, Fakilahyel Musa, Ibrahim, Najume Doguwar-Giginya, Saidu, Shehu NaAllah, Azeez, Aminullah Ajiyobiojo, Akinduti, Paul Akinniyi, Kwanashie, Clara Nna, Fakilahyel Kadiri, Amina Kinta, Muhammed, Maryam, Fagbamila, Idowu Oluwabunmi, Luka, Pam Dachung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761298/
https://www.ncbi.nlm.nih.gov/pubmed/29344363
http://dx.doi.org/10.1136/vetreco-2017-000247
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author Mshelbwala, Fakilahyel Musa
Ibrahim, Najume Doguwar-Giginya
Saidu, Shehu NaAllah
Azeez, Aminullah Ajiyobiojo
Akinduti, Paul Akinniyi
Kwanashie, Clara Nna
Fakilahyel Kadiri, Amina Kinta
Muhammed, Maryam
Fagbamila, Idowu Oluwabunmi
Luka, Pam Dachung
author_facet Mshelbwala, Fakilahyel Musa
Ibrahim, Najume Doguwar-Giginya
Saidu, Shehu NaAllah
Azeez, Aminullah Ajiyobiojo
Akinduti, Paul Akinniyi
Kwanashie, Clara Nna
Fakilahyel Kadiri, Amina Kinta
Muhammed, Maryam
Fagbamila, Idowu Oluwabunmi
Luka, Pam Dachung
author_sort Mshelbwala, Fakilahyel Musa
collection PubMed
description This study was carried out to identify the Salmonella serotypes causing high mortality in chickens in Lagos, Ogun and Oyo states, Nigeria. Chickens presented for postmortem examination during disease outbreaks that were characterised by high mortality (40 per cent to 80 per cent) in poultry farms in the study area were examined from January to December, 2013. Samples of the lungs, heart, liver, spleen, kidneys, proventriculus, intestine and caecum were collected from suspected cases of salmonellosis, for bacterial culture and identification. Salmonella isolates were confirmed using PCR and serotyped using the Kauffman-White scheme. Twenty-six day-old pullets were raised to two weeks and inoculated orally with 0.2 mL of 1×10(8) colony forming units of Salmonella Zega identified in the present study to determine their pathogenicity, while another 26 served as control. The Salmonella serotypes were S Zega (n=13; 35.14 per cent), Salmonella Kentucky (n=9; 24.32 per cent), Salmonella Herston (n=6; 16.22 per cent), Salmonella Nima (n=4; 10.81 per cent), Salmonella Telelkebir (n=3; 8.11 per cent), Salmonella Colindale (n=1; 2.70 per cent) and Salmonella Tshiongwe (n=1; 2.70 per cent). Clinical signs in both natural and experimental infections were acute (70 per cent) and chronic (30 per cent), and included weakness, anorexia, yellowish diarrhoea, pasted vents, somnolescence and mortality, while gross lesions showed marked pulmonary congestion and oedema, necrotic foci in the myocardium; the liver, spleen and kidneys were markedly enlarged and had subcapsular multifocal necrosis. There were catarrhal proventriculitis and enteritis, and haemorrhagic typhlitis. While most of the serotypes identified in the present study have been isolated from poultry sources from commercial farms in Nigeria, to the best of the authors' knowledge, they have not been previously reported to cause high mortality in chickens in the study area.
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spelling pubmed-57612982018-01-17 Motile Salmonella serotypes causing high mortality in poultry farms in three South-Western States of Nigeria Mshelbwala, Fakilahyel Musa Ibrahim, Najume Doguwar-Giginya Saidu, Shehu NaAllah Azeez, Aminullah Ajiyobiojo Akinduti, Paul Akinniyi Kwanashie, Clara Nna Fakilahyel Kadiri, Amina Kinta Muhammed, Maryam Fagbamila, Idowu Oluwabunmi Luka, Pam Dachung Vet Rec Open Food/Farmed Animals This study was carried out to identify the Salmonella serotypes causing high mortality in chickens in Lagos, Ogun and Oyo states, Nigeria. Chickens presented for postmortem examination during disease outbreaks that were characterised by high mortality (40 per cent to 80 per cent) in poultry farms in the study area were examined from January to December, 2013. Samples of the lungs, heart, liver, spleen, kidneys, proventriculus, intestine and caecum were collected from suspected cases of salmonellosis, for bacterial culture and identification. Salmonella isolates were confirmed using PCR and serotyped using the Kauffman-White scheme. Twenty-six day-old pullets were raised to two weeks and inoculated orally with 0.2 mL of 1×10(8) colony forming units of Salmonella Zega identified in the present study to determine their pathogenicity, while another 26 served as control. The Salmonella serotypes were S Zega (n=13; 35.14 per cent), Salmonella Kentucky (n=9; 24.32 per cent), Salmonella Herston (n=6; 16.22 per cent), Salmonella Nima (n=4; 10.81 per cent), Salmonella Telelkebir (n=3; 8.11 per cent), Salmonella Colindale (n=1; 2.70 per cent) and Salmonella Tshiongwe (n=1; 2.70 per cent). Clinical signs in both natural and experimental infections were acute (70 per cent) and chronic (30 per cent), and included weakness, anorexia, yellowish diarrhoea, pasted vents, somnolescence and mortality, while gross lesions showed marked pulmonary congestion and oedema, necrotic foci in the myocardium; the liver, spleen and kidneys were markedly enlarged and had subcapsular multifocal necrosis. There were catarrhal proventriculitis and enteritis, and haemorrhagic typhlitis. While most of the serotypes identified in the present study have been isolated from poultry sources from commercial farms in Nigeria, to the best of the authors' knowledge, they have not been previously reported to cause high mortality in chickens in the study area. BMJ Publishing Group 2017-12-22 /pmc/articles/PMC5761298/ /pubmed/29344363 http://dx.doi.org/10.1136/vetreco-2017-000247 Text en © British Veterinary Association (unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted unless otherwise expressly granted. This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
spellingShingle Food/Farmed Animals
Mshelbwala, Fakilahyel Musa
Ibrahim, Najume Doguwar-Giginya
Saidu, Shehu NaAllah
Azeez, Aminullah Ajiyobiojo
Akinduti, Paul Akinniyi
Kwanashie, Clara Nna
Fakilahyel Kadiri, Amina Kinta
Muhammed, Maryam
Fagbamila, Idowu Oluwabunmi
Luka, Pam Dachung
Motile Salmonella serotypes causing high mortality in poultry farms in three South-Western States of Nigeria
title Motile Salmonella serotypes causing high mortality in poultry farms in three South-Western States of Nigeria
title_full Motile Salmonella serotypes causing high mortality in poultry farms in three South-Western States of Nigeria
title_fullStr Motile Salmonella serotypes causing high mortality in poultry farms in three South-Western States of Nigeria
title_full_unstemmed Motile Salmonella serotypes causing high mortality in poultry farms in three South-Western States of Nigeria
title_short Motile Salmonella serotypes causing high mortality in poultry farms in three South-Western States of Nigeria
title_sort motile salmonella serotypes causing high mortality in poultry farms in three south-western states of nigeria
topic Food/Farmed Animals
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761298/
https://www.ncbi.nlm.nih.gov/pubmed/29344363
http://dx.doi.org/10.1136/vetreco-2017-000247
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