Cargando…

PCB118-Induced Cell Proliferation Mediated by Oxidative Stress and MAPK Signaling Pathway in HELF Cells

The present study used human lung fibroblast (HELF) cells as a test model to evaluate the role of oxidative stress (OS) and extracellular signal-regulated kinases 1/2 (ERK1/2) protein in HELF cell proliferation exposed to PCB118. Results from 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bro...

Descripción completa

Detalles Bibliográficos
Autores principales: Hashmi, Muhammad Zaffar, Hasnain, Ahmad, Syed, Jabir Hussain, Tariq, Muhammad, Su, Xiaomei, Mubarak, Hussani, Nasim, Wajid, Shen, Chaofeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761904/
https://www.ncbi.nlm.nih.gov/pubmed/29344011
http://dx.doi.org/10.1177/1559325817751525
_version_ 1783291613313761280
author Hashmi, Muhammad Zaffar
Hasnain, Ahmad
Syed, Jabir Hussain
Tariq, Muhammad
Su, Xiaomei
Mubarak, Hussani
Nasim, Wajid
Shen, Chaofeng
author_facet Hashmi, Muhammad Zaffar
Hasnain, Ahmad
Syed, Jabir Hussain
Tariq, Muhammad
Su, Xiaomei
Mubarak, Hussani
Nasim, Wajid
Shen, Chaofeng
author_sort Hashmi, Muhammad Zaffar
collection PubMed
description The present study used human lung fibroblast (HELF) cells as a test model to evaluate the role of oxidative stress (OS) and extracellular signal-regulated kinases 1/2 (ERK1/2) protein in HELF cell proliferation exposed to PCB118. Results from 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide demonstrated that PCB118 at lower concentrations stimulated proliferation of HELF cell and abrogate proliferative effect at higher dose concentrations and in a time-dependent manner. Moreover, reactive oxygen species, malondialdehyde (MDA), and superoxide dismutase showed a significant increase at higher concentrations of PCB118 than the lower concentrations with the passage of time. Antioxidant enzymes such as glutathione peroxidase exhibited decreasing trends in dose- and time-dependent manner. Lipid peroxidation assay resulted in a significant increase in MDA level in PCB118-treated HELF cells compared with controls, suggesting that OS plays a key role in PCB118-induced toxicity. Comet assay indicated a significant increase in genotoxicity at higher concentrations of PCB118 exposure than the lower concentrations. It was found that PCB118 showed expression of ERK1/2 protein after 4 hours, while after 48 hours, the protein expression was less, indicating PCB toxicity to MAPK protein of HELF cell. Oxidative stress, ERK1/2, and HELF cell proliferation exhibited correlation. The results will elaborate toxicological evaluation of PCB118 to HELF cells and will help to develop drug for PCB-induced diseases.
format Online
Article
Text
id pubmed-5761904
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher SAGE Publications
record_format MEDLINE/PubMed
spelling pubmed-57619042018-01-17 PCB118-Induced Cell Proliferation Mediated by Oxidative Stress and MAPK Signaling Pathway in HELF Cells Hashmi, Muhammad Zaffar Hasnain, Ahmad Syed, Jabir Hussain Tariq, Muhammad Su, Xiaomei Mubarak, Hussani Nasim, Wajid Shen, Chaofeng Dose Response Original Article The present study used human lung fibroblast (HELF) cells as a test model to evaluate the role of oxidative stress (OS) and extracellular signal-regulated kinases 1/2 (ERK1/2) protein in HELF cell proliferation exposed to PCB118. Results from 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide demonstrated that PCB118 at lower concentrations stimulated proliferation of HELF cell and abrogate proliferative effect at higher dose concentrations and in a time-dependent manner. Moreover, reactive oxygen species, malondialdehyde (MDA), and superoxide dismutase showed a significant increase at higher concentrations of PCB118 than the lower concentrations with the passage of time. Antioxidant enzymes such as glutathione peroxidase exhibited decreasing trends in dose- and time-dependent manner. Lipid peroxidation assay resulted in a significant increase in MDA level in PCB118-treated HELF cells compared with controls, suggesting that OS plays a key role in PCB118-induced toxicity. Comet assay indicated a significant increase in genotoxicity at higher concentrations of PCB118 exposure than the lower concentrations. It was found that PCB118 showed expression of ERK1/2 protein after 4 hours, while after 48 hours, the protein expression was less, indicating PCB toxicity to MAPK protein of HELF cell. Oxidative stress, ERK1/2, and HELF cell proliferation exhibited correlation. The results will elaborate toxicological evaluation of PCB118 to HELF cells and will help to develop drug for PCB-induced diseases. SAGE Publications 2018-01-08 /pmc/articles/PMC5761904/ /pubmed/29344011 http://dx.doi.org/10.1177/1559325817751525 Text en © The Author(s) 2018 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Hashmi, Muhammad Zaffar
Hasnain, Ahmad
Syed, Jabir Hussain
Tariq, Muhammad
Su, Xiaomei
Mubarak, Hussani
Nasim, Wajid
Shen, Chaofeng
PCB118-Induced Cell Proliferation Mediated by Oxidative Stress and MAPK Signaling Pathway in HELF Cells
title PCB118-Induced Cell Proliferation Mediated by Oxidative Stress and MAPK Signaling Pathway in HELF Cells
title_full PCB118-Induced Cell Proliferation Mediated by Oxidative Stress and MAPK Signaling Pathway in HELF Cells
title_fullStr PCB118-Induced Cell Proliferation Mediated by Oxidative Stress and MAPK Signaling Pathway in HELF Cells
title_full_unstemmed PCB118-Induced Cell Proliferation Mediated by Oxidative Stress and MAPK Signaling Pathway in HELF Cells
title_short PCB118-Induced Cell Proliferation Mediated by Oxidative Stress and MAPK Signaling Pathway in HELF Cells
title_sort pcb118-induced cell proliferation mediated by oxidative stress and mapk signaling pathway in helf cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761904/
https://www.ncbi.nlm.nih.gov/pubmed/29344011
http://dx.doi.org/10.1177/1559325817751525
work_keys_str_mv AT hashmimuhammadzaffar pcb118inducedcellproliferationmediatedbyoxidativestressandmapksignalingpathwayinhelfcells
AT hasnainahmad pcb118inducedcellproliferationmediatedbyoxidativestressandmapksignalingpathwayinhelfcells
AT syedjabirhussain pcb118inducedcellproliferationmediatedbyoxidativestressandmapksignalingpathwayinhelfcells
AT tariqmuhammad pcb118inducedcellproliferationmediatedbyoxidativestressandmapksignalingpathwayinhelfcells
AT suxiaomei pcb118inducedcellproliferationmediatedbyoxidativestressandmapksignalingpathwayinhelfcells
AT mubarakhussani pcb118inducedcellproliferationmediatedbyoxidativestressandmapksignalingpathwayinhelfcells
AT nasimwajid pcb118inducedcellproliferationmediatedbyoxidativestressandmapksignalingpathwayinhelfcells
AT shenchaofeng pcb118inducedcellproliferationmediatedbyoxidativestressandmapksignalingpathwayinhelfcells