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The Combined Antitumor Effects of (125)I Radioactive Particle Implantation and Cytokine-Induced Killer Cell Therapy on Xenograft Hepatocellular Carcinoma in a Mouse Model

The combination of radiotherapy and immunotherapy has shown great promise in eradicating tumors. For example, (125)I radioactive particle implantation and cytokine-induced killer cell therapies have demonstrated efficacy in treating hepatocellular carcinoma. However, the mechanism of this combinatio...

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Autores principales: Zhang, Junyong, Wu, Nian, Lian, Zhengrong, Feng, Huyi, Jiang, Qing, Chen, Xianfeng, Gong, Jianping, Qiao, Zhengrong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762075/
https://www.ncbi.nlm.nih.gov/pubmed/29332456
http://dx.doi.org/10.1177/1533034617732204
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author Zhang, Junyong
Wu, Nian
Lian, Zhengrong
Feng, Huyi
Jiang, Qing
Chen, Xianfeng
Gong, Jianping
Qiao, Zhengrong
author_facet Zhang, Junyong
Wu, Nian
Lian, Zhengrong
Feng, Huyi
Jiang, Qing
Chen, Xianfeng
Gong, Jianping
Qiao, Zhengrong
author_sort Zhang, Junyong
collection PubMed
description The combination of radiotherapy and immunotherapy has shown great promise in eradicating tumors. For example, (125)I radioactive particle implantation and cytokine-induced killer cell therapies have demonstrated efficacy in treating hepatocellular carcinoma. However, the mechanism of this combination therapy remains unknown. In this study, we utilized cytokine-induced killer cells obtained from human peripheral blood mononuclear cells along with (125)I radioactive particle implantation to treat subcutaneous hepatocellular carcinoma xenograft tumors in BALB/c nude mice. The effects of combination therapy on tumor growth, tumor cell apoptosis and proliferation, animal survival, and immune indexes were then assessed. The results indicated that (125)I radioactive particle implantation combined with cytokine-induced killer cells shows a much greater antitumor therapeutic effect than either of the therapies alone when compared to control treatments. Mice treated with a combination of radiotherapy and immunotherapy displayed significantly reduced tumor growth. (125)I radioactive particle implantation upregulated the expression of major histocompatibility complex (MHC) class I chain-related gene A in hepatocellular carcinoma cells and enhanced cytokine-induced killer cell–mediated apoptosis through activation of caspase-3. Furthermore, cytokine-induced killer cells supplied immune substrates to induce a strong immune response after (125)I radioactive particle implantation therapy. In conclusion, (125)I radioactive particle implantation combined with cytokine-induced killer cell therapy significantly inhibits the growth of human hepatocellular carcinoma cells in vivo and improves animal survival times through mutual promotion of antitumor immunity, presenting a promising therapy for hepatocellular carcinoma.
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spelling pubmed-57620752018-01-17 The Combined Antitumor Effects of (125)I Radioactive Particle Implantation and Cytokine-Induced Killer Cell Therapy on Xenograft Hepatocellular Carcinoma in a Mouse Model Zhang, Junyong Wu, Nian Lian, Zhengrong Feng, Huyi Jiang, Qing Chen, Xianfeng Gong, Jianping Qiao, Zhengrong Technol Cancer Res Treat Original Articles The combination of radiotherapy and immunotherapy has shown great promise in eradicating tumors. For example, (125)I radioactive particle implantation and cytokine-induced killer cell therapies have demonstrated efficacy in treating hepatocellular carcinoma. However, the mechanism of this combination therapy remains unknown. In this study, we utilized cytokine-induced killer cells obtained from human peripheral blood mononuclear cells along with (125)I radioactive particle implantation to treat subcutaneous hepatocellular carcinoma xenograft tumors in BALB/c nude mice. The effects of combination therapy on tumor growth, tumor cell apoptosis and proliferation, animal survival, and immune indexes were then assessed. The results indicated that (125)I radioactive particle implantation combined with cytokine-induced killer cells shows a much greater antitumor therapeutic effect than either of the therapies alone when compared to control treatments. Mice treated with a combination of radiotherapy and immunotherapy displayed significantly reduced tumor growth. (125)I radioactive particle implantation upregulated the expression of major histocompatibility complex (MHC) class I chain-related gene A in hepatocellular carcinoma cells and enhanced cytokine-induced killer cell–mediated apoptosis through activation of caspase-3. Furthermore, cytokine-induced killer cells supplied immune substrates to induce a strong immune response after (125)I radioactive particle implantation therapy. In conclusion, (125)I radioactive particle implantation combined with cytokine-induced killer cell therapy significantly inhibits the growth of human hepatocellular carcinoma cells in vivo and improves animal survival times through mutual promotion of antitumor immunity, presenting a promising therapy for hepatocellular carcinoma. SAGE Publications 2017-09-25 2017-12 /pmc/articles/PMC5762075/ /pubmed/29332456 http://dx.doi.org/10.1177/1533034617732204 Text en © The Author(s) 2017 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Articles
Zhang, Junyong
Wu, Nian
Lian, Zhengrong
Feng, Huyi
Jiang, Qing
Chen, Xianfeng
Gong, Jianping
Qiao, Zhengrong
The Combined Antitumor Effects of (125)I Radioactive Particle Implantation and Cytokine-Induced Killer Cell Therapy on Xenograft Hepatocellular Carcinoma in a Mouse Model
title The Combined Antitumor Effects of (125)I Radioactive Particle Implantation and Cytokine-Induced Killer Cell Therapy on Xenograft Hepatocellular Carcinoma in a Mouse Model
title_full The Combined Antitumor Effects of (125)I Radioactive Particle Implantation and Cytokine-Induced Killer Cell Therapy on Xenograft Hepatocellular Carcinoma in a Mouse Model
title_fullStr The Combined Antitumor Effects of (125)I Radioactive Particle Implantation and Cytokine-Induced Killer Cell Therapy on Xenograft Hepatocellular Carcinoma in a Mouse Model
title_full_unstemmed The Combined Antitumor Effects of (125)I Radioactive Particle Implantation and Cytokine-Induced Killer Cell Therapy on Xenograft Hepatocellular Carcinoma in a Mouse Model
title_short The Combined Antitumor Effects of (125)I Radioactive Particle Implantation and Cytokine-Induced Killer Cell Therapy on Xenograft Hepatocellular Carcinoma in a Mouse Model
title_sort combined antitumor effects of (125)i radioactive particle implantation and cytokine-induced killer cell therapy on xenograft hepatocellular carcinoma in a mouse model
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762075/
https://www.ncbi.nlm.nih.gov/pubmed/29332456
http://dx.doi.org/10.1177/1533034617732204
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