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Optimization, validation and application of an assay for the activity of HMG-CoA reductase in vitro by LC–MS/MS()
A stable HMG-CoA reductase (HMGR) reaction in vitro was developed by a sensitive, selective and precise liquid chromatography–tandem mass spectrometry (LC–MS/MS) method. The optimized enzyme reaction condition contained 1.5 μg of HMGR, 20 nM of NADPH with 50 min of reaction time. The method was vali...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Xi'an Jiaotong University
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762244/ https://www.ncbi.nlm.nih.gov/pubmed/29403953 http://dx.doi.org/10.1016/j.jpha.2015.06.002 |