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IGF-1 induces the epithelial-mesenchymal transition via Stat5 in hepatocellular carcinoma
It has been reported that the epithelial-mesenchymal transition (EMT) plays an important role in hepatocellular carcinoma (HCC). However, the relationship between the insulin-like growth factor-1 (IGF-1) and EMT of HCC was not fully elucidated. In the present work, we found that the expression of N-...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762369/ https://www.ncbi.nlm.nih.gov/pubmed/29340101 http://dx.doi.org/10.18632/oncotarget.22952 |
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author | Zhao, Chuanzong Wang, Qian Wang, Ben Sun, Qi He, Zhaobin Hong, Jianguo Kuehn, Florian Liu, Enyu Zhang, Zongli |
author_facet | Zhao, Chuanzong Wang, Qian Wang, Ben Sun, Qi He, Zhaobin Hong, Jianguo Kuehn, Florian Liu, Enyu Zhang, Zongli |
author_sort | Zhao, Chuanzong |
collection | PubMed |
description | It has been reported that the epithelial-mesenchymal transition (EMT) plays an important role in hepatocellular carcinoma (HCC). However, the relationship between the insulin-like growth factor-1 (IGF-1) and EMT of HCC was not fully elucidated. In the present work, we found that the expression of N-cadherin, Vimentin, Snail1, Snail2, and Twist1 was positively associated with IGF-1R expression, while E-cadherin expression was negatively associated with IGF-1 expression in human HCC samples. Furthermore, we observed that IGF-1 up-regulated the expression of N-cadherin, Vimentin, Snail1, Snail2 and Twist1, and down-regulated the expression of E-cadherin. In addition, Stat5 was induced in IGF-1-treated HepG2 and Hep3B cells, and Stat5 inhibition or siRNA significantly affected IGF-1-induced EMT in HepG2 and Hep3B cells. In conclusion, IGF-1 induces EMT of HCC via Stat5 signaling pathway. Thus, IGF-1/Stat5 can be recommended as a potential and novel therapeutic strategy for HCC patients. |
format | Online Article Text |
id | pubmed-5762369 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-57623692018-01-16 IGF-1 induces the epithelial-mesenchymal transition via Stat5 in hepatocellular carcinoma Zhao, Chuanzong Wang, Qian Wang, Ben Sun, Qi He, Zhaobin Hong, Jianguo Kuehn, Florian Liu, Enyu Zhang, Zongli Oncotarget Research Paper It has been reported that the epithelial-mesenchymal transition (EMT) plays an important role in hepatocellular carcinoma (HCC). However, the relationship between the insulin-like growth factor-1 (IGF-1) and EMT of HCC was not fully elucidated. In the present work, we found that the expression of N-cadherin, Vimentin, Snail1, Snail2, and Twist1 was positively associated with IGF-1R expression, while E-cadherin expression was negatively associated with IGF-1 expression in human HCC samples. Furthermore, we observed that IGF-1 up-regulated the expression of N-cadherin, Vimentin, Snail1, Snail2 and Twist1, and down-regulated the expression of E-cadherin. In addition, Stat5 was induced in IGF-1-treated HepG2 and Hep3B cells, and Stat5 inhibition or siRNA significantly affected IGF-1-induced EMT in HepG2 and Hep3B cells. In conclusion, IGF-1 induces EMT of HCC via Stat5 signaling pathway. Thus, IGF-1/Stat5 can be recommended as a potential and novel therapeutic strategy for HCC patients. Impact Journals LLC 2017-12-05 /pmc/articles/PMC5762369/ /pubmed/29340101 http://dx.doi.org/10.18632/oncotarget.22952 Text en Copyright: © 2017 Zhao et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Zhao, Chuanzong Wang, Qian Wang, Ben Sun, Qi He, Zhaobin Hong, Jianguo Kuehn, Florian Liu, Enyu Zhang, Zongli IGF-1 induces the epithelial-mesenchymal transition via Stat5 in hepatocellular carcinoma |
title | IGF-1 induces the epithelial-mesenchymal transition via Stat5 in hepatocellular carcinoma |
title_full | IGF-1 induces the epithelial-mesenchymal transition via Stat5 in hepatocellular carcinoma |
title_fullStr | IGF-1 induces the epithelial-mesenchymal transition via Stat5 in hepatocellular carcinoma |
title_full_unstemmed | IGF-1 induces the epithelial-mesenchymal transition via Stat5 in hepatocellular carcinoma |
title_short | IGF-1 induces the epithelial-mesenchymal transition via Stat5 in hepatocellular carcinoma |
title_sort | igf-1 induces the epithelial-mesenchymal transition via stat5 in hepatocellular carcinoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762369/ https://www.ncbi.nlm.nih.gov/pubmed/29340101 http://dx.doi.org/10.18632/oncotarget.22952 |
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