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LC–HRMS determination of piperine on rat dried blood spots: A pharmacokinetic study()
A liquid chromatography–high resolution mass spectrometry (LC–HRMS) method was developed and validated for the determination of piperine (PPR) on dried blood spots (DBS). DBS samples were prepared by spiking the whole blood with analyte to produce 30 µL of blood spots on specimen collection cards. C...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Xi'an Jiaotong University
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762438/ https://www.ncbi.nlm.nih.gov/pubmed/29403958 http://dx.doi.org/10.1016/j.jpha.2015.07.002 |
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author | Ramesh, Bokka Rao Vadaparthi, P. Rajesh Sukumar, Genji Manjula, Nemali Suresh Babu, Katragadda Sita Devi, Potturi |
author_facet | Ramesh, Bokka Rao Vadaparthi, P. Rajesh Sukumar, Genji Manjula, Nemali Suresh Babu, Katragadda Sita Devi, Potturi |
author_sort | Ramesh, Bokka |
collection | PubMed |
description | A liquid chromatography–high resolution mass spectrometry (LC–HRMS) method was developed and validated for the determination of piperine (PPR) on dried blood spots (DBS). DBS samples were prepared by spiking the whole blood with analyte to produce 30 µL of blood spots on specimen collection cards. Chromatographic separation was achieved on an Atlantis dC(18) column using acetonitrile and water (0.1% formic acid) (85:15, v/v) as mobile phase in an isocratic mode of elution at a flow rate of 0.75 mL/min. MS detection was carried out in electrospray positive ion mode for the target ions and monitored at m/z 286.1465 for PPR and 272.1303 for the internal standard (IS). The developed method exhibited a linear dynamic range over 0.01–2000 ng/mL for PPR on DBS. The overall extraction recovery of PPR from DBS was 92.5%. Influence of hematocrit and spot volume on DBS was also evaluated and found to be well within the acceptable limits. The method was successfully applied to pharmacokinetic studies of PPR in rats. |
format | Online Article Text |
id | pubmed-5762438 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Xi'an Jiaotong University |
record_format | MEDLINE/PubMed |
spelling | pubmed-57624382018-02-05 LC–HRMS determination of piperine on rat dried blood spots: A pharmacokinetic study() Ramesh, Bokka Rao Vadaparthi, P. Rajesh Sukumar, Genji Manjula, Nemali Suresh Babu, Katragadda Sita Devi, Potturi J Pharm Anal Original Article A liquid chromatography–high resolution mass spectrometry (LC–HRMS) method was developed and validated for the determination of piperine (PPR) on dried blood spots (DBS). DBS samples were prepared by spiking the whole blood with analyte to produce 30 µL of blood spots on specimen collection cards. Chromatographic separation was achieved on an Atlantis dC(18) column using acetonitrile and water (0.1% formic acid) (85:15, v/v) as mobile phase in an isocratic mode of elution at a flow rate of 0.75 mL/min. MS detection was carried out in electrospray positive ion mode for the target ions and monitored at m/z 286.1465 for PPR and 272.1303 for the internal standard (IS). The developed method exhibited a linear dynamic range over 0.01–2000 ng/mL for PPR on DBS. The overall extraction recovery of PPR from DBS was 92.5%. Influence of hematocrit and spot volume on DBS was also evaluated and found to be well within the acceptable limits. The method was successfully applied to pharmacokinetic studies of PPR in rats. Xi'an Jiaotong University 2016-02 2015-07-20 /pmc/articles/PMC5762438/ /pubmed/29403958 http://dx.doi.org/10.1016/j.jpha.2015.07.002 Text en © 2015 Xi'an Jiaotong University. Production and hosting by Elsevier B.V. All rights reserved. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Ramesh, Bokka Rao Vadaparthi, P. Rajesh Sukumar, Genji Manjula, Nemali Suresh Babu, Katragadda Sita Devi, Potturi LC–HRMS determination of piperine on rat dried blood spots: A pharmacokinetic study() |
title | LC–HRMS determination of piperine on rat dried blood spots: A pharmacokinetic study() |
title_full | LC–HRMS determination of piperine on rat dried blood spots: A pharmacokinetic study() |
title_fullStr | LC–HRMS determination of piperine on rat dried blood spots: A pharmacokinetic study() |
title_full_unstemmed | LC–HRMS determination of piperine on rat dried blood spots: A pharmacokinetic study() |
title_short | LC–HRMS determination of piperine on rat dried blood spots: A pharmacokinetic study() |
title_sort | lc–hrms determination of piperine on rat dried blood spots: a pharmacokinetic study() |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762438/ https://www.ncbi.nlm.nih.gov/pubmed/29403958 http://dx.doi.org/10.1016/j.jpha.2015.07.002 |
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