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Cryptic transmission of ST405 Escherichia coli carrying bla(NDM-4) in hospital
Three carbapenem-resistant Escherichia coli were recovered from rectal swabs of different patients in a tertiary hospital and were found carrying bla (NDM-4,) an uncommon bla (NDM) variant. Genome sequences of the isolates were obtained using Illumina technology and the long-read MinION sequencer. T...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762679/ https://www.ncbi.nlm.nih.gov/pubmed/29321680 http://dx.doi.org/10.1038/s41598-017-18910-w |
Sumario: | Three carbapenem-resistant Escherichia coli were recovered from rectal swabs of different patients in a tertiary hospital and were found carrying bla (NDM-4,) an uncommon bla (NDM) variant. Genome sequences of the isolates were obtained using Illumina technology and the long-read MinION sequencer. The isolates belonged to ST405 and phylogenetic group D, a globally distributed lineage associated with antimicrobial resistance. In addition to bla (NDM-4), the three isolates carried 14 known resistance genes including the extended-spectrum β-lactamase gene bla (CTX-M-15). There were only 1 or 2 SNPs between the isolates, suggesting a common origin and cryptic transmission in hospital. bla (NDM-4) was located on a 46.5-kb IncFIA self-transmissible plasmid, which may facilitate further dissemination of bla (NDM-4). Two copies of IS26 bracketed a 14.6-kb region containing bla (NDM-4) and have the potential to form a composite transposon for mediating the mobilization of bla (NDM-4). |
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