Cryptic transmission of ST405 Escherichia coli carrying bla(NDM-4) in hospital

Three carbapenem-resistant Escherichia coli were recovered from rectal swabs of different patients in a tertiary hospital and were found carrying bla (NDM-4,) an uncommon bla (NDM) variant. Genome sequences of the isolates were obtained using Illumina technology and the long-read MinION sequencer. The isolates belonged to ST405 and phylogenetic group D, a globally distributed lineage associated with antimicrobial resistance. In addition to bla (NDM-4), the three isolates carried 14 known resistance genes including the extended-spectrum β-lactamase gene bla (CTX-M-15). There were only 1 or 2 SNPs between the isolates, suggesting a common origin and cryptic transmission in hospital. bla (NDM-4) was located on a 46.5-kb IncFIA self-transmissible plasmid, which may facilitate further dissemination of bla (NDM-4). Two copies of IS26 bracketed a 14.6-kb region containing bla (NDM-4) and have the potential to form a composite transposon for mediating the mobilization of bla (NDM-4).