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Transfer of a bla(CTX-M-1)-carrying plasmid between different Escherichia coli strains within the human gut explored by whole genome sequencing analyses
Horizontal transfer of antibiotic resistance determinants contributes to dissemination of antibiotic resistance. Such transfer of resistance genes within the human gut has been documented in some in vivo studies. The present study investigated seven bla (CTX-M-1)-carrying Escherichia coli isolates f...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762863/ https://www.ncbi.nlm.nih.gov/pubmed/29321570 http://dx.doi.org/10.1038/s41598-017-18659-2 |
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author | Knudsen, Per Kristian Gammelsrud, Karianne Wiger Alfsnes, Kristian Steinbakk, Martin Abrahamsen, Tore G. Müller, Fredrik Bohlin, Jon |
author_facet | Knudsen, Per Kristian Gammelsrud, Karianne Wiger Alfsnes, Kristian Steinbakk, Martin Abrahamsen, Tore G. Müller, Fredrik Bohlin, Jon |
author_sort | Knudsen, Per Kristian |
collection | PubMed |
description | Horizontal transfer of antibiotic resistance determinants contributes to dissemination of antibiotic resistance. Such transfer of resistance genes within the human gut has been documented in some in vivo studies. The present study investigated seven bla (CTX-M-1)-carrying Escherichia coli isolates from three consecutive faecal samples collected from one cystic fibrosis patient in a nine-months period, by analysing whole genome sequencing data. The analyses showed that the seven E. coli isolates represented three genetically diverse strains. All isolates contained bla (CTX-M-1)-carrying Incl1 plasmids that shared a common 101 kb backbone differing by only four SNPs. The plasmids harboured by the three different E. coli strains varied within limited regions suggestive of recombination events, according to the phylogenetic topology of the genomes of the isolates harbouring them. The findings strongly suggest that horizontal transfer of a bla (CTX-M-1)-carrying plasmid had occurred within the patient´s gut. The study illustrates the within-host diversity of faecally carried resistant E. coli isolates and highlights the value of collecting multiple bacterial colonies from longitudinally collected samples to assess faecal carriage of resistant enterobacteria. The clustering of the plasmids with the corresponding E. coli strains carrying them indicates that the plasmids appear to have adapted to their respective E. coli hosts. |
format | Online Article Text |
id | pubmed-5762863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57628632018-01-17 Transfer of a bla(CTX-M-1)-carrying plasmid between different Escherichia coli strains within the human gut explored by whole genome sequencing analyses Knudsen, Per Kristian Gammelsrud, Karianne Wiger Alfsnes, Kristian Steinbakk, Martin Abrahamsen, Tore G. Müller, Fredrik Bohlin, Jon Sci Rep Article Horizontal transfer of antibiotic resistance determinants contributes to dissemination of antibiotic resistance. Such transfer of resistance genes within the human gut has been documented in some in vivo studies. The present study investigated seven bla (CTX-M-1)-carrying Escherichia coli isolates from three consecutive faecal samples collected from one cystic fibrosis patient in a nine-months period, by analysing whole genome sequencing data. The analyses showed that the seven E. coli isolates represented three genetically diverse strains. All isolates contained bla (CTX-M-1)-carrying Incl1 plasmids that shared a common 101 kb backbone differing by only four SNPs. The plasmids harboured by the three different E. coli strains varied within limited regions suggestive of recombination events, according to the phylogenetic topology of the genomes of the isolates harbouring them. The findings strongly suggest that horizontal transfer of a bla (CTX-M-1)-carrying plasmid had occurred within the patient´s gut. The study illustrates the within-host diversity of faecally carried resistant E. coli isolates and highlights the value of collecting multiple bacterial colonies from longitudinally collected samples to assess faecal carriage of resistant enterobacteria. The clustering of the plasmids with the corresponding E. coli strains carrying them indicates that the plasmids appear to have adapted to their respective E. coli hosts. Nature Publishing Group UK 2018-01-10 /pmc/articles/PMC5762863/ /pubmed/29321570 http://dx.doi.org/10.1038/s41598-017-18659-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Knudsen, Per Kristian Gammelsrud, Karianne Wiger Alfsnes, Kristian Steinbakk, Martin Abrahamsen, Tore G. Müller, Fredrik Bohlin, Jon Transfer of a bla(CTX-M-1)-carrying plasmid between different Escherichia coli strains within the human gut explored by whole genome sequencing analyses |
title | Transfer of a bla(CTX-M-1)-carrying plasmid between different Escherichia coli strains within the human gut explored by whole genome sequencing analyses |
title_full | Transfer of a bla(CTX-M-1)-carrying plasmid between different Escherichia coli strains within the human gut explored by whole genome sequencing analyses |
title_fullStr | Transfer of a bla(CTX-M-1)-carrying plasmid between different Escherichia coli strains within the human gut explored by whole genome sequencing analyses |
title_full_unstemmed | Transfer of a bla(CTX-M-1)-carrying plasmid between different Escherichia coli strains within the human gut explored by whole genome sequencing analyses |
title_short | Transfer of a bla(CTX-M-1)-carrying plasmid between different Escherichia coli strains within the human gut explored by whole genome sequencing analyses |
title_sort | transfer of a bla(ctx-m-1)-carrying plasmid between different escherichia coli strains within the human gut explored by whole genome sequencing analyses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762863/ https://www.ncbi.nlm.nih.gov/pubmed/29321570 http://dx.doi.org/10.1038/s41598-017-18659-2 |
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