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In vivo monitoring of hair cycle stages via bioluminescence imaging of hair follicle NG2 cells
Hair growth occurs periodically in a cycle that consists of three different phases: growth, regression, and resting. The length of each phase is regulated by both intrinsic and extrinsic factors throughout life, and influenced by physiological and pathological conditions. Elongation of the resting p...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762894/ https://www.ncbi.nlm.nih.gov/pubmed/29321681 http://dx.doi.org/10.1038/s41598-017-18763-3 |
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author | Tamura, Yasuhisa Takata, Kumi Eguchi, Asami Kataoka, Yosky |
author_facet | Tamura, Yasuhisa Takata, Kumi Eguchi, Asami Kataoka, Yosky |
author_sort | Tamura, Yasuhisa |
collection | PubMed |
description | Hair growth occurs periodically in a cycle that consists of three different phases: growth, regression, and resting. The length of each phase is regulated by both intrinsic and extrinsic factors throughout life, and influenced by physiological and pathological conditions. Elongation of the resting phase and shortening of the growth phase occur during physiological ageing and in baldness, respectively. In vivo discrimination of each phase of the hair cycle can be used to research for regeneration of hair follicles as well as to evaluate the efficacy of hair regrowth treatments in the same individual. Here we show that NG2+ epithelial cells in the hair follicles encompass bulge stem cells, and that the number of hair follicle NG2 cells underwent dramatic changes during the hair cycle. Transgenic rats with expression of firefly luciferase gene in NG2 cells were generated to monitor the hair cycle in vivo. Hair follicle NG2 cells were clearly visualized via bioluminescence imaging to study each phase of the hair cycle in the rats, from infancy to old age. |
format | Online Article Text |
id | pubmed-5762894 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57628942018-01-17 In vivo monitoring of hair cycle stages via bioluminescence imaging of hair follicle NG2 cells Tamura, Yasuhisa Takata, Kumi Eguchi, Asami Kataoka, Yosky Sci Rep Article Hair growth occurs periodically in a cycle that consists of three different phases: growth, regression, and resting. The length of each phase is regulated by both intrinsic and extrinsic factors throughout life, and influenced by physiological and pathological conditions. Elongation of the resting phase and shortening of the growth phase occur during physiological ageing and in baldness, respectively. In vivo discrimination of each phase of the hair cycle can be used to research for regeneration of hair follicles as well as to evaluate the efficacy of hair regrowth treatments in the same individual. Here we show that NG2+ epithelial cells in the hair follicles encompass bulge stem cells, and that the number of hair follicle NG2 cells underwent dramatic changes during the hair cycle. Transgenic rats with expression of firefly luciferase gene in NG2 cells were generated to monitor the hair cycle in vivo. Hair follicle NG2 cells were clearly visualized via bioluminescence imaging to study each phase of the hair cycle in the rats, from infancy to old age. Nature Publishing Group UK 2018-01-10 /pmc/articles/PMC5762894/ /pubmed/29321681 http://dx.doi.org/10.1038/s41598-017-18763-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Tamura, Yasuhisa Takata, Kumi Eguchi, Asami Kataoka, Yosky In vivo monitoring of hair cycle stages via bioluminescence imaging of hair follicle NG2 cells |
title | In vivo monitoring of hair cycle stages via bioluminescence imaging of hair follicle NG2 cells |
title_full | In vivo monitoring of hair cycle stages via bioluminescence imaging of hair follicle NG2 cells |
title_fullStr | In vivo monitoring of hair cycle stages via bioluminescence imaging of hair follicle NG2 cells |
title_full_unstemmed | In vivo monitoring of hair cycle stages via bioluminescence imaging of hair follicle NG2 cells |
title_short | In vivo monitoring of hair cycle stages via bioluminescence imaging of hair follicle NG2 cells |
title_sort | in vivo monitoring of hair cycle stages via bioluminescence imaging of hair follicle ng2 cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762894/ https://www.ncbi.nlm.nih.gov/pubmed/29321681 http://dx.doi.org/10.1038/s41598-017-18763-3 |
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