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Telomeric G-Quadruplexes: From Human to Tetrahymena Repeats

The human telomeric and protozoal telomeric sequences differ only in one purine base in their repeats; TTAGGG in telomeric sequences; and TTGGGG in protozoal sequences. In this study, the relationship between G-quadruplexes formed from these repeats and their derivatives is analyzed and compared. Th...

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Autores principales: Demkovičová, Erika, Bauer, Ľuboš, Krafčíková, Petra, Tlučková, Katarína, Tóthova, Petra, Halaganová, Andrea, Valušová, Eva, Víglaský, Viktor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5763100/
https://www.ncbi.nlm.nih.gov/pubmed/29445544
http://dx.doi.org/10.1155/2017/9170371
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author Demkovičová, Erika
Bauer, Ľuboš
Krafčíková, Petra
Tlučková, Katarína
Tóthova, Petra
Halaganová, Andrea
Valušová, Eva
Víglaský, Viktor
author_facet Demkovičová, Erika
Bauer, Ľuboš
Krafčíková, Petra
Tlučková, Katarína
Tóthova, Petra
Halaganová, Andrea
Valušová, Eva
Víglaský, Viktor
author_sort Demkovičová, Erika
collection PubMed
description The human telomeric and protozoal telomeric sequences differ only in one purine base in their repeats; TTAGGG in telomeric sequences; and TTGGGG in protozoal sequences. In this study, the relationship between G-quadruplexes formed from these repeats and their derivatives is analyzed and compared. The human telomeric DNA sequence G(3)(T(2)AG(3))(3) and related sequences in which each adenine base has been systematically replaced by a guanine were investigated; the result is Tetrahymena repeats. The substitution does not affect the formation of G-quadruplexes but may cause differences in topology. The results also show that the stability of the substituted derivatives increased in sequences with greater number of substitutions. In addition, most of the sequences containing imperfections in repeats which were analyzed in this study also occur in human and Tetrahymena genomes. Generally, the presence of G-quadruplex structures in any organism is a source of limitations during the life cycle. Therefore, a fuller understanding of the influence of base substitution on the structural variability of G-quadruplexes would be of considerable scientific value.
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spelling pubmed-57631002018-02-14 Telomeric G-Quadruplexes: From Human to Tetrahymena Repeats Demkovičová, Erika Bauer, Ľuboš Krafčíková, Petra Tlučková, Katarína Tóthova, Petra Halaganová, Andrea Valušová, Eva Víglaský, Viktor J Nucleic Acids Research Article The human telomeric and protozoal telomeric sequences differ only in one purine base in their repeats; TTAGGG in telomeric sequences; and TTGGGG in protozoal sequences. In this study, the relationship between G-quadruplexes formed from these repeats and their derivatives is analyzed and compared. The human telomeric DNA sequence G(3)(T(2)AG(3))(3) and related sequences in which each adenine base has been systematically replaced by a guanine were investigated; the result is Tetrahymena repeats. The substitution does not affect the formation of G-quadruplexes but may cause differences in topology. The results also show that the stability of the substituted derivatives increased in sequences with greater number of substitutions. In addition, most of the sequences containing imperfections in repeats which were analyzed in this study also occur in human and Tetrahymena genomes. Generally, the presence of G-quadruplex structures in any organism is a source of limitations during the life cycle. Therefore, a fuller understanding of the influence of base substitution on the structural variability of G-quadruplexes would be of considerable scientific value. Hindawi 2017 2017-12-28 /pmc/articles/PMC5763100/ /pubmed/29445544 http://dx.doi.org/10.1155/2017/9170371 Text en Copyright © 2017 Erika Demkovičová et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Demkovičová, Erika
Bauer, Ľuboš
Krafčíková, Petra
Tlučková, Katarína
Tóthova, Petra
Halaganová, Andrea
Valušová, Eva
Víglaský, Viktor
Telomeric G-Quadruplexes: From Human to Tetrahymena Repeats
title Telomeric G-Quadruplexes: From Human to Tetrahymena Repeats
title_full Telomeric G-Quadruplexes: From Human to Tetrahymena Repeats
title_fullStr Telomeric G-Quadruplexes: From Human to Tetrahymena Repeats
title_full_unstemmed Telomeric G-Quadruplexes: From Human to Tetrahymena Repeats
title_short Telomeric G-Quadruplexes: From Human to Tetrahymena Repeats
title_sort telomeric g-quadruplexes: from human to tetrahymena repeats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5763100/
https://www.ncbi.nlm.nih.gov/pubmed/29445544
http://dx.doi.org/10.1155/2017/9170371
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