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Early Events in Populus Hybrid and Fagus sylvatica Leaves Exposed to Ozone

This paper aims to investigate early responses to ozone in leaves of Fagus sylvatica (beech) and Populus maximowiczii x Populus berolinensis (poplar). The experimental setup consisted of four open-air (OA) plots, four charcoal-filtered (CF) open-top chambers (OTCs), and four nonfiltered (NF) OTCs. Q...

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Detalles Bibliográficos
Autores principales: Desotgiu, R., Bussotti, F., Faoro, F., Iriti, M., Agati, G., Marzuoli, R., Gerosa, G., Tani, C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5763675/
https://www.ncbi.nlm.nih.gov/pubmed/20364237
http://dx.doi.org/10.1100/tsw.2010.63
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author Desotgiu, R.
Bussotti, F.
Faoro, F.
Iriti, M.
Agati, G.
Marzuoli, R.
Gerosa, G.
Tani, C.
author_facet Desotgiu, R.
Bussotti, F.
Faoro, F.
Iriti, M.
Agati, G.
Marzuoli, R.
Gerosa, G.
Tani, C.
author_sort Desotgiu, R.
collection PubMed
description This paper aims to investigate early responses to ozone in leaves of Fagus sylvatica (beech) and Populus maximowiczii x Populus berolinensis (poplar). The experimental setup consisted of four open-air (OA) plots, four charcoal-filtered (CF) open-top chambers (OTCs), and four nonfiltered (NF) OTCs. Qualitative and quantitative analyses were carried out on nonsymptomatic (CF) and symptomatic (NF and OA) leaves of both species. Qualitative analyses were performed applying microscopic techniques: Evans blue staining for detection of cell viability, CeCl(3) staining of transmission electron microscope (TEM) samples to detect the accumulation of H(2)O(2), and multispectral fluorescence microimaging and microspectrofluorometry to investigate the accumulation of fluorescent phenolic compounds in the walls of the damaged cells. Quantitative analyses consisted of the analysis of the chlorophyll a fluorescence transients (fast kinetics). The early responses to ozone were demonstrated by the Evans blue and CeCl(3) staining techniques that provided evidence of plant responses in both species 1 month before foliar symptoms became visible. The fluorescence transients analysis, too, demonstrated the breakdown of the oxygen evolving system and the inactivation of the end receptors of electrons at a very early stage, both in poplar and in beech. The accumulation of phenolic compounds in the cell walls, on the other hand, was a species-specific response detected in poplar, but not in beech. Evans blue and CeCl(3) staining, as well as the multispectral fluorescence microimaging and microspectrofluorometry, can be used to support the field diagnosis of ozone injury, whereas the fast kinetics of chlorophyll fluorescence provides evidence of early physiological responses.
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spelling pubmed-57636752018-06-03 Early Events in Populus Hybrid and Fagus sylvatica Leaves Exposed to Ozone Desotgiu, R. Bussotti, F. Faoro, F. Iriti, M. Agati, G. Marzuoli, R. Gerosa, G. Tani, C. ScientificWorldJournal Research Article This paper aims to investigate early responses to ozone in leaves of Fagus sylvatica (beech) and Populus maximowiczii x Populus berolinensis (poplar). The experimental setup consisted of four open-air (OA) plots, four charcoal-filtered (CF) open-top chambers (OTCs), and four nonfiltered (NF) OTCs. Qualitative and quantitative analyses were carried out on nonsymptomatic (CF) and symptomatic (NF and OA) leaves of both species. Qualitative analyses were performed applying microscopic techniques: Evans blue staining for detection of cell viability, CeCl(3) staining of transmission electron microscope (TEM) samples to detect the accumulation of H(2)O(2), and multispectral fluorescence microimaging and microspectrofluorometry to investigate the accumulation of fluorescent phenolic compounds in the walls of the damaged cells. Quantitative analyses consisted of the analysis of the chlorophyll a fluorescence transients (fast kinetics). The early responses to ozone were demonstrated by the Evans blue and CeCl(3) staining techniques that provided evidence of plant responses in both species 1 month before foliar symptoms became visible. The fluorescence transients analysis, too, demonstrated the breakdown of the oxygen evolving system and the inactivation of the end receptors of electrons at a very early stage, both in poplar and in beech. The accumulation of phenolic compounds in the cell walls, on the other hand, was a species-specific response detected in poplar, but not in beech. Evans blue and CeCl(3) staining, as well as the multispectral fluorescence microimaging and microspectrofluorometry, can be used to support the field diagnosis of ozone injury, whereas the fast kinetics of chlorophyll fluorescence provides evidence of early physiological responses. TheScientificWorldJOURNAL 2010-04-01 /pmc/articles/PMC5763675/ /pubmed/20364237 http://dx.doi.org/10.1100/tsw.2010.63 Text en Copyright © 2010 R. Desotgiu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Desotgiu, R.
Bussotti, F.
Faoro, F.
Iriti, M.
Agati, G.
Marzuoli, R.
Gerosa, G.
Tani, C.
Early Events in Populus Hybrid and Fagus sylvatica Leaves Exposed to Ozone
title Early Events in Populus Hybrid and Fagus sylvatica Leaves Exposed to Ozone
title_full Early Events in Populus Hybrid and Fagus sylvatica Leaves Exposed to Ozone
title_fullStr Early Events in Populus Hybrid and Fagus sylvatica Leaves Exposed to Ozone
title_full_unstemmed Early Events in Populus Hybrid and Fagus sylvatica Leaves Exposed to Ozone
title_short Early Events in Populus Hybrid and Fagus sylvatica Leaves Exposed to Ozone
title_sort early events in populus hybrid and fagus sylvatica leaves exposed to ozone
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5763675/
https://www.ncbi.nlm.nih.gov/pubmed/20364237
http://dx.doi.org/10.1100/tsw.2010.63
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