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DNA Evidence Uncompromised by Active Oxygen

Currently, forensic sciences can make use of the potential of instrumental analysis techniques to obtain information from the smallest, even invisible, samples. However, as laboratory techniques improve, so too should the procedures applied in the search for and initial testing of clues in order to...

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Detalles Bibliográficos
Autores principales: Castelló, Ana, Francés, Francesc, Verdú, Fernando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5763935/
https://www.ncbi.nlm.nih.gov/pubmed/20209384
http://dx.doi.org/10.1100/tsw.2010.47
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author Castelló, Ana
Francés, Francesc
Verdú, Fernando
author_facet Castelló, Ana
Francés, Francesc
Verdú, Fernando
author_sort Castelló, Ana
collection PubMed
description Currently, forensic sciences can make use of the potential of instrumental analysis techniques to obtain information from the smallest, even invisible, samples. However, as laboratory techniques improve, so too should the procedures applied in the search for and initial testing of clues in order to be equally effective. This requires continuous revision so that those procedures may resolve the problems that samples present. As far as bloodstains are concerned, there are methods available that are recognized as being both highly sensitive and effective. Nevertheless, the marketing of new cleaning products, those that contain active oxygen, has raised doubts about the ability of those procedures to detect blood. It has been shown that stains washed with these detergents (and still visible) invalidated both the presumptive test (reduced phenolphthalein, luminol, and Bluestar®) and that applied for determining human hemoglobin. These findings have caused considerable concern both within the forensic and scientific community, and among the general public, so obliging us to seek solutions. In this work, the effect of these new cleaning products on DNA analyses is studied. The results, encouraging ones, show that these detergents, despite invalidating all other tests, do not hinder the extraction, or the subsequent analysis, of DNA.
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spelling pubmed-57639352018-06-03 DNA Evidence Uncompromised by Active Oxygen Castelló, Ana Francés, Francesc Verdú, Fernando ScientificWorldJournal Research Article Currently, forensic sciences can make use of the potential of instrumental analysis techniques to obtain information from the smallest, even invisible, samples. However, as laboratory techniques improve, so too should the procedures applied in the search for and initial testing of clues in order to be equally effective. This requires continuous revision so that those procedures may resolve the problems that samples present. As far as bloodstains are concerned, there are methods available that are recognized as being both highly sensitive and effective. Nevertheless, the marketing of new cleaning products, those that contain active oxygen, has raised doubts about the ability of those procedures to detect blood. It has been shown that stains washed with these detergents (and still visible) invalidated both the presumptive test (reduced phenolphthalein, luminol, and Bluestar®) and that applied for determining human hemoglobin. These findings have caused considerable concern both within the forensic and scientific community, and among the general public, so obliging us to seek solutions. In this work, the effect of these new cleaning products on DNA analyses is studied. The results, encouraging ones, show that these detergents, despite invalidating all other tests, do not hinder the extraction, or the subsequent analysis, of DNA. TheScientificWorldJOURNAL 2010-03-05 /pmc/articles/PMC5763935/ /pubmed/20209384 http://dx.doi.org/10.1100/tsw.2010.47 Text en Copyright © 2010 Ana Castelló et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Castelló, Ana
Francés, Francesc
Verdú, Fernando
DNA Evidence Uncompromised by Active Oxygen
title DNA Evidence Uncompromised by Active Oxygen
title_full DNA Evidence Uncompromised by Active Oxygen
title_fullStr DNA Evidence Uncompromised by Active Oxygen
title_full_unstemmed DNA Evidence Uncompromised by Active Oxygen
title_short DNA Evidence Uncompromised by Active Oxygen
title_sort dna evidence uncompromised by active oxygen
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5763935/
https://www.ncbi.nlm.nih.gov/pubmed/20209384
http://dx.doi.org/10.1100/tsw.2010.47
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