Activation and Genetic Modification of Human Monocyte-Derived Dendritic Cells using Attenuated Salmonella typhimurium

Live attenuated bacterial vectors, such as Salmonella typhimurium, have shown promise as delivery vehicles for DNA. We have examined two new strains of S. typhimurium and their impact on dendritic cell maturation (CD12-sifA/aroC mutant and WT05-ssaV/aroC, both in TML background). Strain WT05 matured...

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Autores principales: Michael, Agnieszka, John, Justin, Meyer, Brendan, Pandha, Hardev
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5763986/
https://www.ncbi.nlm.nih.gov/pubmed/20209385
http://dx.doi.org/10.1100/tsw.2010.37
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author Michael, Agnieszka
John, Justin
Meyer, Brendan
Pandha, Hardev
author_facet Michael, Agnieszka
John, Justin
Meyer, Brendan
Pandha, Hardev
author_sort Michael, Agnieszka
collection PubMed
description Live attenuated bacterial vectors, such as Salmonella typhimurium, have shown promise as delivery vehicles for DNA. We have examined two new strains of S. typhimurium and their impact on dendritic cell maturation (CD12-sifA/aroC mutant and WT05-ssaV/aroC, both in TML background). Strain WT05 matured dendritic cells in a more efficient way; caused higher release of cytokines TNF-α, IL-12, IL-1β; and was efficient for gene transfer. These findings suggest that the genetic background of the attenuation can influence the pattern of inflammatory immune response to Salmonella infection.
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spelling pubmed-57639862018-06-03 Activation and Genetic Modification of Human Monocyte-Derived Dendritic Cells using Attenuated Salmonella typhimurium Michael, Agnieszka John, Justin Meyer, Brendan Pandha, Hardev ScientificWorldJournal Research Article Live attenuated bacterial vectors, such as Salmonella typhimurium, have shown promise as delivery vehicles for DNA. We have examined two new strains of S. typhimurium and their impact on dendritic cell maturation (CD12-sifA/aroC mutant and WT05-ssaV/aroC, both in TML background). Strain WT05 matured dendritic cells in a more efficient way; caused higher release of cytokines TNF-α, IL-12, IL-1β; and was efficient for gene transfer. These findings suggest that the genetic background of the attenuation can influence the pattern of inflammatory immune response to Salmonella infection. TheScientificWorldJOURNAL 2010-03-05 /pmc/articles/PMC5763986/ /pubmed/20209385 http://dx.doi.org/10.1100/tsw.2010.37 Text en Copyright © 2010 Agnieszka Michael et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Michael, Agnieszka
John, Justin
Meyer, Brendan
Pandha, Hardev
Activation and Genetic Modification of Human Monocyte-Derived Dendritic Cells using Attenuated Salmonella typhimurium
title Activation and Genetic Modification of Human Monocyte-Derived Dendritic Cells using Attenuated Salmonella typhimurium
title_full Activation and Genetic Modification of Human Monocyte-Derived Dendritic Cells using Attenuated Salmonella typhimurium
title_fullStr Activation and Genetic Modification of Human Monocyte-Derived Dendritic Cells using Attenuated Salmonella typhimurium
title_full_unstemmed Activation and Genetic Modification of Human Monocyte-Derived Dendritic Cells using Attenuated Salmonella typhimurium
title_short Activation and Genetic Modification of Human Monocyte-Derived Dendritic Cells using Attenuated Salmonella typhimurium
title_sort activation and genetic modification of human monocyte-derived dendritic cells using attenuated salmonella typhimurium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5763986/
https://www.ncbi.nlm.nih.gov/pubmed/20209385
http://dx.doi.org/10.1100/tsw.2010.37
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