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EDAG promotes the expansion and survival of human CD34(+) cells
EDAG is multifunctional transcriptional regulator primarily expressed in the lin(lo)c(-)kit(+)Sca-1(+) hematopoietic stem cells (HSC) and CD34(+) progenitor cells. Previous studies indicate that EDAG is required for maintaining hematopoietic lineage commitment balance. Here using ex vivo culture and...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5764277/ https://www.ncbi.nlm.nih.gov/pubmed/29324880 http://dx.doi.org/10.1371/journal.pone.0190794 |
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author | Zhao, Ke Zheng, Wei-Wei Dong, Xiao-Ming Yin, Rong-Hua Gao, Rui Li, Xiu Liu, Jin-Fang Zhan, Yi-Qun Yu, Miao Chen, Hui Ge, Chang-Hui Ning, Hong-Mei Yang, Xiao-Ming Li, Chang-Yan |
author_facet | Zhao, Ke Zheng, Wei-Wei Dong, Xiao-Ming Yin, Rong-Hua Gao, Rui Li, Xiu Liu, Jin-Fang Zhan, Yi-Qun Yu, Miao Chen, Hui Ge, Chang-Hui Ning, Hong-Mei Yang, Xiao-Ming Li, Chang-Yan |
author_sort | Zhao, Ke |
collection | PubMed |
description | EDAG is multifunctional transcriptional regulator primarily expressed in the lin(lo)c(-)kit(+)Sca-1(+) hematopoietic stem cells (HSC) and CD34(+) progenitor cells. Previous studies indicate that EDAG is required for maintaining hematopoietic lineage commitment balance. Here using ex vivo culture and HSC transplantation models, we report that EDAG enhances the proliferative potential of human cord blood CD34(+) cells, increases survival, prevents cell apoptosis and promotes their repopulating capacity. Moreover, EDAG overexpression induces rapid entry of CD34(+) cells into the cell cycle. Gene expression profile analysis indicate that EDAG knockdown leads to down-regulation of various positive cell cycle regulators including cyclin A, B, D, and E. Together these data provides novel insights into EDAG in regulation of expansion and survival of human hematopoietic stem/progenitor cells. |
format | Online Article Text |
id | pubmed-5764277 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-57642772018-01-23 EDAG promotes the expansion and survival of human CD34(+) cells Zhao, Ke Zheng, Wei-Wei Dong, Xiao-Ming Yin, Rong-Hua Gao, Rui Li, Xiu Liu, Jin-Fang Zhan, Yi-Qun Yu, Miao Chen, Hui Ge, Chang-Hui Ning, Hong-Mei Yang, Xiao-Ming Li, Chang-Yan PLoS One Research Article EDAG is multifunctional transcriptional regulator primarily expressed in the lin(lo)c(-)kit(+)Sca-1(+) hematopoietic stem cells (HSC) and CD34(+) progenitor cells. Previous studies indicate that EDAG is required for maintaining hematopoietic lineage commitment balance. Here using ex vivo culture and HSC transplantation models, we report that EDAG enhances the proliferative potential of human cord blood CD34(+) cells, increases survival, prevents cell apoptosis and promotes their repopulating capacity. Moreover, EDAG overexpression induces rapid entry of CD34(+) cells into the cell cycle. Gene expression profile analysis indicate that EDAG knockdown leads to down-regulation of various positive cell cycle regulators including cyclin A, B, D, and E. Together these data provides novel insights into EDAG in regulation of expansion and survival of human hematopoietic stem/progenitor cells. Public Library of Science 2018-01-11 /pmc/articles/PMC5764277/ /pubmed/29324880 http://dx.doi.org/10.1371/journal.pone.0190794 Text en © 2018 Zhao et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zhao, Ke Zheng, Wei-Wei Dong, Xiao-Ming Yin, Rong-Hua Gao, Rui Li, Xiu Liu, Jin-Fang Zhan, Yi-Qun Yu, Miao Chen, Hui Ge, Chang-Hui Ning, Hong-Mei Yang, Xiao-Ming Li, Chang-Yan EDAG promotes the expansion and survival of human CD34(+) cells |
title | EDAG promotes the expansion and survival of human CD34(+) cells |
title_full | EDAG promotes the expansion and survival of human CD34(+) cells |
title_fullStr | EDAG promotes the expansion and survival of human CD34(+) cells |
title_full_unstemmed | EDAG promotes the expansion and survival of human CD34(+) cells |
title_short | EDAG promotes the expansion and survival of human CD34(+) cells |
title_sort | edag promotes the expansion and survival of human cd34(+) cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5764277/ https://www.ncbi.nlm.nih.gov/pubmed/29324880 http://dx.doi.org/10.1371/journal.pone.0190794 |
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