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MicroRNA-939 inhibits cell proliferation via targeting LRSAM1 in Hirschsprung’s disease

BACKGROUND: Hirschsprung's disease (HSCR) is a common digestive disease caused by impaired development of neural crest cells. Some studies have revealed the roles of microRNA (miRNA) in various diseases. But the function of miRNA in HSCR needs further investigation. METHODS: We adopted qRT-PCR...

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Autores principales: Chen, Guanglin, Du, Chunxia, Shen, Ziyang, Peng, Lei, Xie, Hua, Zang, Rujin, Li, Hongxing, Xia, Yankai, Tang, Weibing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5764386/
https://www.ncbi.nlm.nih.gov/pubmed/29253842
http://dx.doi.org/10.18632/aging.101331
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author Chen, Guanglin
Du, Chunxia
Shen, Ziyang
Peng, Lei
Xie, Hua
Zang, Rujin
Li, Hongxing
Xia, Yankai
Tang, Weibing
author_facet Chen, Guanglin
Du, Chunxia
Shen, Ziyang
Peng, Lei
Xie, Hua
Zang, Rujin
Li, Hongxing
Xia, Yankai
Tang, Weibing
author_sort Chen, Guanglin
collection PubMed
description BACKGROUND: Hirschsprung's disease (HSCR) is a common digestive disease caused by impaired development of neural crest cells. Some studies have revealed the roles of microRNA (miRNA) in various diseases. But the function of miRNA in HSCR needs further investigation. METHODS: We adopted qRT-PCR and immunoblot analyses to explore the relative expression of miR-939 and LRSAM1 in 80 HSCR bowel tissues and 80 normal bowel tissues. CCK-8 assay, transwell assay and flow cytometry were used to evaluate the function of miR-939 by overexpression of miR-939 in 293T, SK-N-BE(2), SH-SY5Y cell lines. The direct connection between miR-939 and LRSAM1 was validated by dual-luciferase reporter assay. We also investigated the autophagy level via immunoblot analyses. RESULTS: Mir-939 was significantly upregulated in HSCR tissues with decreased expression of LRSAM1. Overexpression of miR-939 suppressed cell proliferation without affecting cell apoptosis, cell cycle or cell migration. And LRSAM1 exerted similar function. Autophagy was impaired in HSCR tissues compared with control samples. Mir-939 did not inhibit the autophagy although it decreased the expression of LRSAM1. CONCLUSIONS: Our study shows the potential function of mir-939 through regulating LRSAM1 in HSCR and infers that autophagy may also confer the risk of HSCR.
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spelling pubmed-57643862018-01-14 MicroRNA-939 inhibits cell proliferation via targeting LRSAM1 in Hirschsprung’s disease Chen, Guanglin Du, Chunxia Shen, Ziyang Peng, Lei Xie, Hua Zang, Rujin Li, Hongxing Xia, Yankai Tang, Weibing Aging (Albany NY) Research Paper BACKGROUND: Hirschsprung's disease (HSCR) is a common digestive disease caused by impaired development of neural crest cells. Some studies have revealed the roles of microRNA (miRNA) in various diseases. But the function of miRNA in HSCR needs further investigation. METHODS: We adopted qRT-PCR and immunoblot analyses to explore the relative expression of miR-939 and LRSAM1 in 80 HSCR bowel tissues and 80 normal bowel tissues. CCK-8 assay, transwell assay and flow cytometry were used to evaluate the function of miR-939 by overexpression of miR-939 in 293T, SK-N-BE(2), SH-SY5Y cell lines. The direct connection between miR-939 and LRSAM1 was validated by dual-luciferase reporter assay. We also investigated the autophagy level via immunoblot analyses. RESULTS: Mir-939 was significantly upregulated in HSCR tissues with decreased expression of LRSAM1. Overexpression of miR-939 suppressed cell proliferation without affecting cell apoptosis, cell cycle or cell migration. And LRSAM1 exerted similar function. Autophagy was impaired in HSCR tissues compared with control samples. Mir-939 did not inhibit the autophagy although it decreased the expression of LRSAM1. CONCLUSIONS: Our study shows the potential function of mir-939 through regulating LRSAM1 in HSCR and infers that autophagy may also confer the risk of HSCR. Impact Journals 2017-12-18 /pmc/articles/PMC5764386/ /pubmed/29253842 http://dx.doi.org/10.18632/aging.101331 Text en Copyright © 2018 Chen et al http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Chen, Guanglin
Du, Chunxia
Shen, Ziyang
Peng, Lei
Xie, Hua
Zang, Rujin
Li, Hongxing
Xia, Yankai
Tang, Weibing
MicroRNA-939 inhibits cell proliferation via targeting LRSAM1 in Hirschsprung’s disease
title MicroRNA-939 inhibits cell proliferation via targeting LRSAM1 in Hirschsprung’s disease
title_full MicroRNA-939 inhibits cell proliferation via targeting LRSAM1 in Hirschsprung’s disease
title_fullStr MicroRNA-939 inhibits cell proliferation via targeting LRSAM1 in Hirschsprung’s disease
title_full_unstemmed MicroRNA-939 inhibits cell proliferation via targeting LRSAM1 in Hirschsprung’s disease
title_short MicroRNA-939 inhibits cell proliferation via targeting LRSAM1 in Hirschsprung’s disease
title_sort microrna-939 inhibits cell proliferation via targeting lrsam1 in hirschsprung’s disease
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5764386/
https://www.ncbi.nlm.nih.gov/pubmed/29253842
http://dx.doi.org/10.18632/aging.101331
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