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Expression of cry2Ah1 and two domain II mutants in transgenic tobacco confers high resistance to susceptible and Cry1Ac-resistant cotton bollworm

To improve the novel Bacillus thuringiensis insecticidal gene cry2Ah1 toxicity, two mutants cry2Ah1-vp (V354VP) and cry2Ah1-sp (V354SP) were performed. SWISS-MODEL analysis showed two mutants had a longer loop located between β-4 and β-5 of domain II, resulting in higher binding affinity with brush...

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Detalles Bibliográficos
Autores principales: Li, Shengyan, Wang, Zeyu, Zhou, Yiyao, Li, Changhui, Wang, Guiping, Wang, Hai, Zhang, Jie, Liang, Gemei, Lang, Zhihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765002/
https://www.ncbi.nlm.nih.gov/pubmed/29323243
http://dx.doi.org/10.1038/s41598-017-19064-5

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