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Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells

Hitherto, the known mechanisms underpinning cell-fate specification act on neural progenitors, affecting their commitment to generate neuron or glial cells. Here, we show that particular phospholipids supplemented in the culture media modify the commitment of post-mitotic neural cells in vitro. Phos...

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Autores principales: Montaner, Aneley, da Silva Santana, Themis Taynah, Schroeder, Timm, Einicker-Lamas, Marcelo, Girardini, Javier, Costa, Marcos Romualdo, Banchio, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765016/
https://www.ncbi.nlm.nih.gov/pubmed/29323239
http://dx.doi.org/10.1038/s41598-017-18700-4
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author Montaner, Aneley
da Silva Santana, Themis Taynah
Schroeder, Timm
Einicker-Lamas, Marcelo
Girardini, Javier
Costa, Marcos Romualdo
Banchio, Claudia
author_facet Montaner, Aneley
da Silva Santana, Themis Taynah
Schroeder, Timm
Einicker-Lamas, Marcelo
Girardini, Javier
Costa, Marcos Romualdo
Banchio, Claudia
author_sort Montaner, Aneley
collection PubMed
description Hitherto, the known mechanisms underpinning cell-fate specification act on neural progenitors, affecting their commitment to generate neuron or glial cells. Here, we show that particular phospholipids supplemented in the culture media modify the commitment of post-mitotic neural cells in vitro. Phosphatidylcholine (PtdCho)-enriched media enhances neuronal differentiation at the expense of astroglial and unspecified cells. Conversely, phosphatidylethanolamine (PtdEtn) enhances astroglial differentiation and accelerates astrocyte maturation. The ability of phospholipids to modify the fate of post-mitotic cells depends on its presence during a narrow time-window during cell differentiation and it is mediated by the selective activation of particular signaling pathways. While PtdCho-mediated effect on neuronal differentiation depends on cAMP-dependent kinase (PKA)/calcium responsive element binding protein (CREB), PtdEtn stimulates astrogliogenesis through the activation of the MEK/ERK signaling pathway. Collectively, our results provide an additional degree of plasticity in neural cell specification and further support the notion that cell differentiation is a reversible phenomenon. They also contribute to our understanding of neuronal and glial lineage specification in the central nervous system, opening up new avenues to retrieve neurogenic capacity in the brain.
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spelling pubmed-57650162018-01-17 Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells Montaner, Aneley da Silva Santana, Themis Taynah Schroeder, Timm Einicker-Lamas, Marcelo Girardini, Javier Costa, Marcos Romualdo Banchio, Claudia Sci Rep Article Hitherto, the known mechanisms underpinning cell-fate specification act on neural progenitors, affecting their commitment to generate neuron or glial cells. Here, we show that particular phospholipids supplemented in the culture media modify the commitment of post-mitotic neural cells in vitro. Phosphatidylcholine (PtdCho)-enriched media enhances neuronal differentiation at the expense of astroglial and unspecified cells. Conversely, phosphatidylethanolamine (PtdEtn) enhances astroglial differentiation and accelerates astrocyte maturation. The ability of phospholipids to modify the fate of post-mitotic cells depends on its presence during a narrow time-window during cell differentiation and it is mediated by the selective activation of particular signaling pathways. While PtdCho-mediated effect on neuronal differentiation depends on cAMP-dependent kinase (PKA)/calcium responsive element binding protein (CREB), PtdEtn stimulates astrogliogenesis through the activation of the MEK/ERK signaling pathway. Collectively, our results provide an additional degree of plasticity in neural cell specification and further support the notion that cell differentiation is a reversible phenomenon. They also contribute to our understanding of neuronal and glial lineage specification in the central nervous system, opening up new avenues to retrieve neurogenic capacity in the brain. Nature Publishing Group UK 2018-01-11 /pmc/articles/PMC5765016/ /pubmed/29323239 http://dx.doi.org/10.1038/s41598-017-18700-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Montaner, Aneley
da Silva Santana, Themis Taynah
Schroeder, Timm
Einicker-Lamas, Marcelo
Girardini, Javier
Costa, Marcos Romualdo
Banchio, Claudia
Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells
title Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells
title_full Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells
title_fullStr Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells
title_full_unstemmed Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells
title_short Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells
title_sort specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765016/
https://www.ncbi.nlm.nih.gov/pubmed/29323239
http://dx.doi.org/10.1038/s41598-017-18700-4
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