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Caveolin 1 Promotes Renal Water and Salt Reabsorption
Caveolin-1 (Cav1) is essential for the formation of caveolae. Little is known about their functional role in the kidney. We tested the hypothesis that caveolae modulate renal salt and water reabsorption. Wild-type (WT) and Cav1-deficient (Cav1−/−) mice were studied. Cav1 expression and caveolae form...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765123/ https://www.ncbi.nlm.nih.gov/pubmed/29323234 http://dx.doi.org/10.1038/s41598-017-19071-6 |
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author | Willière, Yan Borschewski, Aljona Patzak, Andreas Nikitina, Tatiana Dittmayer, Carsten Daigeler, Anna L. Schuelke, Markus Bachmann, Sebastian Mutig, Kerim |
author_facet | Willière, Yan Borschewski, Aljona Patzak, Andreas Nikitina, Tatiana Dittmayer, Carsten Daigeler, Anna L. Schuelke, Markus Bachmann, Sebastian Mutig, Kerim |
author_sort | Willière, Yan |
collection | PubMed |
description | Caveolin-1 (Cav1) is essential for the formation of caveolae. Little is known about their functional role in the kidney. We tested the hypothesis that caveolae modulate renal salt and water reabsorption. Wild-type (WT) and Cav1-deficient (Cav1−/−) mice were studied. Cav1 expression and caveolae formation were present in vascular cells, late distal convoluted tubule and principal connecting tubule and collecting duct cells of WT but not Cav1−/− kidneys. Urinary sodium excretion was increased by 94% and urine flow by 126% in Cav1−/− mice (p < 0.05). A decrease in activating phosphorylation of the Na-Cl cotransporter (NCC) of the distal convoluted tubule was recorded in Cav1−/− compared to WT kidneys (−40%; p < 0.05). Isolated intrarenal arteries from Cav1−/− mice revealed a fourfold reduction in sensitivity to phenylephrine (p < 0.05). A significantly diminished maximal contractile response (−13%; p < 0.05) was suggestive of enhanced nitric oxide (NO) availability. In line with this, the abundance of endothelial NO synthase (eNOS) was increased in Cav1−/− kidneys +213%; p < 0.05) and cultured caveolae-deprived cells showed intracellular accumulation of eNOS, compared to caveolae-intact controls. Our results suggest that renal caveolae help to conserve water and electrolytes via modulation of NCC function and regulation of vascular eNOS. |
format | Online Article Text |
id | pubmed-5765123 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57651232018-01-17 Caveolin 1 Promotes Renal Water and Salt Reabsorption Willière, Yan Borschewski, Aljona Patzak, Andreas Nikitina, Tatiana Dittmayer, Carsten Daigeler, Anna L. Schuelke, Markus Bachmann, Sebastian Mutig, Kerim Sci Rep Article Caveolin-1 (Cav1) is essential for the formation of caveolae. Little is known about their functional role in the kidney. We tested the hypothesis that caveolae modulate renal salt and water reabsorption. Wild-type (WT) and Cav1-deficient (Cav1−/−) mice were studied. Cav1 expression and caveolae formation were present in vascular cells, late distal convoluted tubule and principal connecting tubule and collecting duct cells of WT but not Cav1−/− kidneys. Urinary sodium excretion was increased by 94% and urine flow by 126% in Cav1−/− mice (p < 0.05). A decrease in activating phosphorylation of the Na-Cl cotransporter (NCC) of the distal convoluted tubule was recorded in Cav1−/− compared to WT kidneys (−40%; p < 0.05). Isolated intrarenal arteries from Cav1−/− mice revealed a fourfold reduction in sensitivity to phenylephrine (p < 0.05). A significantly diminished maximal contractile response (−13%; p < 0.05) was suggestive of enhanced nitric oxide (NO) availability. In line with this, the abundance of endothelial NO synthase (eNOS) was increased in Cav1−/− kidneys +213%; p < 0.05) and cultured caveolae-deprived cells showed intracellular accumulation of eNOS, compared to caveolae-intact controls. Our results suggest that renal caveolae help to conserve water and electrolytes via modulation of NCC function and regulation of vascular eNOS. Nature Publishing Group UK 2018-01-11 /pmc/articles/PMC5765123/ /pubmed/29323234 http://dx.doi.org/10.1038/s41598-017-19071-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Willière, Yan Borschewski, Aljona Patzak, Andreas Nikitina, Tatiana Dittmayer, Carsten Daigeler, Anna L. Schuelke, Markus Bachmann, Sebastian Mutig, Kerim Caveolin 1 Promotes Renal Water and Salt Reabsorption |
title | Caveolin 1 Promotes Renal Water and Salt Reabsorption |
title_full | Caveolin 1 Promotes Renal Water and Salt Reabsorption |
title_fullStr | Caveolin 1 Promotes Renal Water and Salt Reabsorption |
title_full_unstemmed | Caveolin 1 Promotes Renal Water and Salt Reabsorption |
title_short | Caveolin 1 Promotes Renal Water and Salt Reabsorption |
title_sort | caveolin 1 promotes renal water and salt reabsorption |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765123/ https://www.ncbi.nlm.nih.gov/pubmed/29323234 http://dx.doi.org/10.1038/s41598-017-19071-6 |
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