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Composition of salivary microbiota in elderly subjects

Frailty is gaining attention worldwide with the aging of society. Despite the potential lethality and multiple signs and symptoms in affected individuals, preclinical detection of early manifestations leading to frailty syndrome have not been established. We speculated that the composition of the or...

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Autores principales: Ogawa, Taiji, Hirose, Yujiro, Honda-Ogawa, Mariko, Sugimoto, Minami, Sasaki, Satoshi, Kibi, Masahito, Kawabata, Shigetada, Ikebe, Kazunori, Maeda, Yoshinobu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765146/
https://www.ncbi.nlm.nih.gov/pubmed/29323208
http://dx.doi.org/10.1038/s41598-017-18677-0
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author Ogawa, Taiji
Hirose, Yujiro
Honda-Ogawa, Mariko
Sugimoto, Minami
Sasaki, Satoshi
Kibi, Masahito
Kawabata, Shigetada
Ikebe, Kazunori
Maeda, Yoshinobu
author_facet Ogawa, Taiji
Hirose, Yujiro
Honda-Ogawa, Mariko
Sugimoto, Minami
Sasaki, Satoshi
Kibi, Masahito
Kawabata, Shigetada
Ikebe, Kazunori
Maeda, Yoshinobu
author_sort Ogawa, Taiji
collection PubMed
description Frailty is gaining attention worldwide with the aging of society. Despite the potential lethality and multiple signs and symptoms in affected individuals, preclinical detection of early manifestations leading to frailty syndrome have not been established. We speculated that the composition of the oral microbiota is associated with general frailty, as well as a relationship between gut microbiota and general health condition. In the present study, we investigated the salivary microbiota composition in samples from healthy and frail elderly individuals using 16S rRNA sequencing analysis for characterization. We found a significant difference in diversity between elderly individuals living in a nursing home (EN) and healthy control (HC) subjects, as well as in the microbiota composition at the phyla level. A supervised orthogonal partial least squared discriminant analysis (OPLS-DA) revealed a significant difference in clear classification trend between the EN and HC groups, with all observations falling within the Hotellings T(2) (0.95) ellipse, with model fitness parameters of R (2)(cum) = 0.937 and Q (2)(cum) = 0.888, respectively. In addition, the score plots by unsupervised principal component analysis (PCA) showed a clear classification trend in both groups. Our findings suggest that general frailty is associated with oral microbiota composition and formation.
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spelling pubmed-57651462018-01-17 Composition of salivary microbiota in elderly subjects Ogawa, Taiji Hirose, Yujiro Honda-Ogawa, Mariko Sugimoto, Minami Sasaki, Satoshi Kibi, Masahito Kawabata, Shigetada Ikebe, Kazunori Maeda, Yoshinobu Sci Rep Article Frailty is gaining attention worldwide with the aging of society. Despite the potential lethality and multiple signs and symptoms in affected individuals, preclinical detection of early manifestations leading to frailty syndrome have not been established. We speculated that the composition of the oral microbiota is associated with general frailty, as well as a relationship between gut microbiota and general health condition. In the present study, we investigated the salivary microbiota composition in samples from healthy and frail elderly individuals using 16S rRNA sequencing analysis for characterization. We found a significant difference in diversity between elderly individuals living in a nursing home (EN) and healthy control (HC) subjects, as well as in the microbiota composition at the phyla level. A supervised orthogonal partial least squared discriminant analysis (OPLS-DA) revealed a significant difference in clear classification trend between the EN and HC groups, with all observations falling within the Hotellings T(2) (0.95) ellipse, with model fitness parameters of R (2)(cum) = 0.937 and Q (2)(cum) = 0.888, respectively. In addition, the score plots by unsupervised principal component analysis (PCA) showed a clear classification trend in both groups. Our findings suggest that general frailty is associated with oral microbiota composition and formation. Nature Publishing Group UK 2018-01-11 /pmc/articles/PMC5765146/ /pubmed/29323208 http://dx.doi.org/10.1038/s41598-017-18677-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ogawa, Taiji
Hirose, Yujiro
Honda-Ogawa, Mariko
Sugimoto, Minami
Sasaki, Satoshi
Kibi, Masahito
Kawabata, Shigetada
Ikebe, Kazunori
Maeda, Yoshinobu
Composition of salivary microbiota in elderly subjects
title Composition of salivary microbiota in elderly subjects
title_full Composition of salivary microbiota in elderly subjects
title_fullStr Composition of salivary microbiota in elderly subjects
title_full_unstemmed Composition of salivary microbiota in elderly subjects
title_short Composition of salivary microbiota in elderly subjects
title_sort composition of salivary microbiota in elderly subjects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765146/
https://www.ncbi.nlm.nih.gov/pubmed/29323208
http://dx.doi.org/10.1038/s41598-017-18677-0
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