Validation of Anti-CSPα, SNAP25, Tyrosine Hydroxylase, Ubiquitin, Cleaved Caspase 3, and pSer PKC Motif Antibodies for Utilization in Western Blotting

There are many commercial antibodies with little information provided by their suppliers as to their reliability. Accordingly, commercial antibodies require proper validation before being used in scientific research. In this study, we validated several commercial antibodies, including anti-CSPα, SNA...

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Autores principales: Shirafuji, Toshihiko, Ueyama, Takehiko, Tanaka, Shigeru, Hide, Izumi, Saito, Naoaki, Sakai, Norio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY 2017
Materias:
Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765218/
https://www.ncbi.nlm.nih.gov/pubmed/29343881
http://dx.doi.org/10.1267/ahc.17028
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author Shirafuji, Toshihiko
Ueyama, Takehiko
Tanaka, Shigeru
Hide, Izumi
Saito, Naoaki
Sakai, Norio
author_facet Shirafuji, Toshihiko
Ueyama, Takehiko
Tanaka, Shigeru
Hide, Izumi
Saito, Naoaki
Sakai, Norio
author_sort Shirafuji, Toshihiko
collection PubMed
description There are many commercial antibodies with little information provided by their suppliers as to their reliability. Accordingly, commercial antibodies require proper validation before being used in scientific research. In this study, we validated several commercial antibodies, including anti-CSPα, SNAP25, tyrosine hydroxylase, ubiquitin, cleaved caspase 3, and pSer PKC motif. Anti-CSPα, SNAP25, and tyrosine hydroxylase antibodies could detect their endogenous target proteins with some degree of cross-reactivity. Furthermore, clear SNAP25 staining was observed with SNAP25 antibody. Antibodies directed against ubiquitin, cleaved caspase 3, and pSer PKC motif could detect poly-ubiquitination, apoptosis, and phosphorylation, respectively.
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spelling pubmed-57652182018-01-17 Validation of Anti-CSPα, SNAP25, Tyrosine Hydroxylase, Ubiquitin, Cleaved Caspase 3, and pSer PKC Motif Antibodies for Utilization in Western Blotting Shirafuji, Toshihiko Ueyama, Takehiko Tanaka, Shigeru Hide, Izumi Saito, Naoaki Sakai, Norio Acta Histochem Cytochem Note There are many commercial antibodies with little information provided by their suppliers as to their reliability. Accordingly, commercial antibodies require proper validation before being used in scientific research. In this study, we validated several commercial antibodies, including anti-CSPα, SNAP25, tyrosine hydroxylase, ubiquitin, cleaved caspase 3, and pSer PKC motif. Anti-CSPα, SNAP25, and tyrosine hydroxylase antibodies could detect their endogenous target proteins with some degree of cross-reactivity. Furthermore, clear SNAP25 staining was observed with SNAP25 antibody. Antibodies directed against ubiquitin, cleaved caspase 3, and pSer PKC motif could detect poly-ubiquitination, apoptosis, and phosphorylation, respectively. JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY 2017-12-26 2017-12-21 /pmc/articles/PMC5765218/ /pubmed/29343881 http://dx.doi.org/10.1267/ahc.17028 Text en 2017 The Japan Society of Histochemistry and Cytochemistry This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Note
Shirafuji, Toshihiko
Ueyama, Takehiko
Tanaka, Shigeru
Hide, Izumi
Saito, Naoaki
Sakai, Norio
Validation of Anti-CSPα, SNAP25, Tyrosine Hydroxylase, Ubiquitin, Cleaved Caspase 3, and pSer PKC Motif Antibodies for Utilization in Western Blotting
title Validation of Anti-CSPα, SNAP25, Tyrosine Hydroxylase, Ubiquitin, Cleaved Caspase 3, and pSer PKC Motif Antibodies for Utilization in Western Blotting
title_full Validation of Anti-CSPα, SNAP25, Tyrosine Hydroxylase, Ubiquitin, Cleaved Caspase 3, and pSer PKC Motif Antibodies for Utilization in Western Blotting
title_fullStr Validation of Anti-CSPα, SNAP25, Tyrosine Hydroxylase, Ubiquitin, Cleaved Caspase 3, and pSer PKC Motif Antibodies for Utilization in Western Blotting
title_full_unstemmed Validation of Anti-CSPα, SNAP25, Tyrosine Hydroxylase, Ubiquitin, Cleaved Caspase 3, and pSer PKC Motif Antibodies for Utilization in Western Blotting
title_short Validation of Anti-CSPα, SNAP25, Tyrosine Hydroxylase, Ubiquitin, Cleaved Caspase 3, and pSer PKC Motif Antibodies for Utilization in Western Blotting
title_sort validation of anti-cspα, snap25, tyrosine hydroxylase, ubiquitin, cleaved caspase 3, and pser pkc motif antibodies for utilization in western blotting
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765218/
https://www.ncbi.nlm.nih.gov/pubmed/29343881
http://dx.doi.org/10.1267/ahc.17028
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