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Downregulation of CD24 suppresses bone metastasis of lung cancer

Suppression of bone metastasis can improve patient quality of life. Current drugs for bone metastasis have been shown to prolong progression‐free survival but not overall survival; therefore, other potential therapeutic targets for bone metastasis should be investigated. Cell‐surface antigens, such...

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Autores principales: Okabe, Hinako, Aoki, Katsuhiko, Yogosawa, Satomi, Saito, Mitsuru, Marumo, Keishi, Yoshida, Kiyotsugu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765300/
https://www.ncbi.nlm.nih.gov/pubmed/29095550
http://dx.doi.org/10.1111/cas.13435
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author Okabe, Hinako
Aoki, Katsuhiko
Yogosawa, Satomi
Saito, Mitsuru
Marumo, Keishi
Yoshida, Kiyotsugu
author_facet Okabe, Hinako
Aoki, Katsuhiko
Yogosawa, Satomi
Saito, Mitsuru
Marumo, Keishi
Yoshida, Kiyotsugu
author_sort Okabe, Hinako
collection PubMed
description Suppression of bone metastasis can improve patient quality of life. Current drugs for bone metastasis have been shown to prolong progression‐free survival but not overall survival; therefore, other potential therapeutic targets for bone metastasis should be investigated. Cell‐surface antigens, such as CD24, have been recently shown to be involved in the metastasis of various cancers. However, whether CD24 plays a role in bone metastasis of lung cancer remains unknown. To observe metastasis of lung cancer cells by imaging technology, we introduced a near‐infrared fluorescent protein, iRFP720, into a bone‐seeking subclone established from lung cancer cells, HARA‐B4 cells. The anchorage‐independent growth of these cells was then evaluated by colony formation assays. We also compared cancer cell tropism to bone tissue with HARA‐B4 cells in the presence or absence of CD24 by cell adhesion assays. To clarify the role of CD24 in bone metastasis, we intracardially injected CD24‐knockdown HARA‐B4 cells into mice and monitored metastasis through detection of iRFP720 using an in vivo imaging system. CD24‐knockdown HARA‐B4 cells in vitro showed reduced anchorage‐independent growth and cancer cell tropism to bone. Bone metastasis was diminished in mice inoculated with CD24‐knockdown HARA‐B4 cells, which was rescued by add‐back of CD24 in cells. Our findings indicate that iRFP720 is effective for in vivo imaging analysis of bone metastasis and that downregulation of CD24 suppresses bone metastasis of lung cancer cells. These findings collectively indicate that CD24 may be considered a promising new therapeutic candidate for the prevention of bone metastasis of lung cancer.
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spelling pubmed-57653002018-01-17 Downregulation of CD24 suppresses bone metastasis of lung cancer Okabe, Hinako Aoki, Katsuhiko Yogosawa, Satomi Saito, Mitsuru Marumo, Keishi Yoshida, Kiyotsugu Cancer Sci Original Articles Suppression of bone metastasis can improve patient quality of life. Current drugs for bone metastasis have been shown to prolong progression‐free survival but not overall survival; therefore, other potential therapeutic targets for bone metastasis should be investigated. Cell‐surface antigens, such as CD24, have been recently shown to be involved in the metastasis of various cancers. However, whether CD24 plays a role in bone metastasis of lung cancer remains unknown. To observe metastasis of lung cancer cells by imaging technology, we introduced a near‐infrared fluorescent protein, iRFP720, into a bone‐seeking subclone established from lung cancer cells, HARA‐B4 cells. The anchorage‐independent growth of these cells was then evaluated by colony formation assays. We also compared cancer cell tropism to bone tissue with HARA‐B4 cells in the presence or absence of CD24 by cell adhesion assays. To clarify the role of CD24 in bone metastasis, we intracardially injected CD24‐knockdown HARA‐B4 cells into mice and monitored metastasis through detection of iRFP720 using an in vivo imaging system. CD24‐knockdown HARA‐B4 cells in vitro showed reduced anchorage‐independent growth and cancer cell tropism to bone. Bone metastasis was diminished in mice inoculated with CD24‐knockdown HARA‐B4 cells, which was rescued by add‐back of CD24 in cells. Our findings indicate that iRFP720 is effective for in vivo imaging analysis of bone metastasis and that downregulation of CD24 suppresses bone metastasis of lung cancer cells. These findings collectively indicate that CD24 may be considered a promising new therapeutic candidate for the prevention of bone metastasis of lung cancer. John Wiley and Sons Inc. 2017-11-29 2018-01 /pmc/articles/PMC5765300/ /pubmed/29095550 http://dx.doi.org/10.1111/cas.13435 Text en © 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Okabe, Hinako
Aoki, Katsuhiko
Yogosawa, Satomi
Saito, Mitsuru
Marumo, Keishi
Yoshida, Kiyotsugu
Downregulation of CD24 suppresses bone metastasis of lung cancer
title Downregulation of CD24 suppresses bone metastasis of lung cancer
title_full Downregulation of CD24 suppresses bone metastasis of lung cancer
title_fullStr Downregulation of CD24 suppresses bone metastasis of lung cancer
title_full_unstemmed Downregulation of CD24 suppresses bone metastasis of lung cancer
title_short Downregulation of CD24 suppresses bone metastasis of lung cancer
title_sort downregulation of cd24 suppresses bone metastasis of lung cancer
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765300/
https://www.ncbi.nlm.nih.gov/pubmed/29095550
http://dx.doi.org/10.1111/cas.13435
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