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Restorer-of-Fertility Mutations Recovered in Transposon-Active Lines of S Male-Sterile Maize

Mitochondria execute key pathways of central metabolism and serve as cellular sensing and signaling entities, functions that depend upon interactions between mitochondrial and nuclear genetic systems. This is exemplified in cytoplasmic male sterility type S (CMS-S) of Zea mays, where novel mitochond...

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Autores principales: Gabay-Laughnan, Susan, Settles, A. Mark, Hannah, L. Curtis, Porch, Timothy G., Becraft, Philip W., McCarty, Donald R., Koch, Karen E., Zhao, Liming, Kamps, Terry L., Chamusco, Karen C., Chase, Christine D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765357/
https://www.ncbi.nlm.nih.gov/pubmed/29167273
http://dx.doi.org/10.1534/g3.117.300304
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author Gabay-Laughnan, Susan
Settles, A. Mark
Hannah, L. Curtis
Porch, Timothy G.
Becraft, Philip W.
McCarty, Donald R.
Koch, Karen E.
Zhao, Liming
Kamps, Terry L.
Chamusco, Karen C.
Chase, Christine D.
author_facet Gabay-Laughnan, Susan
Settles, A. Mark
Hannah, L. Curtis
Porch, Timothy G.
Becraft, Philip W.
McCarty, Donald R.
Koch, Karen E.
Zhao, Liming
Kamps, Terry L.
Chamusco, Karen C.
Chase, Christine D.
author_sort Gabay-Laughnan, Susan
collection PubMed
description Mitochondria execute key pathways of central metabolism and serve as cellular sensing and signaling entities, functions that depend upon interactions between mitochondrial and nuclear genetic systems. This is exemplified in cytoplasmic male sterility type S (CMS-S) of Zea mays, where novel mitochondrial open reading frames are associated with a pollen collapse phenotype, but nuclear restorer-of-fertility (restorer) mutations rescue pollen function. To better understand these genetic interactions, we screened Activator-Dissociation (Ac-Ds), Enhancer/Suppressor-mutator (En/Spm), and Mutator (Mu) transposon-active CMS-S stocks to recover new restorer mutants. The frequency of restorer mutations increased in transposon-active stocks compared to transposon-inactive stocks, but most mutants recovered from Ac-Ds and En/Spm stocks were unstable, reverting upon backcrossing to CMS-S inbred lines. However, 10 independent restorer mutations recovered from CMS-S Mu transposon stocks were stable upon backcrossing. Many restorer mutations condition seed-lethal phenotypes that provide a convenient test for allelism. Eight such mutants recovered in this study included one pair of allelic mutations that were also allelic to the previously described rfl2-1 mutant. Targeted analysis of mitochondrial proteins by immunoblot identified two features that consistently distinguished restored CMS-S pollen from comparably staged, normal-cytoplasm, nonmutant pollen: increased abundance of nuclear-encoded alternative oxidase relative to mitochondria-encoded cytochrome oxidase and decreased abundance of mitochondria-encoded ATP synthase subunit 1 compared to nuclear-encoded ATP synthase subunit 2. CMS-S restorer mutants thus revealed a metabolic plasticity in maize pollen, and further study of these mutants will provide new insights into mitochondrial functions that are critical to pollen and seed development.
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spelling pubmed-57653572018-01-12 Restorer-of-Fertility Mutations Recovered in Transposon-Active Lines of S Male-Sterile Maize Gabay-Laughnan, Susan Settles, A. Mark Hannah, L. Curtis Porch, Timothy G. Becraft, Philip W. McCarty, Donald R. Koch, Karen E. Zhao, Liming Kamps, Terry L. Chamusco, Karen C. Chase, Christine D. G3 (Bethesda) Investigations Mitochondria execute key pathways of central metabolism and serve as cellular sensing and signaling entities, functions that depend upon interactions between mitochondrial and nuclear genetic systems. This is exemplified in cytoplasmic male sterility type S (CMS-S) of Zea mays, where novel mitochondrial open reading frames are associated with a pollen collapse phenotype, but nuclear restorer-of-fertility (restorer) mutations rescue pollen function. To better understand these genetic interactions, we screened Activator-Dissociation (Ac-Ds), Enhancer/Suppressor-mutator (En/Spm), and Mutator (Mu) transposon-active CMS-S stocks to recover new restorer mutants. The frequency of restorer mutations increased in transposon-active stocks compared to transposon-inactive stocks, but most mutants recovered from Ac-Ds and En/Spm stocks were unstable, reverting upon backcrossing to CMS-S inbred lines. However, 10 independent restorer mutations recovered from CMS-S Mu transposon stocks were stable upon backcrossing. Many restorer mutations condition seed-lethal phenotypes that provide a convenient test for allelism. Eight such mutants recovered in this study included one pair of allelic mutations that were also allelic to the previously described rfl2-1 mutant. Targeted analysis of mitochondrial proteins by immunoblot identified two features that consistently distinguished restored CMS-S pollen from comparably staged, normal-cytoplasm, nonmutant pollen: increased abundance of nuclear-encoded alternative oxidase relative to mitochondria-encoded cytochrome oxidase and decreased abundance of mitochondria-encoded ATP synthase subunit 1 compared to nuclear-encoded ATP synthase subunit 2. CMS-S restorer mutants thus revealed a metabolic plasticity in maize pollen, and further study of these mutants will provide new insights into mitochondrial functions that are critical to pollen and seed development. Genetics Society of America 2017-11-22 /pmc/articles/PMC5765357/ /pubmed/29167273 http://dx.doi.org/10.1534/g3.117.300304 Text en Copyright © 2018 Gabay-Laughnan et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Gabay-Laughnan, Susan
Settles, A. Mark
Hannah, L. Curtis
Porch, Timothy G.
Becraft, Philip W.
McCarty, Donald R.
Koch, Karen E.
Zhao, Liming
Kamps, Terry L.
Chamusco, Karen C.
Chase, Christine D.
Restorer-of-Fertility Mutations Recovered in Transposon-Active Lines of S Male-Sterile Maize
title Restorer-of-Fertility Mutations Recovered in Transposon-Active Lines of S Male-Sterile Maize
title_full Restorer-of-Fertility Mutations Recovered in Transposon-Active Lines of S Male-Sterile Maize
title_fullStr Restorer-of-Fertility Mutations Recovered in Transposon-Active Lines of S Male-Sterile Maize
title_full_unstemmed Restorer-of-Fertility Mutations Recovered in Transposon-Active Lines of S Male-Sterile Maize
title_short Restorer-of-Fertility Mutations Recovered in Transposon-Active Lines of S Male-Sterile Maize
title_sort restorer-of-fertility mutations recovered in transposon-active lines of s male-sterile maize
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765357/
https://www.ncbi.nlm.nih.gov/pubmed/29167273
http://dx.doi.org/10.1534/g3.117.300304
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