Cargando…

Large-Scale Low-Cost NGS Library Preparation Using a Robust Tn5 Purification and Tagmentation Protocol

Efficient preparation of high-quality sequencing libraries that well represent the biological sample is a key step for using next-generation sequencing in research. Tn5 enables fast, robust, and highly efficient processing of limited input material while scaling to the parallel processing of hundred...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hennig, Bianca P., Velten, Lars, Racke, Ines, Tu, Chelsea Szu, Thoms, Matthias, Rybin, Vladimir, Besir, Hüseyin, Remans, Kim, Steinmetz, Lars M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Genetics Society of America 2017
Materias:	Investigations
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765368/ https://www.ncbi.nlm.nih.gov/pubmed/29118030 http://dx.doi.org/10.1534/g3.117.300257

Ejemplares similares

Tn5 transposase and tagmentation procedures for massively scaled sequencing projects
por: Picelli, Simone, et al.
Publicado: (2014)

Tn5 tagments and transposes oligos to single-stranded DNA for strand-specific RNA sequencing
por: Zhang, Yanjun, et al.
Publicado: (2023)

Fast and inexpensive whole-genome sequencing library preparation from intact yeast cells
por: Vonesch, Sibylle C, et al.
Publicado: (2020)

Mosaic Ends Tagmentation (METa) Assembly for Highly Efficient Construction of Functional Metagenomic Libraries
por: Crofts, Terence S., et al.
Publicado: (2021)

Selection of Flax Genotypes for Pan-Genomic Studies by Sequencing Tagmentation-Based Transcriptome Libraries
por: Pushkova, Elena N., et al.
Publicado: (2023)

Tagmentation-based single-cell genomics
por: Adey, Andrew C.
Publicado: (2021)

Transposase-assisted tagmentation of RNA/DNA hybrid duplexes
por: Lu, Bo, et al.
Publicado: (2020)

RNA sequencing by direct tagmentation of RNA/DNA hybrids
por: Di, Lin, et al.
Publicado: (2020)

Simplified Epigenome Profiling Using Antibody-tethered Tagmentation
por: Henikoff, Steven, et al.
Publicado: (2021)

Library Preparation Based on Transposase Assisted RNA/DNA Hybrid Co-Tagmentation for Next-Generation Sequencing of Human Noroviruses
por: Zhang, Zilei, et al.
Publicado: (2021)

Efficient chromatin accessibility mapping in situ by nucleosome-tethered tagmentation
por: Henikoff, Steven, et al.
Publicado: (2020)

Profiling RNA at chromatin targets in situ by antibody-targeted tagmentation
por: Khyzha, Nadiya, et al.
Publicado: (2022)

Ultra-low-input, tagmentation-based whole-genome bisulfite sequencing
por: Adey, Andrew, et al.
Publicado: (2012)

Reconstructing and counting genomic fragments through tagmentation-based haploid phasing
por: Leong, Patrick P. T., et al.
Publicado: (2021)

A new method to prevent carry-over contaminations in two-step PCR NGS library preparations
por: Seitz, Volkhard, et al.
Publicado: (2015)

Epigenetic Application of ATAC-Seq Based on Tn5 Transposase Purification Technology
por: Li, Wangchun, et al.
Publicado: (2022)

TnT: a set of libraries for visualizing trees and track-based annotations for the web
por: Pignatelli, Miguel
Publicado: (2016)

Universal Target Capture of HIV Sequences From NGS Libraries
por: Yamaguchi, Julie, et al.
Publicado: (2018)

Tagmentation-based analysis reveals the clonal behavior of CAR-T cells in association with lentivector integration sites
por: Kim, Jaeryuk, et al.
Publicado: (2023)

Construction and characterization of a saturated Tn-seq library of Salmonella Typhimurium ATCC 14028
por: Trotereau, Jérôme, et al.
Publicado: (2023)

DNA enrichment and tagmentation method for species-level identification and strain-level differentiation using ON-rep-seq
por: Krych, Łukasz, et al.
Publicado: (2019)

TM3’seq: A Tagmentation-Mediated 3’ Sequencing Approach for Improving Scalability of RNAseq Experiments
por: Pallares, Luisa F., et al.
Publicado: (2019)

Mini-XT, a miniaturized tagmentation-based protocol for efficient sequencing of SARS-CoV-2
por: Fuchs, Marc, et al.
Publicado: (2022)

TaME-seq2: tagmentation-assisted multiplex PCR enrichment sequencing for viral genomic profiling
por: Hesselberg Løvestad, Alexander, et al.
Publicado: (2023)

Fluorescent amplification for next generation sequencing (FA-NGS) library preparation
por: Chiniquy, Jennifer, et al.
Publicado: (2020)

Diagnostic NGS for Severe Neuromuscular Disorders
por: Dhamija, Radhika, et al.
Publicado: (2015)

T-RHEX-RNAseq – a tagmentation-based, rRNA blocked, random hexamer primed RNAseq method for generating stranded RNAseq libraries directly from very low numbers of lysed cells
por: Gustafsson, Charlotte, et al.
Publicado: (2023)

A fast solution to NGS library preparation with low nanogram DNA input
por: Liu, Pingfang, et al.
Publicado: (2012)

Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing
por: Kong, Nguyet, et al.
Publicado: (2017)

Challenges in Antibody Development against Tn and Sialyl-Tn Antigens
por: Loureiro, Liliana R., et al.
Publicado: (2015)

Tn-Seq Explorer: A Tool for Analysis of High-Throughput Sequencing Data of Transposon Mutant Libraries
por: Solaimanpour, Sina, et al.
Publicado: (2015)

Mapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (ACT-seq)
por: Carter, Benjamin, et al.
Publicado: (2019)

Rapid, accurate mapping of transgene integration in viable rhesus macaque embryos using enhanced-specificity tagmentation-assisted PCR
por: Ryu, Junghyun, et al.
Publicado: (2022)

Insertion site preference of Mu, Tn5, and Tn7 transposons
por: Green, Brian, et al.
Publicado: (2012)

Je, a versatile suite to handle multiplexed NGS libraries with unique molecular identifiers
por: Girardot, Charles, et al.
Publicado: (2016)

Bead-linked transposomes enable a normalization-free workflow for NGS library preparation
por: Bruinsma, Stephen, et al.
Publicado: (2018)

A novel NGS library preparation method to characterize native termini of fragmented DNA
por: Harkins, Kelly M, et al.
Publicado: (2020)

Detection of Tn7-Like Transposons and Antibiotic Resistance in Enterobacterales From Animals Used for Food Production With Identification of Three Novel Transposons Tn6813, Tn6814, and Tn6765
por: He, Juan, et al.
Publicado: (2020)

Is amplification bias consequential in transposon sequencing (TnSeq) assays? A case study with a Staphylococcus aureus TnSeq library subjected to PCR-based and amplification-free enrichment methods
por: Alkam, Duah, et al.
Publicado: (2021)

Integration Mapping of piggyBac-Mediated CD19 Chimeric Antigen Receptor T Cells Analyzed by Novel Tagmentation-Assisted PCR
por: Hamada, Motoharu, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_bbc3cmum9q19pt04bm9usc8fvq